Maturation of Murine Bone Marrow-Derived Dendritic Cells Induced by Radix Glycyrrhizae Polysaccharide
Abstract
:1. Introduction
2. Results and Discussion
2.1. GP Induces Phenotypic Maturation of DCs
2.2. GP Inhibits the Endocytic Activity of DCs
2.3. GP-Treated DCs Enhance Allogenic T Cell Proliferation
2.4. GP Enhances IL-12 Production in DCs
2.5. GP Induces IL-12 p70 Synthesis through TLR4 Signaling Pathways
2.6. Discussion
3. Experimental
3.1. Mice
3.2. GP and Other Chemicals
3.3. Induction of Mouse Bone Marrow-Derived DCs
3.4. Flow Cytometric Analysis
3.5. Endocytosis Assay
3.6. Cytokine Assays
3.7. Mixed Lymphocyte Reaction (MLR)
3.8. Real-Time PCR
3.9. Neutralization Experiments
3.10. Statistical Analysis
4. Conclusions
Acknowledgments
- Samples Availability: Samples of the Radix Glycyrrhizae polysaccharide are available from the authors.
References and Notes
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Li, X.; He, X.; Liu, B.; Xu, L.; Lu, C.; Zhao, H.; Niu, X.; Chen, S.; Lu, A. Maturation of Murine Bone Marrow-Derived Dendritic Cells Induced by Radix Glycyrrhizae Polysaccharide. Molecules 2012, 17, 6557-6568. https://doi.org/10.3390/molecules17066557
Li X, He X, Liu B, Xu L, Lu C, Zhao H, Niu X, Chen S, Lu A. Maturation of Murine Bone Marrow-Derived Dendritic Cells Induced by Radix Glycyrrhizae Polysaccharide. Molecules. 2012; 17(6):6557-6568. https://doi.org/10.3390/molecules17066557
Chicago/Turabian StyleLi, Xiaobing, Xiaojuan He, Biao Liu, Li Xu, Cheng Lu, Hongyan Zhao, Xuyan Niu, Shilin Chen, and Aiping Lu. 2012. "Maturation of Murine Bone Marrow-Derived Dendritic Cells Induced by Radix Glycyrrhizae Polysaccharide" Molecules 17, no. 6: 6557-6568. https://doi.org/10.3390/molecules17066557