Enhancement of Palmarumycin C12 and C13 Production in Liquid Culture of the Endophytic Fungus Berkleasmium sp. Dzf12 by Oligosaccharides from Its Host Plant Dioscorea zingiberensis
Abstract
:Abbreviations
OW | crude oligosaccharide prepared by acid hydrolysis of the water-extracted polysaccharide |
OS | crude oligosaccharide prepared by acid hydrolysis of the sodium hydroxide-extracted polysaccharide |
OA | crude oligosaccharide prepared by acid hydrolysis of the acid-extracted polysaccharide |
WEP | water-extracted polysaccharide |
SEP | sodium hydroxide-extracted polysaccharide |
AEP | acid-extracted polysaccharide |
TFA | trifluoroacetic acid |
1. Introduction
2. Results and Discussion
2.1. Effects of Oligosaccharides OW, OS and OA on Mycelia Growth and Palmarumycin Production
Oligo. Conc. (mg/L) | Mycelia biomass (g dw/L) | C12 content in mycelia (mg/g dw) | C13 content in mycelia (mg/g dw) | C13 yield in broth (mg/L) | C12 plus C13 yield (mg/L) | |
---|---|---|---|---|---|---|
CK | 0 | 6.84 ± 0.26 e | 1.31 ± 0.08 h | 3.24 ± 0.18 f | 108.28 ± 6.00 e | 139.45 ± 5.20 g |
OW | 200 | 11.48 ± 0.26 b | 5.40 ± 0.24 c | 8.21 ± 0.21 b | 229.06 ± 6.62 b | 385.28 ± 8.32 b |
400 | 13.90 ± 0.86 a | 6.20 ± 0.31 b | 9.92 ± 0.18 a | 245.40 ± 4.69 a | 469.33 ± 9.43 a | |
600 | 14.06 ± 0.25 a | 4.95 ± 0.18 d | 6.50 ± 0.32 c | 193.78 ± 10.41c | 369.53 ± 16.80 b | |
OS | 100 | 8.69 ± 0.26 d | 2.42 ± 0.14 g | 4.21 ± 0.14 e | 169.23 ± 7.01 d | 226.90 ± 8.35 f |
200 | 9.24 ± 0.29 cd | 5.09 ± 0.19 cd | 6.88 ± 0.06 c | 232.16 ± 9.47 ab | 340.96 ± 14.79 c | |
400 | 9.55 ± 0.47 c | 8.06 ± 0.30 a | 5.22 ± 0.06 d | 179.05 ± 7.21 d | 305.83 ± 8.69 d | |
OA | 200 | 8.80 ± 0.24 d | 2.63 ± 0.10 fg | 4.33 ± 0.16 e | 176.69 ± 8.76 d | 237.89 ± 9.33 f |
400 | 9.32 ± 0.37 cd | 3.44 ± 0.17 e | 6.29 ± 0.35 c | 241.72 ± 7.16 ab | 332.28 ± 5.92 c | |
600 | 9.82 ± 0.30 c | 2.93 ± 0.15 f | 5.15 ± 0.12 d | 205.61 ± 9.00 c | 283.56 ± 10.75 e |
2.2. Effects of Oligosaccharide OW Addition Time on Mycelia Growth and Palmarumycin Production
2.3. Kinetics of Mycelia Growth and Palmarumycin Accumulation after Treatment with Oligosaccharide OW
3. Experimental
3.1. Endophytic Fungus and Culture Conditions
3.2. Preparation of Oligosaccharides
3.3. Application of Oligosaccharides
3.4. Determination of Mycelia Biomass
3.5. Extraction and Quantification of Palmarumycins C12 and C13
3.6. Physicochemical and Spectroscopic Data of Palmarumycins C12 and C13
3.7. Measurement of Total Sugar Content
3.8. Statistical Analysis
4. Conclusions
Acknowledgements
References and Notes
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Li, Y.; Shan, T.; Mou, Y.; Li, P.; Zhao, J.; Zhao, W.; Peng, Y.; Zhou, L.; Ding, C. Enhancement of Palmarumycin C12 and C13 Production in Liquid Culture of the Endophytic Fungus Berkleasmium sp. Dzf12 by Oligosaccharides from Its Host Plant Dioscorea zingiberensis. Molecules 2012, 17, 3761-3773. https://doi.org/10.3390/molecules17043761
Li Y, Shan T, Mou Y, Li P, Zhao J, Zhao W, Peng Y, Zhou L, Ding C. Enhancement of Palmarumycin C12 and C13 Production in Liquid Culture of the Endophytic Fungus Berkleasmium sp. Dzf12 by Oligosaccharides from Its Host Plant Dioscorea zingiberensis. Molecules. 2012; 17(4):3761-3773. https://doi.org/10.3390/molecules17043761
Chicago/Turabian StyleLi, Yan, Tijiang Shan, Yan Mou, Peiqin Li, Jianglin Zhao, Wensheng Zhao, Youliang Peng, Ligang Zhou, and Chunbang Ding. 2012. "Enhancement of Palmarumycin C12 and C13 Production in Liquid Culture of the Endophytic Fungus Berkleasmium sp. Dzf12 by Oligosaccharides from Its Host Plant Dioscorea zingiberensis" Molecules 17, no. 4: 3761-3773. https://doi.org/10.3390/molecules17043761