Phytochemical and Antioxidant-Related Investigations on Bark of Abies spectabilis (D. Don) Spach. from Nepal
Abstract
:1. Introduction
2. Results and Discussion
2.1. Antioxidant Activity and Iron (II) Chelating Capacity
Extract | DPPH IC50 μg/mL | GAE μg/g |
---|---|---|
Methanol | 4.13 ± 0.02 | 3.9 ± 0.1 |
Chloroform | >100 | n.d. |
Rutin | 4.80 ± 0.02 | n.d. |
Ascorbic acid | 3.50 ± 0.01 | n.d. |
2.2. Phytochemical Composition of Methanol Extract
[M−H]− m/z | MS2 | MS3 (parent ion) | Proposed structure |
---|---|---|---|
593 | 467; 441; 423; 305 | (467) 305; (441) 423 | G-C |
609 | 591; 483; 441; 423; 305 | (483) 305; (441) 423 | G-G |
897 | 771; 729 ; 711 | (771) 593; (729) 711 | G-C-G |
913 | 787; 745; 609 | (787) 609, 483; (745) 727 | G-G-G |
1217 | 1091; 1049; 305 | (1091) 913; (1049) 1031 | G-G-G-G |
2.3. Phytochemical Investigation of Chloroform Extract
3. Experimental
3.1. Chemicals
3.2. General Experimental Procedures
3.3. Plant Material
3.4. Extraction and Isolation
3.5. Antioxidant Activity
3.5.1. DPPH Assay
3.5.2. BR (Briggs-Rauscher Oscillating Reaction) Method
3.5.3. TEAC (Trolox Equivalent Antioxidant Capacity) Assay
3.6. Determination of Total Phenols (Total Reducing Power Quantification)
3.7. Iron (II) Chelating Capacity
4. Conclusions
Supplementary Materials
Acknowledgements
- Sample Availability: Samples of the compounds and methanol extract are available from the authors.
References and Notes
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Dall’Acqua, S.; Minesso, P.; Shresta, B.B.; Comai, S.; Jha, P.K.; Gewali, M.B.; Greco, E.; Cervellati, R.; Innocenti, G. Phytochemical and Antioxidant-Related Investigations on Bark of Abies spectabilis (D. Don) Spach. from Nepal. Molecules 2012, 17, 1686-1697. https://doi.org/10.3390/molecules17021686
Dall’Acqua S, Minesso P, Shresta BB, Comai S, Jha PK, Gewali MB, Greco E, Cervellati R, Innocenti G. Phytochemical and Antioxidant-Related Investigations on Bark of Abies spectabilis (D. Don) Spach. from Nepal. Molecules. 2012; 17(2):1686-1697. https://doi.org/10.3390/molecules17021686
Chicago/Turabian StyleDall’Acqua, Stefano, Paola Minesso, Bharat Babu Shresta, Stefano Comai, Pramod Kumar Jha, Mohan Bikram Gewali, Emanuela Greco, Rinaldo Cervellati, and Gabbriella Innocenti. 2012. "Phytochemical and Antioxidant-Related Investigations on Bark of Abies spectabilis (D. Don) Spach. from Nepal" Molecules 17, no. 2: 1686-1697. https://doi.org/10.3390/molecules17021686