Hepatotoxicity Evaluation of Aqueous Extract from Scutia buxifolia
Abstract
:1. Introduction
2. Results
2.1. Quantification of Phenolic and Flavonoids Compounds by HPLC-DAD
Component | Extract | |
---|---|---|
mg/g * | % | |
Gallic acid | 51.88 ± 0.01 a | 5.18 |
Chlorogenic acid | 3.98 ± 0.19 b | 0.39 |
Caffeic acid | 247.21 ± 2.17 c | 24.72 |
Rutin | 11.36 ± 0.63 d | 11.16 |
Quercetin | 132.59 ± 12.83 e | 13.25 |
Kaempferol | 1.75 ± 0.01 f | 0.17 |
2.2. Biochemical Parameters in Blood
2.3. Effect of S. buxifolia on Lipid Peroxidation
2.4. Effect of S. buxifolia on Tissue Sulfhydryl Groups (NPSH)
2.5. Effect of S. Buxifolia on Enzymatic Antioxidant Defense
2.6. Histopathological Studies
3. Discussion
4. Experimental
4.1. Plant Material and Extract Preparation
4.2. Chemicals
4.3. Quantification of Phenolics and Flavonoids Compounds by HPLC-DAD
4.4. Animals
4.5. Experimental Design
- Group I: Received distilled water (0 mg of SBSB/kg body weight).
- Group II: Received SBSB at a concentration of 100 mg/kg body weight.
- Group III: Received SBSB at a concentration of 200 mg/kg body weight.
- Group IV: Received SBSB at a concentration of 400 mg/kg body weight.
4.6. Liver Samples
4.7. Measurement of Biochemical Parameters in Blood
4.8. Thiobarbituric Acid Reactive Substances
4.9. Non-Enzimatic Antioxidant Defense
4.10. Catalase Activity
4.11. Superoxide Dismutase Activity
4.12. Histopathological Studies
4.13. Statistical Analysis
5. Conclusions
Acknowledgments
Conflicts of Interest
References
- Herrera, S.; Bruguera, M. Hepatotoxidad inducida por el uso de hierbas y medicamentos para perder peso. Gastroenterol. Hepatol. 2008, 31, 447–453. [Google Scholar] [CrossRef]
- Beyerstein, B. Alternative medicine and common errors of reasoning. Acad. Med. 2001, 76, 230–237. [Google Scholar] [CrossRef]
- Zhou, S.; Koh, H.L.; Gao, Y.; Gong, Z.Y.; Lee, E.J. Herbal bioactivation: The good, the bad and the ugly. Life Sci. 2004, 74, 935–968. [Google Scholar] [CrossRef]
- Chojkier, M. Hepatic sinusoidal-obstruction syndrome: Toxicity of pyrrolizidine alkaloids. J. Hepatol. 2003, 39, 437–446. [Google Scholar] [CrossRef]
- Corns, C.M. Herbal remedies and clinical biochemistry. Ann. Clin. Biochem. 2003, 40, 489–507. [Google Scholar] [CrossRef]
- Rodrigues, G.; Marcolin, É.; Bona, S.; Porawski, M.; Lehmann, M.; Marroni, N.P. Hepatics alterations and genotoxic effects of Croton cajucara Benth (sacaca) in diabetic rats. Arq. Gastrenterol. 2010, 47, 301–305. [Google Scholar] [CrossRef]
- Furbee, R.B.; Barlotta, K.S.; Allen, M.K.; Holstege, C.P. Hepatotoxicity associated with herbal products. Clin. Lab. Med. 2006, 26, 227–241. [Google Scholar] [CrossRef]
- Steenkamp, V.; Stewart, M.J.; Zuckerman, M. Clinical and analytic aspects of pyrrolizidine poisoning caused by South African traditional medicines. Ther. Drug Monit. 2000, 22, 302–306. [Google Scholar] [CrossRef]
- DeLeve, L.D.; Shulman, H.M.; McDonald, G.B. Toxic injury to hepatic sinusoids: Sinusoidal obstruction syndrome (veno-occlusive disease). Semin. Liver Dis. 2002, 22, 27–42. [Google Scholar] [CrossRef]
- McDermott, W.V.; Ridker, P.M. The Budd-Chiari syndrome and hepatic venoocclusive disease.Recognition and treatment. Arch. Surg. 1990, 125, 525–527. [Google Scholar] [CrossRef]
- Wasicky, R.; Wasicky, M.; Joachimovits, R. Erstuntersuchungen na Coronilha-Scutia buxifolia Reissek. Planta Med. 1964, 12, 13–25. [Google Scholar] [CrossRef]
- Boligon, A.A.; Brum, T.F.; Frohlich, J.K.; Froeder, A.L.F.; Athayde, M.L. HPLC/DAD profile and determination of total phenolics, flanovonoids, tannins and alkaloids contents of Scutia buxifolia Reissek stem bark. Res. J. Phytochem. 2012, 6, 84–91. [Google Scholar] [CrossRef]
- Boligon, A.A.; Magoga, B.R.; Feltrin, A.C.; Janovik, V.; Athayde, M.L. Potencial antioxidante in vitro, conteúdo de fenóis e flavonóides nos ramos de Scutia buxifolia Reiss. Saúde 2009, 35, 34–38. [Google Scholar]
- Boligon, A.A.; Pereira, R.P.; Feltrin, A.C.; Machado, M.M.; Janovik, V.; Rocha, J.B.; Athayde, M.L. Antioxidant activities of flavonol derivatives from the leaves and stem bark of Scutia buxifolia Reiss. Bioresour. Technol. 2009, 100, 6592–6598. [Google Scholar]
- Morel, A.F.; Maldaner, G.; Ilha, V.; Missau, F.; Silva, U.F.; Dalcol, I.I. Cyclopeptide alkaloids from Scutia buxifolia Reiss and their antimicrobial activity. Phytochem. 2005, 72, 2571–2576. [Google Scholar]
- Trevisan, G.; Maldaner, G.; Velloso, N.A.; da Silva Sant'Anna, G.; Ilha, V.; de Campos Velho Gewehr, C.; Rubin, M.A.; Morel, A.F.; Ferreira, J. Antinociceptive effects of 14-membered cyclopeptide alkaloid. J. Nat. Prod. 2009, 72, 608–612. [Google Scholar] [CrossRef]
- Boligon, A.A.; Janovik, V.; Frohlich, J.K.; Spader, T.B.; Froeder, A.L.; Alves, S.H.; Athayde, M.L. Antimicrobial and cytotoxic activities of leaves, twigs and stem bark of Scutia buxifolia Reissek. Nat Prod. Res. 2012, 26, 939–944. [Google Scholar]
- Freitas, R.B.; da Costa Araldi, I.C.; Boligon, A.A.; de Brum, T.F.; Rovani, B.T.; Piana, M.; Zadra, M.; Athayde, M.L.; de Freitas Bauermann, L. Phytochemical analysis and toxicity investigation of stem bark of Reissek. Nat. Prod. Res. 2012, 1, 1–5. [Google Scholar]
- Dickel, M.L.; Rates, S.M.K.; Ritter, M.R. Plants popularly used for losing weight purposes in Porto Alegre, South Brazil. J. Ethnopharmacol. 2007, 19, 60–71. [Google Scholar] [CrossRef]
- Boligon, A.A.; Feltrin, A.C.; Machado, M.M.; Janovik, V.; Athayde, M.L. HPLC Analysis and phytoconstituents isolated from ethyl acetate fraction of Scutia buxifolia reiss. leaves. Lat. Am. J. Pharm. 2009, 1, 121–124. [Google Scholar]
- Yang, E.J.; Kim, S.I.; Park, S.Y.; Bang, H.Y.; Jeong, J.H.; So, J.H.; Rhee, I.K.; Song, K.S. Fermentation enhances the in vitro antioxidative effect of onion (Allium cepa) via an increase in quercetin content. Food Chem. Toxicol. 2012, 50, 2042–2048. [Google Scholar]
- Al-Habori, M.; Al-Aghbari, A.; Al-Mamary, M.; Baker, M. Toxicological evaluation of Catha edulis leaves: A long term feeding experiment in animals. J. Ethnopharmacol. 2002, 83, 209–217. [Google Scholar] [CrossRef]
- Cohen, J.A.; Kaplan, M.M. The SGOT/SGPT ratio an indicador of alcoholic liver disease. Dig. Dis. Sci. 1979, 24, 835–838. [Google Scholar] [CrossRef]
- Santosh, N.; Mohan, K.; Royana, S.; Yamini, T.B. Hepatotoxicity of tuber of Indian Kudzu (Pueraria tuberosa) in rats. Food Chem. Toxicol. 2010, 40, 1066–1071. [Google Scholar]
- Barcelos, G.R.; Angeli, J.P.; Serpeloni, J.M.; Grotto, D.; Rocha, B.A.; Bastos, J.K.; Knasmüller, S.; Júnior, F.B. Quercetin protects human-derived liver cells against mercury-induced DNA-damage and alterations of the redox status. Mutat. Res. 2011, 726, 109–115. [Google Scholar] [CrossRef]
- Moran, L.K.; Gutteridge, J.M.C.; Quinlan, G.J. Thiols in cellular redox signaling and control. Curr. Med. Chem. 2001, 8, 763–772. [Google Scholar] [CrossRef]
- Dickinson, D.A.; Forman, H.J. Cellular glutathione and thiols metabolism. Biochem. Pharmacol. 2002, 64, 1019–1026. [Google Scholar] [CrossRef]
- Halliwell, B.; Gutteridge, J.C. Free Radicals in Biology and Medicine; Oxford University Press: New York, NY, USA, 2007. [Google Scholar]
- Bhor, V.M.; Raghuram, N.; Sivakami, S. Oxidative damage and altered antioxidant enzyme activities in the small intestine of streptozotocin-induced diabetic rats. Int. J. Biochem. Cell Biol. 2004, 36, 89–97. [Google Scholar] [CrossRef]
- McCuskey, R.S.; Nishida, J.; Eguchi, H.; McDonnell, D.; Baker, G.L.; Ekataksin, W.; Krasovich, M.A.; Rudi, V.; Seitz, H.K.; Urbaschek, B.; et al. Role of endotoxin in the hepatic microvascular inflammatory response to ethanol. J. Gastroenterol. Hepatol. 1995, 10, S18–S23. [Google Scholar] [CrossRef]
- Wang, J.F.; Komarov, P; de Groot, H. Luminol chemiluminescence in rat macrophages and granulocytes: The role of NO, O2/H2O2, and HOCl. Arch. Biochem. Biophys. 1993, 304, 189–196. [Google Scholar] [CrossRef]
- Remirez, D.; Fernández, V.; Tapia, G.; González, R.; Videla, L.A. Influence of Cphycocyanin on hepatocellular parameters related to liver oxidative stress, Kupffer cell functioning. Inflamm. Res. 2002, 51, 351–356. [Google Scholar] [CrossRef]
- Spitzer, J.A.; Zheng, M.; Kolls, J.K.; Vande Stouwe, C.; Spitzer, J.J. Ethanol and LPS modulate NF-kappaB activation, inducible NO synthase, COX-2 gene expression in rat liver cells in vivo. Front. Biosci. 2002, 7, a99–a108. [Google Scholar] [CrossRef]
- Blazka, M.E.; Wilmer, J.L.; Holladay, S.D.; Wilson, R.E.; Luster, M.I. Role of proinflammatory cytokines in acetaminophen hepatotoxicity. Toxicol. Appl. Pharmacol. 1995, 133, 43–52. [Google Scholar] [CrossRef]
- Evaristo, L.M.; Leitão, M.C. Identificação e quantificação por DAD-HPLC da fracção fenólica contida em folhar de Quercus suber L. Silva Lusitana 2001, 9, 135–141. [Google Scholar]
- Waynforth, P.L.H.B. Experimental and Surgical Technique in the Rat; Academic Press: London, UK, 1980. [Google Scholar]
- Buege, J.A.; Aust, S.D. Microsomal peroxidation. Methods Enzymol. 1987, 52, 302–310. [Google Scholar]
- Ellman, G.L. Tissue sulfhydryl groups. Arch. Biochem. Biophys. 1959, 82, 70–77. [Google Scholar] [CrossRef]
- Aebi, H. Catalase in vitro. Methods Enzymol. 1984, 105, 121–126. [Google Scholar]
- Boveris, A.; Chance, B. The mitochondrial generation of hydrogen peroxide: General properties and effect of hyperbaric oxygen. Biochem. J. 1973, 134, 707–716. [Google Scholar]
- Mota, C.S.; Freitas, R.B.; Athayde, M.L.; Boligon, A.A.; Augusti, P.R.; Somacal, S.; Rocha, M.P.; Bauermann, L.F. Effect of Vernonia cognate on oxidative damage induced by ethanol in rats. Human Exp. Toxicol. 2011, 30, 675–684. [Google Scholar] [CrossRef]
- Sample Availability: Samples of the plant and extract are available from the authors.
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De Freitas, R.B.; Rovani, B.T.; Boligon, A.A.; De Brum, T.F.; Piana, M.; Da Silva Jesus, R.; Veloso, C.F.; Kober, H.; Moresco, R.N.; Da Costa Araldi, I.C.; et al. Hepatotoxicity Evaluation of Aqueous Extract from Scutia buxifolia. Molecules 2013, 18, 7570-7583. https://doi.org/10.3390/molecules18077570
De Freitas RB, Rovani BT, Boligon AA, De Brum TF, Piana M, Da Silva Jesus R, Veloso CF, Kober H, Moresco RN, Da Costa Araldi IC, et al. Hepatotoxicity Evaluation of Aqueous Extract from Scutia buxifolia. Molecules. 2013; 18(7):7570-7583. https://doi.org/10.3390/molecules18077570
Chicago/Turabian StyleDe Freitas, Robson Borba, Bruno Tomazele Rovani, Aline Augusti Boligon, Thiele Faccim De Brum, Mariana Piana, Roberta Da Silva Jesus, Carolina Fantinel Veloso, Helena Kober, Rafael Noal Moresco, Isabel Cristina Da Costa Araldi, and et al. 2013. "Hepatotoxicity Evaluation of Aqueous Extract from Scutia buxifolia" Molecules 18, no. 7: 7570-7583. https://doi.org/10.3390/molecules18077570