Aqueous Extracts of the Edible Gracilaria tenuistipitata are Protective Against H2O2-Induced DNA Damage, Growth Inhibition, and Cell Cycle Arrest
Abstract
:1. Introduction
2. Results
2.1. Polyphenols, Flavonoids, and Ascorbic Acid Contents of AEGT and Its DPPH Radical Scavenging Activity
2.2. AEGT Modulates H2O2-Induced Plasmid DNA Strand Breaks
2.3. AEGT Modulates H2O2-Induced Cellular DNA Damage
2.4. AEGT Promotes Cell Survival under H2O2 Treatment
2.5. AEGT Prevents Cell Cycle Arrest by H2O2
2.6. Discussion
3. Experimental
3.1. Raw Materials
3.2. Extraction and Isolation of Seaweed G. tenuistipitata
3.3. Determination of Total Phenolics, Flavonoid, and Ascorbic Acid of AEGT
3.4. Free Radical Scavenging Activity
3.5. Cell Cultures
3.6. Plasmid DNA Cleavage Assay
3.7. Comet-NE Assay
3.8. Cell Viability Assay
3.9. Cell Cycle Histogram Obtained by Propidium Iodide Staining in Flow Cytometry
3.10. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Yang, J.-I.; Yeh, C.-C.; Lee, J.-C.; Yi, S.-C.; Huang, H.-W.; Tseng, C.-N.; Chang, H.-W. Aqueous Extracts of the Edible Gracilaria tenuistipitata are Protective Against H2O2-Induced DNA Damage, Growth Inhibition, and Cell Cycle Arrest. Molecules 2012, 17, 7241-7254. https://doi.org/10.3390/molecules17067241
Yang J-I, Yeh C-C, Lee J-C, Yi S-C, Huang H-W, Tseng C-N, Chang H-W. Aqueous Extracts of the Edible Gracilaria tenuistipitata are Protective Against H2O2-Induced DNA Damage, Growth Inhibition, and Cell Cycle Arrest. Molecules. 2012; 17(6):7241-7254. https://doi.org/10.3390/molecules17067241
Chicago/Turabian StyleYang, Jing-Iong, Chi-Chen Yeh, Jin-Ching Lee, Szu-Cheng Yi, Hurng-Wern Huang, Chao-Neng Tseng, and Hsueh-Wei Chang. 2012. "Aqueous Extracts of the Edible Gracilaria tenuistipitata are Protective Against H2O2-Induced DNA Damage, Growth Inhibition, and Cell Cycle Arrest" Molecules 17, no. 6: 7241-7254. https://doi.org/10.3390/molecules17067241