Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from Catharanthus roseus Leaves
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effect of Particle Size and Ethanol Concentration
2.2. Effect of Negative Pressure and Solid to Liquid Ratio
2.3. Effect of Extraction Time and Extraction Cycle
2.4. Comparison of Different Extraction Methods
Yield (mg/g) | ME | HRE | USE | NPCE |
---|---|---|---|---|
VDL | 0.5069 ± 0.0242 | 0.5138 ± 0.0253 | 0.5767 ± 0.0280 | 0.5783 ± 0.0278 |
CTR | 0.2271 ± 0.0112 | 0.2316 ± 0.0115 | 0.2811 ± 0.0134 | 0.2843 ± 0.0132 |
VCR | 0.0098 ± 0.0004 | 0.0116 ± 0.0005 | 0.0162 ± 0.0006 | 0.0181 ± 0.0007 |
VLB | 0.0603 ± 0.0028 | 0.0982 ± 0.0041 | 0.1140 ± 0.0050 | 0.1263 ± 0.0058 |
3. Experimental
3.1. Plant Material
3.2. Chemicals and Reagents
3.3. Extraction Procedures
3.3.1. Negative Pressure Cavitation Extraction (NPCE)
3.3.2. Conventional Extraction Procedures
3.4. RP-HPLC Analysis
Retention Time (min) | Peak maxima (nm) | Identity | Peak maxima (nm) in literature [ 24] |
---|---|---|---|
9.0 | 213, 251, 305 | VDL | 214, 254, 306 |
11.9 | 223, 283 | CTR | 226, 282 |
13.2 | 220, 256, 297 | VCR | 222, 256, 298 |
20.2 | 215, 265 | VLB | 214, 266 |
3.5. Statistical Analysis
4. Conclusions
Acknowledgments
References
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- Sample Availability: Samples of the VDL, CTR, VCR, VLB are available from the authors.
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Mu, F.; Yang, L.; Wang, W.; Luo, M.; Fu, Y.; Guo, X.; Zu, Y. Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from Catharanthus roseus Leaves. Molecules 2012, 17, 8742-8752. https://doi.org/10.3390/molecules17088742
Mu F, Yang L, Wang W, Luo M, Fu Y, Guo X, Zu Y. Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from Catharanthus roseus Leaves. Molecules. 2012; 17(8):8742-8752. https://doi.org/10.3390/molecules17088742
Chicago/Turabian StyleMu, Fansong, Liuqing Yang, Wei Wang, Meng Luo, Yujie Fu, Xiaorui Guo, and Yuangang Zu. 2012. "Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from Catharanthus roseus Leaves" Molecules 17, no. 8: 8742-8752. https://doi.org/10.3390/molecules17088742