The Active Ingredients of Jiang-Zhi-Ning: Study of the Nelumbo nucifera Alkaloids and Their Main Bioactive Metabolites
Abstract
:Abbreviations
DSC | differential scanning calorimetry |
HL | hyperlipidemia |
HPLC | high performance liquid chromatography |
JZN | Jiang-Zhi-Ning |
LC/MS | liquid chromatography–mass spectrometry |
2H1M | 2-hydroxy-1-methoxyaporphin |
UV | ultra-violet spectrophotometry |
1. Introduction
2. Results and Discussion
2.1. Identification and Attribution of the Effective Components of JZN
2.2. The Metabolites of 2H1M
3. Experimental
3.1. General
3.2. Identification and Attribution of Effective Ingredients of JZN
3.2.1. Experimental Animals and Sample Preparation
3.2.2. Chromatographic Conditions
Time (min) | Acetonitrile (%) | Water (0.01% Formic acid) (%) |
---|---|---|
0 | 2 | 98 |
20 | 2 | 98 |
50 | 16 | 84 |
65 | 16 | 84 |
90 | 40 | 60 |
100 | 60 | 40 |
110 | 100 | 0 |
3.2.3. Source of Precursor and Identification
3.2.4. Attribution of Precursors in Different Alkaloid Fractions of Nelumbo nucifera
3.2.5. Structure Identification of 2H1M
3.3. Experiments Investigating the Metabolites of 2H1M
3.3.1. Isolation of 2H1M
3.3.2. Plasma Sample Collection and Preparation
3.3.3. LC/MSn Conditions
4. Conclusions
Acknowledgements
References
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Chen, J.; Ma, X.; Gao, K.; Wang, Y.; Zhao, H.; Wu, H.; Wang, J.; Xie, H.; OuYang, Y.; Luo, L.; et al. The Active Ingredients of Jiang-Zhi-Ning: Study of the Nelumbo nucifera Alkaloids and Their Main Bioactive Metabolites. Molecules 2012, 17, 9855-9867. https://doi.org/10.3390/molecules17089855
Chen J, Ma X, Gao K, Wang Y, Zhao H, Wu H, Wang J, Xie H, OuYang Y, Luo L, et al. The Active Ingredients of Jiang-Zhi-Ning: Study of the Nelumbo nucifera Alkaloids and Their Main Bioactive Metabolites. Molecules. 2012; 17(8):9855-9867. https://doi.org/10.3390/molecules17089855
Chicago/Turabian StyleChen, Jianxin, Xueling Ma, Kuo Gao, Yong Wang, Huihui Zhao, Hao Wu, Juan Wang, Hua Xie, Yulin OuYang, Liangtao Luo, and et al. 2012. "The Active Ingredients of Jiang-Zhi-Ning: Study of the Nelumbo nucifera Alkaloids and Their Main Bioactive Metabolites" Molecules 17, no. 8: 9855-9867. https://doi.org/10.3390/molecules17089855