The Identification and Characterization of Chitotriosidase Activity in Pancreatin from Porcine Pancreas
Abstract
:1. Introduction
2. Results and Discussion
2.1. Sensitivity of In-gel Chitinase and Chitosanase Assays
2.2. Unusual Susceptibilities of Chitin and Chitosan to Enzymatic Hydrolysis
2.3. Characteristics of Pancreatin
Substrate | Specific activity (U/mg) |
---|---|
PNP-GlcNAc | 0 |
PNP-(GlcNAc)2 | 0.1949 ± 0.0072 |
PNP-(GlcNAc)3 | 0.1534 ± 0.0099 |
Substrate | Specific activity (U/mg) |
---|---|
α-chitin | 0.0154 ± 0.0019 |
β-chitin | 0.0337 ± 0.0019 |
3. Experimental
3.1. Materials
3.2. Preparation of Glycol Chitin and Glycol Chitosan
3.3. In-gel Assays of Chitin or Chitosan
3.4. Chitinase Activity Assay
3.5. Substrate Specificity Assay
4. Conclusions
Acknowledgments
References
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Shen, C.-R.; Liu, C.-L.; Lee, H.-P.; Chen, J.-K. The Identification and Characterization of Chitotriosidase Activity in Pancreatin from Porcine Pancreas. Molecules 2013, 18, 2978-2987. https://doi.org/10.3390/molecules18032978
Shen C-R, Liu C-L, Lee H-P, Chen J-K. The Identification and Characterization of Chitotriosidase Activity in Pancreatin from Porcine Pancreas. Molecules. 2013; 18(3):2978-2987. https://doi.org/10.3390/molecules18032978
Chicago/Turabian StyleShen, Chia-Rui, Chao-Lin Liu, Hsiao-Ping Lee, and Jeen-Kuan Chen. 2013. "The Identification and Characterization of Chitotriosidase Activity in Pancreatin from Porcine Pancreas" Molecules 18, no. 3: 2978-2987. https://doi.org/10.3390/molecules18032978