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Review

Click-to-Chelate: Development of Technetium and Rhenium-Tricarbonyl Labeled Radiopharmaceuticals

by
Christiane A. Kluba
and
Thomas L. Mindt
*
Division of Radiopharmaceutical Chemistry, Department of Radiology and Nuclear Medicine, University of Basel Hospital, Petersgraben 4, 4031 Basel
*
Author to whom correspondence should be addressed.
Molecules 2013, 18(3), 3206-3226; https://doi.org/10.3390/molecules18033206
Submission received: 20 February 2013 / Revised: 5 March 2013 / Accepted: 6 March 2013 / Published: 12 March 2013
(This article belongs to the Collection Advances in Click Chemistry)
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Abstract

:
The Click-to-Chelate approach is a highly efficient strategy for the radiolabeling of molecules of medicinal interest with technetium and rhenium-tricarbonyl cores. Reaction of azide-functionalized molecules with alkyne prochelators by the Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC; click reaction) enables the simultaneous synthesis and conjugation of tridentate chelating systems for the stable complexation of the radiometals. In many cases, the functionalization of (bio)molecules with the ligand system and radiolabeling can be achieved by convenient one-pot procedures. Since its first report in 2006, Click-to-Chelate has been applied to the development of numerous novel radiotracers with promising potential for translation into the clinic. This review summarizes the use of the Click-to-Chelate approach in radiopharmaceutical sciences and provides a perspective for future applications.

Graphical Abstract

1. Introduction

Even though new generator-based radionuclides (e.g., 68Ga) are emerging [1], technetium 99m remains the workhorse of nuclear medicine due to its convenient availability via 99mMo/99mTc generators, reasonable cost, and ideal decay characteristics (t1/2 = 6.02 h, 140 keV, 89% abundance) for Single Photon Emission Computed Tomography (SPECT). Approximately 80% of all routine nuclear imaging applications are based on 99mTc, which translates into estimated 50 million doses of diagnostic radiopharmaceuticals annually worldwide, or more than 50,000 applications per day in the US alone [2,3]. In addition, the availability of β-particle-emitting rhenium 186/188 as group 7 transition metal congeners of 99mTc with similar chemical properties provides a “matched pair” constellation of radionuclides for both diagnostic and therapeutic applications. The possibility to radiolabel molecules of medicinal interest with diagnostic or therapeutic radionuclides by the same chemistry approach has recently received considerable interest in the field of nuclear medicine for the development of theranostic agents [4].
Because of the diverse redox chemistry of technetium and rhenium, a wealth of complexes differing in coordination numbers and geometries has been reported. Here, we focus on the technetium and rhenium-tricarbonyl cores ([M(H2O)3(CO)3]+, or short [M(CO)3]+; M = 99mTc(I), 186/188/natRe(I)). 99mTc- and 186/188Re-tricarbonyl can be prepared by reduction of the corresponding permetallates ([M(VII)O4]−) using K2[H3BCO2] (e.g., by the commercial IsoLinkTM kit; Scheme 1). [M(CO)3]+ contains three tightly coordinated CO ligands and three water molecules, the latter of which can be readily replaced by mono-, bi-, and tridentate ligand systems, or a combination thereof. Complexes of [M(CO)3]+ exhibit a low spin d6 configuration, which renders the metal inert and hence, provides complexes with high in vivo stability essential for medical applications [5]. In addition, octahedral complexes of [M(CO)3]+ are generally smaller than octahedral or square-pyramidal complexes of the corresponding metals in higher oxidation states (e.g., M(V)-oxo cores) and are therefore considered less likely to impact important characteristics of (bio)molecules to which they are conjugated to.
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Scheme 1. Synthesis of [M(CO)3]+ from permetallates and subsequent complexation with ligands (L). (a) For M = 99mTc: (i) IsoLinkTM kit (containing K2[H3BCO2]), saline, 100 °C, 20 min; for M = 188Re: (i) SnCl2, gluconate, H3PO4; (ii) K2[H3BCO2].
Scheme 1. Synthesis of [M(CO)3]+ from permetallates and subsequent complexation with ligands (L). (a) For M = 99mTc: (i) IsoLinkTM kit (containing K2[H3BCO2]), saline, 100 °C, 20 min; for M = 188Re: (i) SnCl2, gluconate, H3PO4; (ii) K2[H3BCO2].
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Since the first report of the 99mTc-tricarbonyl core [99mTc(H2O)3(CO)3]+ in 1998 by Alberto and Schibli et al. [6] numerous ligand systems and their coordination with [M(CO)3]+ have been investigated. In particular, tridentate chelators containing N-, S-, or O-donors have been shown to provide well-defined and stable organometallic complexes, which are suitable for application in vivo (Figure 1) [7,8] Also, functionalized η5-ligand cyclopentadienide as well as carboranes [9] have been used for the stable complexation of the tricarbonyl core. A thorough discussion of the literature on ligand systems reported for the complexation of [M(CO)3]+ is beyond the scope of this article and instead, it is referred to some excellent reviews on the general topic of 99mTc chelation chemistry (see e.g., [10]).
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Figure 1. Representative examples of bifunctional chelating agents (BFCAs) for the complexation of the [99mTc(CO)3]+ core and conjugation to (bio)molecules.
Figure 1. Representative examples of bifunctional chelating agents (BFCAs) for the complexation of the [99mTc(CO)3]+ core and conjugation to (bio)molecules.
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Radiolabeling of molecules of medicinal interest with [M(CO)3]+ is usually achieved by a post-labeling approach using bifunctional chelating agents (BFCAs). As the name implies, BFCAs enable both the covalent linkage to a (bio)molecule and the coordination of the radiometal. For many of the ligand systems reported for [M(CO)3]+, appropriately functionalized derivatives for conjugation to (bio)molecules via different functional groups (e.g., amines, carboxylates, thiols) have been reported [10]. However, the multifunctional character of both the ligand system and (in most cases) the (bio)molecule of interest can make a selective conjugation in solution a difficult endeavor. To overcome these problems, protective group strategies are often employed; though, such approaches usually result in multi-step reaction sequences, which can significantly lower the overall efficiency of the labeling procedure.
Despite the success of the metal tricarbonyl cores in the field of radiopharmaceutical sciences, there is still a need for novel and innovative strategies for (bio)conjugation techniques and radiolabeling procedures in order to expedite the development of radiotracers based on [M(CO)3]+. In the following, this review will focus on such a recently developed approach that utilizes click chemistry in this context.

2. Click Chemistry in Radiopharmaceutical Sciences

Click chemistry, a term minted by Sharpless [11] et al. in 2001, is an attempt to define the characteristics of a perfect (or nearly perfect) chemical reaction that generates structurally diverse new substances by joining small building blocks in a quick, selective, efficient, and reliable manner. The prime example of a click reaction within the click chemistry concept is the copper (Cu(I))-catalyzed azide-alkyne cycloaddition (CuAAC), reported at the same time by the groups of Sharpless and Meldal in 2002 [12,13]. The CuAAC proceeds efficiently and selectively under mild reaction conditions (e.g., in water at room temperature) in the presence of other functional groups, and provides a stable 1,4-disubstituted 1,2,3-triazole heterocyclic linkage between the reaction partners. Not surprisingly, the attractive features of this click reaction have inspired numerous applications across different scientific disciplines [14,15,16], including examples of the development of radiotracers.
The first examples on the use of the CuAAC in radiopharmaceutical sciences were published in 2006. Kolb et al. [17] and Marik et al. [18] described the click reaction of [18F]fluorinated alkyne derivatives with azide acceptors, such as azido thymidine (AZT) and azide-functionalized peptides. These applications employ a two-step prosthetic group approach, which enables the efficient and selective 18F radiolabeling of molecules in aqueous media. For an overview on the CuAAC-mediated radiolabeling of molecules with 18F- (and 11C-) radionuclides, it is herein referred to some of the several recent review articles on the topic [19,20].
Also in 2006, Mindt and Schibli et al. reported the first example of an application of the CuAAC to metallic radionuclides [21]. In their approach, the click reaction is employed to the synthesis of tridentate ligand systems for the stable complexation of [99mTc(CO)3]+ while conjugating them simultaneously to (bio)molecules. Unlike subsequently published applications of the CuAAC for the labeling of molecules with radiometals, their approach utilizes the 1,2,3-triazole not only as a mere linker to connect a molecule with a radiometal chelate (conjugate design), but makes use of the excellent coordination properties of the heterocycle as part of a functional ligand system in an integrated approach [22]. The broad applicability and efficiency of this 99mTc-radiolabeling strategy, in particular those provided by convenient one-pot procedures developed in the course of the work, prompted the authors to term their approach “Click-to-Chelate” [21]. This review summarizes original articles on the Click-to-Chelate concept published since 2006. Examples of applications of the CuAAC in which the 1,2,3-triazole is not part of a chelating system but just tethers a radiometal complex to various molecules are not included. Particular attention has been paid to examples of “clicked” 99mTc-radiotracers which describe biological evaluations in vitro and in vivo, as this is an important aspect for potential translation of the compounds into the clinic.

3. Click-to-Chelate

As discussed in the introduction, the preparation of tridentate chelating systems (and BFCAs thereof) for the complexation of [M(CO)3]+ generally requires multi-step synthesis and their incorporation into (bio)molecules often lacks efficiency and selectivity due to cross-reactivity with other functional groups present. The chemical orthogonality of the CuAAC together with the ease of the introduction of alkyne and azide moieties into molecules by synthetic or biochemical methods [23,24,25,26,27] provides a solution to these shortcomings. Thus, the reaction of azide-functionalized compounds with alkyne prochelators provides 1,4-disubstituted 1,2,3-triazole-containing tridentate ligand systems (termed “regular click ligands”; Scheme 2A) [21]. Similarly, cycloaddition of alkyne derivatives with azide prochelators yields isomeric triazolyl products (referred to as “inverse click ligands”; Scheme 2B), which are set up to coordinate to [M(CO)3]+ via the nitrogen in position 2 of the triazole heterocycle, rather than the nitrogen in position 3 of “regular click ligands” (see below). Noteworthy, all ligand systems can be prepared without the use of protective groups in a single step, high yields, and under mild reaction conditions suitable for the functionalization of delicate biomolecules. Reaction of the tridentate chelators with [M(CO)3]+ gives structurally diverse organometallic complexes that differ in size, lipophilicity, and overall charge (Table 1). All these characteristics are important for fine tuning the physicochemical properties of a radiotracer for its application in vivo.
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Scheme 2. Schematic representation of the synthesis of regular (A) and inverse (B) 1,2,3-triazolyl ligand systems for the complexation of the [M(CO)3]+ core. A and B represents various functional groups for coordination of the tricarbonyl core.
Scheme 2. Schematic representation of the synthesis of regular (A) and inverse (B) 1,2,3-triazolyl ligand systems for the complexation of the [M(CO)3]+ core. A and B represents various functional groups for coordination of the tricarbonyl core.
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The radiolabeling of regular click ligands (L; Table 1, entries 1–5) with [99mTc(CO)3]+ proceeds smoothly under standard reactions conditions (PBS, pH 7.4, 100 °C, 20–30 min) [21]. Quantitative formation of [99mTc(CO)3(L)] can be achieved at a ligand concentration in the micromolar range (10–5–10–6 M), which is comparable to that of histidine (or Nτ-substituted histidines), one of the most efficient chelators for complexation of the 99mTc tricarbonyl core [28]. Particularly for receptor targeting radiopharmaceuticals, it is important that high specific activities can be achieved (e.g., by employing low ligand concentrations) in order to avoid interfering receptor saturation. On the other hand, quantitative radiolabeling of the 99mTc-tricarbonyl core with inverse click ligands (e.g., Table 1, entry 6; for additional examples see ref. [21]) requires a ligand concentration orders of magnitudes higher (10–3–10–2 M) than that of regular click ligands. This indicates a decreased efficiency of the inverse click ligand systems likely due to the lower electron density at N2 compared to that of N3 of the triazole heterocycle as shown by density functional theory (DFT) calculations [21].
Brans et al. have conjugated both variations of click ligands to the N-terminus of a tumor-targeting bombesin (BBS) derivative (Scheme 3; see below for the relevance of BBS in nuclear oncology) [29]. Radiolabeling of the two peptides with [99mTc(CO)3]+ under standard conditions revealed a significantly lower radiolabeling yield for the peptide with the inverse click ligand (<60%) in comparison to the compound with the regular click ligand (>95%). Moreover, the 99mTc adduct of the inverse ligand system was found to be unstable and thus, not suitable for in vivo evaluation. Anderson et al. have reported a similar instability of a natRe-tricarbonyl based luminescent probe in which the metal is coordinated by the N2 nitrogen of triazole ligands [30]. Even though different metal complexes of 1,4-disubstituted 1,2,3-triazoles with coordination can be synthesized via the N2 [31], the low chelation efficiency combined with the modest stability of the complexes in biological media makes them unlikely to find biological or medical applications. It is therefore important that 1,2,3-triazole-based metallic bioprobes are designed in a way that ensures coordination via the N3 of the heterocycle.
Table
Table 1. Examples of prochelators, formation of tridentate triazolyl ligands by CuAAC with benzyl azide (entry 1–5) or 3-phenyl-1-propyne (entry 6), and complexes with [M(CO)3]+ thereof.
Table 1. Examples of prochelators, formation of tridentate triazolyl ligands by CuAAC with benzyl azide (entry 1–5) or 3-phenyl-1-propyne (entry 6), and complexes with [M(CO)3]+ thereof.
EntryProchelatorTridentate ligand (L) 1Metal complex 2
13 Molecules 18 03206 i004 Molecules 18 03206 i005 Molecules 18 03206 i006
24 Molecules 18 03206 i007 Molecules 18 03206 i008 Molecules 18 03206 i009
3 Molecules 18 03206 i010 Molecules 18 03206 i011 Molecules 18 03206 i012
4 Molecules 18 03206 i013 Molecules 18 03206 i014 Molecules 18 03206 i015
5 Molecules 18 03206 i016 Molecules 18 03206 i017 Molecules 18 03206 i018
63, 5 Molecules 18 03206 i019 Molecules 18 03206 i020 Molecules 18 03206 i021
(1) Coordinating nitrogen of the 1,2,3-triazole heterocycle is indicated; (2) M = 99mTc, natRe; (3) commercial compounds; (4) prochelators derived from amino acids (R = amino acid specific side chain); (5) example of an inverse click ligand.
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Scheme 3. Regular and inverse click ligands conjugated to the N-terminus of a bombesin derivative. Peptide = (ßAla)1–2[Cha13, Nle14]BBS(7–14); the N-terminal ßAla units serve as a spacer between the peptide and the metal chelate.
Scheme 3. Regular and inverse click ligands conjugated to the N-terminus of a bombesin derivative. Peptide = (ßAla)1–2[Cha13, Nle14]BBS(7–14); the N-terminal ßAla units serve as a spacer between the peptide and the metal chelate.
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It is common practice to identify 99mTc-complexes prepared on a no carrier added (n.c.a.) level by comparison with the corresponding non-radioactive natRe analogues by HPLC (γ-trace versus UV-trace; Scheme 4). In addition, the corresponding natRe-tricarbonyl complexes allow structural analysis by spectrometric and spectroscopic methods including IR, MS, and NMR. natRe analogues of 99mTc-tricarbonyl complexes can be synthesized conveniently on a macroscopic scale by the reaction of the ligand system of interests with [Et4N]2[Re(CO)3(Br)3] [32] in alcohol or water according to published procedures. In all cases discussed above (and more examples have been reported in the meantime), the corresponding natRe-tricarbonyl complexes were prepared and fully characterized. In addition, more than half a dozen reported X-ray structures confirm the formation of fac-M(CO)3 complexes by tridentate coordination of the triazolyl ligands as depicted in, e.g., Figure 1, Table 1, or Scheme 5B [33,34,35,36].
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Scheme 4. Labeling of a representative example of a regular click ligand with 99mTc- and natRe-tricarbonyl for identification and characterization of the organometallic products.
Scheme 4. Labeling of a representative example of a regular click ligand with 99mTc- and natRe-tricarbonyl for identification and characterization of the organometallic products.
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Molecules 18 03206 g013 550
Scheme 5. Extension of the Click-to-Chelate approach provides efficiently trifunctional (A), or tetrafunctional (B) conjugates. TMS = trimethylsilyl group.
Scheme 5. Extension of the Click-to-Chelate approach provides efficiently trifunctional (A), or tetrafunctional (B) conjugates. TMS = trimethylsilyl group.
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As pointed out above, a large number of non-radioactive natRe-tricarbonyl complexes have been reported for structural elucidation and identification of the analogous 99mTc complexes. Only recently have Wang et al. reported the first example of a chelate in which the 188Re-tricarbonyl core is coordinated by a click ligand (Figure 2) [37]. In their work, the efficiency of the complexation of [188Re(CO)3(H2O)3]Br with bis(pyridine-2-ylmethyl)amine (dpa) was compared with that of a regular click ligand. Even though dpa was shown to be the more efficient chelator, respectable radiolabeling yields (>80%) were achieved with the regular click ligand system.
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Figure 2. Fac-188Re-tricarbonyl complexes of dpa and a regular click ligand.
Figure 2. Fac-188Re-tricarbonyl complexes of dpa and a regular click ligand.
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4. One-Pot Click-to-Chelate Procedures

While the ligands described above, in particularly the regular click ligands, represent a class of excellent chelators for the complexation of [M(CO)3]+, the individual azide and alkyne substrates as well as the various additives required for CuAAC are poor ligands and do not form stable or defined complexes with the organometallic tricarbonyl core [21]. Exploitation of this observation led to the development of efficient procedures by which the synthesis of the ligand system, its conjugation to a molecule of interest, and the radiolabeling with [M(CO)3]+ can be achieved in a single pot without isolation and purification of intermediates. Thus, heating of an aqueous solution of azide substrates and alkyne prochelators in the presence of catalytic amounts of Cu(I) for 20–30 min at 100 °C followed by addition of [99mTc(CO)3]+ and a second heating step (Scheme 6A) provided cleanly the same 99mTc tricarbonyl labeled compounds in identical radiochemical yields and purity as obtained by the reaction of pre-synthesized and isolated triazolyl ligands with [99mTc(CO)3]+ (Scheme 6C). Remarkably, the same 99mTc-tricarbonyl complexes can be obtained in some instances by simply mixing all substrates with the generator eluent containing pertechnetate ([99mTcO4]–) in the presence of the IsoLink™ kit (Scheme 6B). In the meantime, a number of examples of successful one-pot, two-step- or single-step procedures have been reported for different click-ligand systems (see below). In some cases, the reaction time of individual steps can be accelerated by microwave heating (unpublished results). Simplification of radiolabeling procedures as illustrated by the one-pot examples shown are of fundamental importance for facilitating the translation of novel labeling strategies into routine practice for clinical applications in nuclear medicine.
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Scheme 6. Example of one-pot, two-step (A) and one-pot, single-step (B) Click-to-Chelate procedures yielding the same radiolabeled compounds as obtained by multi-step synthesis with pre-synthesized and isolated triazolyl ligand systems (C).
Scheme 6. Example of one-pot, two-step (A) and one-pot, single-step (B) Click-to-Chelate procedures yielding the same radiolabeled compounds as obtained by multi-step synthesis with pre-synthesized and isolated triazolyl ligand systems (C).
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5. Comparison of Click Ligands with Established Chelators for [M(CO)3]+

As true for any new ligand system designed for the formation of radiometal complexes for use in nuclear medicine, its performance should be scrutinized and compared with established systems in order to evaluate its general utility. For regular click ligands, this was accomplished by a direct side-by-side comparison in vitro and in vivo with two gold standard chelators for [99mTc(CO)3]+, namely Nτ-derivatised histidine (His) and Nα-acetylated His (Figure 3). Towards this goal, Mindt et al. [38] Brans et al. [29], and Zhang et al. [39] have applied the click-to-chelate approach to tumor-targeting vectors of relevance in nuclear oncology and studied the biological characteristics of the 99mTc-labeled conjugates.
Radiolabeled folic acid derivatives functionalized at the γ-position with a radiometal chelate have been shown to be promising candidates for the development of radiotracers specific for folate receptors (FR), which are overexpressed by tumors of the ovary, cervix, endometrium, lung, kidney, breast, colon, and brain [40]. Bombesin (BBS) is a peptide, which targets the gastrin-releasing peptide receptor (GRPr). BBS and derivatives thereof are interesting vectors for the development of tumor targeting agents because of the overexpression of the GRPr by different tumor cells including prostate, breast, colon, and small-cell lung carcinomas [41]. Finally, the peptide KCCYSL, identified by phage display, is a small peptide specific for the ErbB-2 receptor, a member of the epidermal growth factor receptor family, which is overexpressed by breast and prostate carcinoma cells [39].
The comparative studies of the 99mTc-tricarbonyl labeled folate (Figure 3A), BBS, and ErbB-2-targeting peptide derivatives (Figure 3B) clearly demonstrated the equivalence of the clicked ligand system versus the established Nτ-derivatized His and Nα-acetylated His chelators. Radiolabeling yields and purities achieved with the conjugates bearing a regular click ligand were equal to that of the conjugates equipped with the reference chelators, as were their blood serum stabilities, cell internalization properties, receptor affinities, and receptor specificities. Most importantly, the nature of the ligand system did not influence the biodistribution of the radioconjugates as determined by experiments in vivo with the corresponding mouse models.
Also, Ferro-Flores et al. have compared a bombesin derivative, [Lys3]BBS(1–14), radiolabeled with [99mTc(CO)3]+ via a regular click ligand with an analogous compound radiolabeled with technetium(III) using the EDDA/HYNIC (ethylenediamine N,N'-diacetic acid/hydrazinonicotinamide) chelating system [42]. As expected, the 99mTc-tricarbonyl radiopeptide exhibited an increased lipophilicity and thus, higher hepatobiliary excretion than the EDDA/HYNIC derivative. The authors conclude that the BBS conjugate radiolabeled with the 99mTc-tricarbonyl core via a regular click ligand is nevertheless the more promising candidate for clinical development because, unlike in the case of HYNIC chelates [43], it consists of a structurally defined metal complex on a tracer level, an important prerequisite for the approval of new radiopharmaceuticals by regulatory authorities.
It is important to note that Click-to-Chelate not only offers an efficient and convenient way for the introduction of a ligand system into (bio)molecules of interest and their radiolabeling with [99mTc(CO)3]+ but also reduces significantly the synthetic effort to obtain the corresponding precursors, e.g., alkyne prochelators (or BFCAs with respect to other ligand systems). This benefit of Click-to-Chelate may not be obvious in the case of the modification of peptides to which different entities, including various ligand systems, can conveniently conjugated to on solid support. However, the advantages of Click-to-Chelate become more apparent when applied to the synthetically more challenging situation of the selective modification of polyfunctional molecules in solution. For example, the synthesis of 1,2,3-triazole-modified folic acid (Figure 3A) was achieved by Click-to-Chelate in eight synthetic steps with an overall yield of approx. 80% [38]. In comparison, the analogous 99mTc-tricarbonyl labeled folic acid derivative equipped with an Nτ-derivatized His chelator required ten synthetic steps and provided the final radiotracer in < 1% yield [44]. This example, as well as others discussed below, demonstrates the synthetic efficiency of the Click-to-Chelate strategy for the assembly of 99mTc tricarbonyl radiotracers.
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Figure 3. Comparison of regular click ligands with established chelators for the labeling of tumor-seeking vectors with the 99mTctricarbonyl core. (A) Folic acid conjugated to a Nτ-derivatized His chelator or a regular click ligand. (B) Two peptides functionalized with a NαAc His chelator or a regular click ligand. The ßAla and the GSG motifs are spacer units that separate the receptor targeting peptide from the chelate.
Figure 3. Comparison of regular click ligands with established chelators for the labeling of tumor-seeking vectors with the 99mTctricarbonyl core. (A) Folic acid conjugated to a Nτ-derivatized His chelator or a regular click ligand. (B) Two peptides functionalized with a NαAc His chelator or a regular click ligand. The ßAla and the GSG motifs are spacer units that separate the receptor targeting peptide from the chelate.
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6. Multifunctional Radioconjugates by Extended Click-to-Chelate

So far, the synthesis of tridentate 1,4-disubstituted 1,2,3 triazole-containing ligand systems and their simultaneous conjugation to molecules of interest by means of the CuAAC have been discussed. The Click-to-Chelate approach can be readily extended to the preparation of multifunctional 99mTc-tricarbonyl radioconjugates by structural modifications of the prochelators employed. Mindt et al. have demonstrated the feasibility of such applications by two different approaches [34,36]. In a first strategy, a lysine-derived prochelator is employed to which various molecules of interest (R1) can be selectively conjugated to via the Nε-amine functionality by amide bond formation (Scheme 5A). CuAAC with a second, azide-functionalized molecule (R2) covalently links the two molecules via the formed regular click ligand. Thus, the final product combines a nuclear reporter probe (99mTc) with two different chemical (or biological) entities in a trifunctional conjugate. The strategy was successfully applied to the preparation of a 99mTc-labeled conjugate comprising a tumor-targeting peptide sequence ([Cha13, Nle14]bombesin(7–14)) and a low molecular weight albumin binder, a pharmacological modifier to elongate the blood circulation time of the conjugate. Evaluation of the trifunctional conjugate in vitro and in vivo provided promising results for its use as a SPECT imaging agent for the visualization of GRPr-positive tumors [34]. Recently, Kluba et al. have reported yet another application of this strategy by combining the 99mTc-tricarbonyl core with two moieties, each specific for an extra- and intracellular target, respectively [45]. The trifunctional conjugate was designed to target cancer cells by interaction of a BBS fragment (R2) with GRPr and, after internalization, with mitochondria via an organelle-specific triphenylphosphonium moiety (R1). Adding a moiety specific for an intracellular target to a tumor-targeting peptide conjugate could improve the cellular retention of radioactivity after its successful delivery to tumors and metastases and thus, improve the efficacy of radiopeptide conjugates.
The second approach to multifunctional conjugates involves chelators containing two 1,4-disubtituted 1,2,3-triazoles derived from the CuAAC of azides with a bis-alkyne precursor (Scheme 5B) [36]. The latter can be readily obtained by the stepwise Nα-alkylation of, e.g., glycine with propargyl bromide or silyl protected derivatives thereof. One molecule (R1) can be selectively linked to the glycine precursor by amide bond formation. The other two molecules (R2 and R3) are conjugated by sequential CuAAC, first with the terminal alkyne and, after removal of the silyl protective group, with the second alkyne functionality. Thus, this strategy provides access to overall tetrafunctional conjugates by joining three different chemical or biochemical moieties with the 99mTc-tricarbonyl core. The utility of the approach was demonstrated by the conjugation of a GRPr targeting bombesin derivative with a related bis-triazole ligand system to which two carboxylates were linked to by the CuAAC [36]. The observed low renal uptake and retention of the conjugate in vivo suggests that the two appending carboxylates act as pharmacological modulators, which successfully masked the otherwise unfavorable positive charge of the conjugate as the result of the cationic metal chelate.
An almost infinite number of possible combinations of different chemical or biological moieties with the 99mTc-tricarbonyl core can be envisioned by the extended Click-to-Chelate approach and potential applications to radiopharmaceutical development are galore. For example, the combination of tumor-avid vectors with pharmacological modifiers could lead to the development of radiotracers with improved pharmacological profiles, whereas the use of additional imaging probes or therapeutic agents may lead to the design of multimodal conjugates or theranostics. The exploitation of the strategies described has just begun and it is possible that ongoing research efforts directed towards the combination of different entities with the 99mTc-tricarbonyl core will lead to the development of novel radiopharmaceuticals with improved properties for application in molecular imaging and/or receptor-mediated radionuclide therapy.

7. Applications of Click-to-Chelate to Radiotracer Development

In the following section, various reported applications of Click-to-Chelate to the development of radiotracers will be summarized, some examples of which have been mentioned already in the text above (including an example of a vitamin, namely folic acid, vitamin B9) [38]. Compounds reported without preclinical in vitro and/or in vivo data were not included (e.g., “clicked” phospholipids) [21].
Click-to-Chelate has been successfully applied to the 99mTc-tricarbonyl labeling of different tumor avid peptides including derivatives of bombesin and the ErbB-2-targeting peptides (see above). In these studies, the general utility of “clicked” triazolyl ligand systems for applications to 99mTc-tricarbonyl labeled peptides and multifunctional radioconjugates was demonstrated. A further example has been reported by Kim et al. (Figure 4) who have labeled an epitope-mimicking peptide of the hepatocyte growth factor (HGF)-mesenchymal-epithelial transition factor (Met) molecular pathway, which affects cancer development at different stages [46]. In their work, the length of a spacer unit that separates the c-met binding peptide from the 99mTc-tricarbonyl chelate was investigated. They have shown that the modification of the peptide was readily accomplished by Click-to-Chelate providing radiopeptides of good stability and excellent binding affinity to the c-met receptor. Based on the in vitro evaluation of the radiopeptides investigated, a spacer unit consisting of three glycine units (n = 3) provided the most promising results.
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Figure 4. Investigations of the effect of the length of the spacer between a clicked-99mTc-tricarbonyl chelate and the tumor targeting c-met binding peptide.
Figure 4. Investigations of the effect of the length of the spacer between a clicked-99mTc-tricarbonyl chelate and the tumor targeting c-met binding peptide.
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Glucose is considered as a key molecule in metabolic evaluation of tumors due to the up-regulation of glycolysis, which results in an increased consumption of the carbohydrate in tumor tissue [47]. In the past, different strategies have been reported for the identification of a 99mTc labeled glucose derivative as a more readily available SPECT surrogate of the established PET tracer [18F]-2-fluoro-2-deoxy-D-glucose (FDG) [48]. Fernández et al. have prepared a glucose derivative functionalized at the C2 position by the one-pot, 2-step Click-to-Chelate protocol (Figure 5) and studied its biodistribution in comparison to FDG in a lung carcinoma mouse model [49]. Reported tumor uptake in vivo of the radiotracer was moderate in comparison to FDG and likely due to passive diffusion of the hydrophilic compound. As in the case of other reported 99mTc/natRe-labeled glucose derivatives, specific uptake via the GLUT-1 receptor pathway could not be confirmed.
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Figure 5. A glucose derivative functionalized at the C2 position with a regular click ligand by the one-pot, 2-step Click-to-Chelate procedure.
Figure 5. A glucose derivative functionalized at the C2 position with a regular click ligand by the one-pot, 2-step Click-to-Chelate procedure.
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Click-to-Chelate has also been employed to the development of hypoxia imaging agents, which are important radiotracers for the assessment of the oxygenation status of tumors critical for determining the effectiveness of radiation therapy [50]. Fernández et al. have investigated the utility of bi- and tridentate click chelators for the 99mTc-tricarbonyl labeling of 5-nitro-imidazole derivatives prepared by one-pot Click-to-Chelate protocols (Figure 6) [51]. Evaluation of the compounds in vitro and in vivo revealed that the compound equipped with a tridentate chelator was superior in terms of blood serum stability, plasma protein binding, and tumor uptake. In addition, the “clicked” 5-nitro-imidazole outperformed other 99mTc-labeled hypoxia imaging agents previously investigated by the same group (e.g., 5-nitroimidazol labeled with 99mTc(V) nitrido- or 99mTc(III) [4+1] chelates). The authors conclude that the compound with a tridentate regular click ligand is so far their most promising candidate for the development of a 99mTc radiopharmaceutical for hypoxia tumor diagnosis.
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Figure 6. Comparison of tri- and bidentate click chelators applied to the development of novel hypoxia radiotracers based on 5-nitro-imidazoles.
Figure 6. Comparison of tri- and bidentate click chelators applied to the development of novel hypoxia radiotracers based on 5-nitro-imidazoles.
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Analogs of the nucleoside thymidine have the potential to be recognized as substrates by the human cytosolic thymidine kinase (hTK1). Since hTK1 is overexpressed in a wide variety of cancer cells, radiolabeled thymidine derivatives represent potential markers for cancer cell proliferation. Struthers et al. have reported the synthesis and in vitro evaluation of 99mTc-tricarbonyl labeled thymidine analogs in an effort to develop a radiotracer for SPECT more readily available compared to the currently used PET tracer 3-deoxy-3'-[18F]fluorothymidine (FLT) [35,52]. They prepared a set of 99mTc-tricarbonyl labeled thymidine derivatives in a combinatorial fashion by parallel one-pot Click-to-Chelate synthesis (Scheme 7; see Table 1, entry 1–5 for examples of alkyne precursors employed). The products obtained differ not only in the position of functionalization (C3' versus N3) but also with regards to the size and overall charge of the radiometal chelate. Substrate activity studies carried out by phosphorylation assays showed that substrate recognition by hTK1 is dependent on both, the site of functionalization of thymidine as well as the charge of the 99mTccomplex. In general, C3'-functionalized compounds were the better substrates for hTK1 and a preference for neutral or anionic complexes was demonstrated. This elegant work has led to the identification of the first metal containing hTK1substrates.
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Scheme 7. Synthesis of thymidine analogs functionalized with regular click ligands at the C3' or N3 position developed as substrates for hTK1. dT = deoxythymidine; A and B represent functional groups for coordination of the 99mTc-ricarbonyl core.
Scheme 7. Synthesis of thymidine analogs functionalized with regular click ligands at the C3' or N3 position developed as substrates for hTK1. dT = deoxythymidine; A and B represent functional groups for coordination of the 99mTc-ricarbonyl core.
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Seridi et al. [53] and Hassanzadeh et al. [54,55] have used CuAAC in the development of brain radiotracers. They describe the conjugation of bi- and tridentate regular click ligands to 1-(2-methoxyphenyl)piperazine (MPP), a pharmacophore frequently found in imaging agents selective for 5-HT1A, a receptor subtype for the neurotransmitter serotonin (5-hydroxytryptamine, 5-HT; Figure 7). The radiotracers exhibited favorable low protein binding, good blood serum stability, and a lipophilicity suitable to cross the blood-brain barrier (BBB). The compound functionalized with a tridentate click ligand was labeled with [99mTc(CO)3]+ and evaluated in vitro and in vivo. Receptor saturation experiments performed with homogenated hippocampus membranes of rats revealed a high receptor affinity (KD) of the compound in the single-digit nanomolar range. In vivo experiments with rats indicated a specific uptake of the radiotracer in the brain, being highest in the hippocampus. Both groups conclude that the 99mTc-labeled MPP derivatives have suitable characteristics for use as central nervous system (CNS) receptor specific imaging agents.
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Figure 7. Bi- and tridentate clicked ligands conjugated to MPP for the development of CNS imaging agents.
Figure 7. Bi- and tridentate clicked ligands conjugated to MPP for the development of CNS imaging agents.
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Finally, implementation of Click-to-Chelate to steroid-based radiotracers is discussed (Figure 8). Dhyani et al. have investigated 99mTc-tricarbonyl-labeled derivatives of 11ß-progesterone and 17α-testosterone for targeting progesterone (PR) and androgen receptors (AR), which are overexpressed in breast and prostate cancer, respectively [56,57]. The compatibility of the site of functionalization of the steroids with regards to biological function as well as stereochemical considerations were taken into account for the design of the radiotracers. While the synthesis of the compounds was straight forward and the stability of the radiometal complexes in biological media was excellent, experiments in vivo using Swiss mice or Wistar rats did not reveal specificities towards the corresponding receptors. These findings may be the result of the bulkiness of the 99mTc chelate in comparison to the steroid vector, which could interfere with the interaction of the compound with its binding site.
Burai et al. have reported the 99mTc-tricarbonyl labeling of tetrahydro-3H-cyclopenta[c]quinoline, the structural design of which was inspired by estrogen steroids (Figure 8) [58]. Derivatives of the parent compound are specific agonists/antagonists of the recently identified G protein-coupled estrogen receptor (GPER), but not the estrogen ERα/ß receptors. GPERs are involved in tumor signaling pathways and have shown promising properties as putatively important biomarkers or therapeutic targets in oncology. Again, receptor specificity of the vector was lost upon functionalization with the radiometal chelate as shown by in vitro assays measuring intracellular calcium immobilization or PI3K activation. In the same work, a series of other 99mTc-labeled derivatives of the parent compound was investigated of which some, particularly those with a spacer moiety between the radiometal chelate and the vector, showed retained activities as GPER agonists/antagonists. Surprisingly, in none of the “clicked” steroid examples discussed above， the effect of a spacer unit between the receptor specific vector and the radiometal chelate has been investigated.
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Figure 8. Examples of steroids or analogs thereof radiolabeled with the 99mTc-tricarbonyl core by Click-to-Chelate.
Figure 8. Examples of steroids or analogs thereof radiolabeled with the 99mTc-tricarbonyl core by Click-to-Chelate.
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8. Summary and Perspectives

Since first reported in 2006, the Click-to-Chelate approach has found numerous applications for the development of radiopharmaceuticals based on the 99mTc-tricarbonyl core. Examples include organometallic radiotracers with potential for applications in nuclear medicine ranging from peptides, vitamins, carbohydrates, steroids to hypoxia imaging agents, substrates for tumor-associated enzymes (e.g. hTK1), and CNS imaging probes. Thus, Click-to-Chelate has had a demonstrated impact on the community involved in radiopharmaceutical sciences.
The majority of applications discussed in this review employ propargyl glycine (Table 1, entry 1) as an alkyne prochelator. This is likely due to its commercial availability and demonstrated equivalency of the resulting tridentate triazolyl ligand system to Nτ-functionalized His. However, other alkyne derivatives (e.g., readily accessible Nα-propargylated amino acid derivatives; Table 1, entry 2) also hold great promise for future applications, especially in terms of the development of multifunctional radioconjugates.
Meanwhile, new and innovative approaches have been described, which expand the portfolio of click chemistry applications in radiopharmaceutical sciences and eventually in nuclear medicine. With respect to radionuclides of technetium and rhenium, the CuAAC has been employed to BFCAs suitable for the complexation of oxo-technetium species [59], the development of a “click-to-cyclize and chelate” strategy for the cyclization of peptides and their radiolabeling [60], and pre-labeling strategies [61] for the labeling of molecules with pre-formed radiometal complexes. The CuAAC technique has also been applied to the preparation of multiple imaging probes derived from the same precursor for a direct comparison of different imaging probes as well imaging modalities [62]. In addition, copper-free click reactions [63] have become increasingly popular for the development of radiotracers [64,65,66,67,68,69] including examples of pre-targeting approaches [70].
Click chemistry, in particular the CuAAC reaction, has demonstrated its high potential for the development of radiotracers. However, there are ample opportunities left for new innovations to be reported in the future and first clinical applications of “clicked” probes are being awaited. Among the many potential applications of click reactions in radiopharmaceutical sciences, Click-to-Chelate will continue to play an important role for the radiolabeling of molecules with the technetium- and rhenium-tricarbonyl cores.

Conflict of Interest

The authors declare no conflicts of interest.

References

  1. Fani, M.; André, J.P.; Mäcke, H.R. 68Ga-PET: a powerful generator-based alternative to cyclotron-based PET radiopharmaceuticals. Contrast Media Mol. Imaging 2008, 3, 53–63. [Google Scholar] [CrossRef]
  2. Amato, I. Nuclear Medicine’s Conundrum. Chem. Eng. News Arch. 2009, 87, 58–64. [Google Scholar] [CrossRef]
  3. National Research Council/National Academy of Science (USA), Medical Isotope Production without Highly Enriched Uranium; National Academies Press: Washington, DC, USA, 2009.
  4. Rösch, F.; Baum, R.P. Generator-based PET radiopharmaceuticals for molecular imaging of tumours: on the way to THERANOSTICS. Dalton Trans. 2011, 40, 6104–6111. [Google Scholar] [CrossRef]
  5. Mindt, T.; Struthers, H.; García-Garayoa, E.; Desbouis, D.; Schibli, R. Strategies for the Development of Novel Tumor Targeting Technetium and Rhenium Radiopharmaceuticals. Chimia 2007, 61, 725–731. [Google Scholar] [CrossRef]
  6. Alberto, R.; Schibli, R.; Egli, A.; Schubiger, A.P.; Abram, U.; Kaden, T.A. A Novel Organometallic Aqua Complex of Technetium for the Labeling of Biomolecules: Synthesis of [99mTc(OH2)3(CO)3]+ from [99mTcO4]– in Aqueous Solution and Its Reaction with a Bifunctional Ligand. J. Am. Chem. Soc. 1998, 120, 7987–7988. [Google Scholar] [CrossRef]
  7. Schibli, R.; la Bella, R.; Alberto, R.; García-Garayoa, E.; Ortner, K.; Abram, U.; Schubiger, P.A. Influence of the Denticity of Ligand Systems on the in Vitro and in Vivo Behavior of 99mTc(I)-Ttricarbonyl Complexes: A Hint for the Future Functionalization of Biomolecules. Bioconjug. Chem. 2000, 11, 345–351. [Google Scholar] [CrossRef]
  8. Alberto, R.; Pak, J.K.; van Staveren, D.; Mundwiler, S.; Benny, P. Mono-, Bi-, or Tridentate Ligands? The Labeling of Peptides with 99mTc-Carbonyls. Biopolymers 2004, 76, 324–333. [Google Scholar] [CrossRef]
  9. Valliant, J.F.; Morel, P.; Schaffer, P.; Kaldis, J.H. Carboranes as Ligands for the Preparation of Organometallic Tc and Re Radiopharmaceuticals. Synthesis of [M(CO)3(η5-2,3-C2B9H11)]− and rac-[M(CO)3(η5-2-R-2,3-C2B9H11)]– (M = Re, 99Tc; R = CH2CH2CO2H) from [M(CO)3Br3]2−. Inorg. Chem. 2002, 41, 628–630. [Google Scholar] [CrossRef]
  10. Liu, S. The role of coordination chemistry in the development of target-specific radiopharmaceuticals. Chem. Soc. Rev. 2004, 33, 445–461. [Google Scholar] [CrossRef]
  11. Kolb, H.C.; Finn, M.G.; Sharpless, K.B. Click Chemistry: Diverse Chemical Function from a Few Good Reactions. Angew. Chem. Int. Ed. 2001, 40, 2004–2021. [Google Scholar] [CrossRef]
  12. Rostovtsev, V.V.; Green, L.G.; Fokin, V.V.; Sharpless, K.B. A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Regioselective “Ligation” of Azides and Terminal Alkynes. Angew. Chem. Int. Ed. Engl. 2002, 41, 2596–2599. [Google Scholar] [CrossRef]
  13. Tornøe, C.W.; Christensen, C.; Meldal, M. Peptidotriazoles on Solid Phase: [1,2,3]-Triazoles by Regiospecific Copper(I)-Catalyzed 1,3-Dipolar Cycloadditions of Terminal Alkynes to Azides. J. Org. Chem. 2002, 67, 3057–3064. [Google Scholar] [CrossRef]
  14. Meldal, M.; Tornøe, C.W. Cu-Catalyzed Azide-Alkyne Cycloaddition. Chem. Rev. 2008, 108, 2952–3015. [Google Scholar] [CrossRef]
  15. Finn, M.G.; Fokin, V.V. Click chemistry: Function follows form (Special Issue). Chem. Soc. Rev. 2010, 39, 1221–1408. [Google Scholar] [CrossRef]
  16. Hein, C.D.; Liu, X.-M.; Wang, D. Click chemistry, a powerful tool for pharmaceutical sciences. Pharm. Res. 2008, 25, 2216–2230. [Google Scholar] [CrossRef]
  17. Kolb, H.C.; Walsh, J.C.; Chen, K. Click chemistry method for synthesizing molecular imaging probes. WO2006116629(A2), 2 November 2006. [Google Scholar]
  18. Marik, J.; Sutcliffe, J.L. Click for PET: rapid preparation of [18F]fluoropeptides using CuI catalyzed 1,3-dipolar cycloaddition. Tetrahedron Lett. 2006, 47, 6681–6684. [Google Scholar] [CrossRef]
  19. Mamat, C.; Ramenda, T.; Wuest, F.R. Recent Applications of Click Chemistry for the Synthesis of Radiotracers for Molecular Imaging. Mini Rev. Org. Chem. 2009, 6, 21–34. [Google Scholar] [CrossRef]
  20. Wängler, C.; Schirrmacher, R.; Bartenstein, P.; Wängler, B. Click-Chemistry Reactions in Radiopharmaceutical Chemistry: Fast & Easy Introduction of Radiolabels into Biomolecules for In Vivo Imaging. Curr. Med. Chem. 2010, 17, 1092–1116. [Google Scholar] [CrossRef]
  21. Mindt, T.L.; Struthers, H.; Brans, L.; Anguelov, T.; Schweinsberg, C.; Maes, V.; Tourwé, D.; Schibli, R. “Click to Chelate”: Synthesis and Installation of Metal Chelates into Biomolecules in a Single Step. J. Am. Chem. Soc. 2006, 128, 15096–15097. [Google Scholar] [CrossRef]
  22. Struthers, H.; Mindt, T.L.; Schibli, R. Metal chelating systems synthesized using the copper(I) catalyzed azide-alkyne cycloaddition. Dalton Trans. 2010, 39, 675–696. [Google Scholar] [CrossRef]
  23. Van Kasteren, S.I.; Kramer, H.B.; Jensen, H.H.; Campbell, S.J.; Kirkpatrick, J.; Oldham, N.J.; Anthony, D.C.; Davis, B.G. Expanding the diversity of chemical protein modification allows post-translational mimicry. Nature 2007, 446, 1105–1109. [Google Scholar] [CrossRef]
  24. Beatty, K.E.; Liu, J.C.; Xie, F.; Dieterich, D.C.; Schuman, E.M.; Wang, Q.; Tirrell, D.A. Fluorescence Visualization of Newly Synthesized Proteins in Mammalian Cells. Angew. Chem. Int. Ed. Engl. 2006, 45, 7364–7367. [Google Scholar] [CrossRef]
  25. Ohta, A.; Yamagishi, Y.; Suga, H. Synthesis of biopolymers using genetic code reprogramming. Curr. Opin. Chem. Biol. 2008, 12, 159–167. [Google Scholar] [CrossRef]
  26. Wang, L.; Schultz, P.G. Expanding the Genetic Code. Angew. Chem. Int. Ed. 2005, 44, 34–66. [Google Scholar] [CrossRef]
  27. Baskin, J.M.; Bertozzi, C.R. Bioorthogonal Click Chemistry: Covalent Labeling in Living Systems. QSAR Comb. Sci. 2007, 26, 1211–1219. [Google Scholar] [CrossRef]
  28. Pak, J.K.; Benny, P.; Spingler, B.; Ortner, K.; Alberto, R. Nε Functionalization of Metal and Organic Protected L-Histidine for a Highly Efficient, Direct Labeling of Biomolecules with [Tc(OH2)3(CO)3]+. Chem. Eur. J. 2003, 9, 2053–2061. [Google Scholar] [CrossRef]
  29. Brans, L.; García-Garayoa, E.; Schweinsberg, C.; Maes, V.; Struthers, H.; Schibli, R.; Tourwé, D. Synthesis and Evaluation of Bombesin Analogues Conjugated to Two Different Triazolyl-Derived Chelators for 99mTc Labeling. ChemMedChem 2010, 5, 1717–1725. [Google Scholar] [CrossRef]
  30. Anderson, C.B.; Elliott, A.B.S.; Lewis, J.E.M.; McAdam, C.J.; Gordon, K.C.; Crowley, J.D. fac-Re(CO)3 complexes of 2,6-bis(4-substituted-1,2,3-triazol-1-ylmethyl)pyridine"click" ligands: synthesis, characterisation and photophysical properties. Dalton Trans. 2012, 41, 14625–14632. [Google Scholar] [CrossRef]
  31. Urankar, D.; Pinter, B.; Pevec, A.; de Proft, F.; Turel, I.; Košmrlj, J. Click-Triazole N2 Coordination to Transition-Metal Ions Is Assisted by a Pendant Pyridine Substituent. Inorg. Chem. 2010, 49, 4820–4829. [Google Scholar] [CrossRef]
  32. Alberto, R.; Egli, A.; Abram, U.; Hegetschweiler, K.; Gramlich, V.; Schubiger, P.A. Synthesis and Reactivity of [NEt4]2[ReBr3(CO)3]. Formation and Structural Characterization of the Clusters [NEt4][Re3(µ3-OH)(µ-OH)3(CO)9] and [NEt4][Re2(µ-OH)3(CO)6] by Alkaline Titration. J. Chem. Soc. Dalton Trans. 1994, 1, 2815–2820. [Google Scholar]
  33. Struthers, H.; Hagenbach, A.; Abram, U.; Schibli, R. Organometallic [Re(CO)3]+ and [Re(CO)2(NO)]2+ Labeled Substrates for Human Thymidine Kinase 1. Inorg. Chem. 2009, 48, 5154–5163. [Google Scholar] [CrossRef]
  34. Mindt, T.L.; Struthers, H.; Spingler, B.; Brans, L.; Tourwé, D.; García-Garayoa, E.; Schibli, R. Molecular Assembly of Multifunctional 99mTc Radiopharmaceuticals Using “Clickable”Amino Acid Derivatives. ChemMedChem. 2010, 5, 2026–2038. [Google Scholar] [CrossRef]
  35. Struthers, H.; Spingler, B.; Mindt, T.L.; Schibli, R. “Click-to-Chelate”: Design and Incorporation of Triazole-Containing Metal-Chelating Systems into Biomolecules of Diagnostic and Therapeutic Interest. Chem. Eur. J. 2008, 14, 6173–6183. [Google Scholar] [CrossRef]
  36. Mindt, T.L.; Schweinsberg, C.; Brans, L.; Hagenbach, A.; Abram, U.; Tourwé, D.; García-Garayoa, E.; Schibli, R. A Click Approach to Structurally Diverse Conjugates Containing a Central Di-1,2,3-triazole Metal Chelate. ChemMedChem 2009, 4, 529–539. [Google Scholar] [CrossRef]
  37. Wang, C.; Zhou, W.; Yu, J.; Zhang, L.; Wang, N. A study of the radiosynthesis of fac-[188Re(CO)3(H2O)3]+ and its application in labeling 1,2,3-triazole analogs obtained by click chemistry. Nucl. Med. Commun. 2012, 33, 84–89. [Google Scholar] [CrossRef]
  38. Mindt, T.L.; Müller, C.; Melis, M.; de Jong, M.; Schibli, R. “Click-to-Chelate”: In Vitro and In Vivo Comparison of a 99mTc(CO)3-Labeled N(τ)-Histidine Folate Derivative with Its Isostructural, Clicked 1,2,3-Triazole Analogue. Bioconjug. Chem. 2008, 19, 1689–1695. [Google Scholar] [CrossRef]
  39. Zhang, X.; Cabral, P.; Bates, M.; Gambini, J.P.; Fernandez, M.; Calzada, V.; Gallazzi, F.; Larimer, B.; Figueroa, S.D.; Alonso, O.; et al. In Vitro and In Vivo Evaluation of [99mTc(CO)3]-Radiolabeled ErbB-2-Targeting Peptides for Breast Carcinoma Imaging. Curr. Radiopharm. 2010, 3, 308–321. [Google Scholar] [CrossRef]
  40. Müller, C.; Schibli, R. Folic Acid Conjugates for Nuclear Imaging of Folate Receptor-Positive Cancer. J. Nucl. Med. 2011, 52, 1–4. [Google Scholar] [CrossRef]
  41. Sancho, V.; di Florio, A.; Moody, T.W.; Jensen, R.T. Bombesin receptor-mediated imaging and cytotoxicity: review and current status. Curr. Drug. Deliv. 2011, 8, 79–134. [Google Scholar] [CrossRef]
  42. Ferro-Flores, G.; Rivero, I.A.; Santos-Cuevas, C.L.; Sarmiento, J.I.; Arteaga de Murphy, C.; Ocampo-García, B.E.; García-Becerra, R.; Ordaz-Rosado, D. Click chemistry for [99mTc (CO)3] labeling of Lys3-bombesin. Appl. Radiat. Isotopes 2010, 68, 2274–2278. [Google Scholar] [CrossRef]
  43. Liu, S. 6-Hydrazinonicotinamide Derivatives as Bifunctional Coupling Agents for 99mTc-Labeling of Small Biomolecules. Top. Curr. Chem. 2005, 252, 117–153. [Google Scholar]
  44. Sparr, C.; Michel, U.; Marti, R.E.; Müller, C.; Schibli, R.; Moser, R.; Groehn, V. Synthesis of a Novel γ-Folic Acid-Nτ-Histidine Conjugate Suitable for Labeling with 99mTc and 188Re. Synthesis 2009, 5, 787–792. [Google Scholar]
  45. Kluba, C.A.; Bauman, A.; Valverde, I.E.; Vomstein, S.; Mindt, T.L. Dual-targeting conjugates designed to improve the efficacy of radiolabeled peptides. Org. Biomol. Chem. 2012, 10, 7594–7602. [Google Scholar] [CrossRef]
  46. Kim, E.-M.; Joung, M.-H.; Lee, C.-M.; Jeong, H.-J.; Lim, S.T.; Sohn, M.-H.; Kim, D.W. Synthesis of Tc-99m labeled 1,2,3-triazole-4-yl c-met binding peptide as a potential c-met receptor kinase positive tumor imaging agent. Bioorg. Med. Chem. Lett. 2010, 20, 4240–4243. [Google Scholar] [CrossRef]
  47. Pauwels, E.K.J.; Sturm, E.J.C.; Bombardieri, E.; Cleton, F.J.; Stokkel, M.P.M. Positron-emission tomography with [18F]fluorodeoxyglucose. Part I. Biochemical uptake mechanism and its implication for clinical studies. J. Cancer Res. Clin. Oncol. 2000, 126, 549–559. [Google Scholar] [CrossRef]
  48. Dumas, C.; Petrig, J.; Frei, L.; Spingler, B.; Schibli, R. Functionalization of Glucose at Position C-3 for Transition Metal Coordination: Organo-Rhenium Complexes with Carbohydrate Skeletons. Bioconjug. Chem. 2005, 16, 421–428. [Google Scholar] [CrossRef]
  49. Fernández, S.; Crócamo, N.; Incerti, M.; Giglio, J.; Scarone, L.; Rey, A. Preparation and preliminary bioevaluation of a 99mTc(CO)3-glucose derivative prepared by a click chemistry route. J. Label. Compd. Radiopharm. 2012, 55, 274–280. [Google Scholar] [CrossRef]
  50. Kizaka-Kondoh, S.; Konse-Nagasawa, H. Significance of nitroimidazole compounds and hypoxia-inducible factor-1 for imaging tumor hypoxia. Cancer Sci. 2009, 100, 1366–1373. [Google Scholar] [CrossRef]
  51. Fernández, S.; Giglio, J.; Rey, A.M.; Cerecetto, H. Influence of ligand denticity on the properties of novel 99mTc-(I)-carbonyl complexes. Application to the development of radiopharmaceuticals for imaging hypoxic tissue. Bioorg. Med. Chem. 2012, 20, 4040–4048. [Google Scholar] [CrossRef]
  52. Struthers, H.; Viertl, D.; Kosinski, M.; Spingler, B.; Buchegger, F.; Schibli, R. Charge Dependent Substrate Activity of C3′ and N3 Functionalized, Organometallic Technetium and Rhenium-LabeledThymidine Derivatives toward Human Thymidine Kinase 1. Bioconjug. Chem. 2010, 21, 622–634. [Google Scholar] [CrossRef] [Green Version]
  53. Seridi, A.; Wolff, M.; Boulay, A.; Saffon, N.; Coulais, Y.; Picard, C.; Machura, B.; Benoist, E. Rhenium(I) and technetium(I) complexes of a novel pyridyltriazole-based ligand containing an arylpiperazine pharmacophore: Synthesis, crystal structures, computational studies and radiochemistry. Inorg. Chem. Commun. 2011, 14, 238–242. [Google Scholar] [CrossRef]
  54. Hassanzadeh, L.; Erfani, M.; Ebrahimi, S.E.S. 2-Amino-3-(1-(4-(4-(2-methoxyphenyl) piperazine-1-yl)butyl)-1H-1,2,3-triazol-4-yl) propanoic acid: synthesized, 99mTc-tricarbonyl labeled, and bioevaluated as a potential 5HT1A receptor ligand. J. Label. Compd. Radiopharm. 2012, 55, 371–376. [Google Scholar] [CrossRef]
  55. Hassanzadeh, L.; Erfani, M.; Najafi, R.; Shafiei, M.; Amini, M.; Shafiee, A.; Ebrahimi, S.E.S. Synthesis, radiolabeling and bioevaluation of a novel arylpiperazine derivative containing triazole as a 5-HT1A receptor imaging agents. Nucl. Med. Biol. 2013, 40, 227–232. [Google Scholar] [CrossRef]
  56. Dhyani, M.V.; Satpati, D.; Korde, A.; Sarma, H.D.; Kumar, C.; Banerjee, S. Preparation and preliminary bioevaluation of 99mTc(CO)3-11ß-progesterone derivative prepared via click chemistry route. Nucl. Med. Biol. 2010, 37, 997–1004. [Google Scholar] [CrossRef]
  57. Dhyani, M.V.; Satpati, D.; Korde, A.; Banerjee, S. Synthesis and Preliminary Bioevaluation of 99mTc(CO)3-17α-Triazolylandrost-4-Ene-3-One Derivative Prepared via Click Chemistry Route. Cancer Biother. Radio. 2011, 26(5), 539–545. [Google Scholar] [CrossRef]
  58. Burai, R.; Ramesh, C.; Nayak, T.K.; Dennis, M.K.; Bryant, B.K.; Prossnitz, E.R.; Arterburn, J.B. Synthesis and Characterization of Tricarbonyl-Re/Tc(I) Chelate Probes Targeting the G Protein-Coupled Estrogen Receptor GPER/GPR30. PLoS One 2012, 7, e46861. [Google Scholar]
  59. Martinage, O.; Le Clainche, L.; Czarny, B.; Dugave, C. Synthesis and biological evaluation of a new triazole-oxotechnetium complex. Org. Biomol. Chem. 2012, 10, 6484–6490. [Google Scholar] [CrossRef]
  60. Simpson, E.J.; Hickey, J.L.; Luyt, L.G. Click to Cyclize and Chelate. J. Label. Compd. Radiopharm 2011, 54, S350–S350. [Google Scholar]
  61. Moore, A.L.; Bučar, D.-K.; MacGillivray, L.R.; Benny, P.D. “Click” labeling strategy for M(CO)3 (M = Re, 99mTc) prostate cancer targeted Flutamide agents. Dalton Trans. 2010, 39, 1926–1928. [Google Scholar] [CrossRef]
  62. Mindt, T.L.; Müller, C.; Stuker, F.; Salazar, J.-F.; Hohn, A.; Mueggler, T.; Rudin, M.; Schibli, R. A “Click Chemistry” Approach to the Efficient Synthesis of Multiple Imaging Probes Derived from a Single Precursor. Bioconjug. Chem. 2009, 20, 1940–1949. [Google Scholar] [CrossRef]
  63. Jewett, J.C.; Bertozzi, C.R. Cu-free click cycloaddition reactions in chemical biology. Chem. Soc. Rev. 2010, 39, 1272–1279. [Google Scholar] [CrossRef]
  64. Van Berkel, S.S.; Dirks, A.J.; Meeuwissen, S.A.; Pingen, D.L.L.; Boerman, O.C.; Laverman, P.; van Delft, F.L.; Cornelissen, J.J.L.M.; Rutjes, F.P.J.T. Application of Metal-Free Triazole Formation in the Synthesis of Cyclic RGD-DTPA Conjugates. ChemBioChem 2008, 9, 1805–1815. [Google Scholar] [CrossRef]
  65. Campbell-Verduyn, L.S.; Mirfeizi, L.; Schoonen, A.K.; Dierckx, R.A.; Elsinga, P.H.; Feringa, B.L. Strain-Promoted Copper-Free “Click” Chemistry for 18F Radiolabeling of Bombesin. Angew. Chem. Int. Ed. Engl. 2011, 50, 11117–11120. [Google Scholar]
  66. Bouvet, V.; Wuest, M.; Wuest, F. Copper-free click chemistry with the short-lived positron emitter fluorine-18. Org. Biomol. Chem. 2011, 9, 7393–7399. [Google Scholar] [CrossRef]
  67. Zeglis, B.M.; Mohindra, P.; Weissmann, G.I.; Divilov, V.; Hilderbrand, S.A.; Weissleder, R.; Lewis, J.S. Modular Strategy for the Construction of Radiometalated Antibodies for Positron Emission Tomography Based on Inverse Electron Demand Diels-Alder Click Chemistry. Bioconjug. Chem. 2011, 22, 2048–2059. [Google Scholar] [CrossRef]
  68. Chen, K.; Wang, X.; Lin, W.-Y.; Shen, C.K.F.; Yap, L.-P.; Hughes, L.D.; Conti, P.S. Strain-Promoted Catalyst-Free Click Chemistry for Rapid Construction of 64Cu-Labeled PET Imaging Probes. ACS Med. Chem. Lett. 2012, 3, 1019–1023. [Google Scholar] [CrossRef]
  69. Devaraj, N.K.; Weissleder, R. Biomedical Applications of Tetrazine Cycloadditions. Accounts Chem. Res. 2011, 44, 816–827. [Google Scholar] [CrossRef]
  70. Rossin, R.; Verkerk, P.R.; van den Bosch, S.M.; Vulders, R.C.M.; Verel, I.; Lub, J.; Robillard, M.S. In Vivo Chemistry for Pretargeted Tumor Imaging in Live Mice. Angew. Chem. Int. Ed. Engl. 2010, 49, 3375–3378. [Google Scholar]

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MDPI and ACS Style

Kluba, C.A.; Mindt, T.L. Click-to-Chelate: Development of Technetium and Rhenium-Tricarbonyl Labeled Radiopharmaceuticals. Molecules 2013, 18, 3206-3226. https://doi.org/10.3390/molecules18033206

AMA Style

Kluba CA, Mindt TL. Click-to-Chelate: Development of Technetium and Rhenium-Tricarbonyl Labeled Radiopharmaceuticals. Molecules. 2013; 18(3):3206-3226. https://doi.org/10.3390/molecules18033206

Chicago/Turabian Style

Kluba, Christiane A., and Thomas L. Mindt. 2013. "Click-to-Chelate: Development of Technetium and Rhenium-Tricarbonyl Labeled Radiopharmaceuticals" Molecules 18, no. 3: 3206-3226. https://doi.org/10.3390/molecules18033206

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