Isolation and Synthesis of a Bioactive Benzenoid Derivative from the Fruiting Bodies of Antrodia camphorata
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Identification
Position | Antrocamphin O (1) | ||
---|---|---|---|
δC | δH (J in Hz) | HMBC | |
1 | 136.3 | ||
2 | 139.5 | ||
3 | 137.2 | ||
4 | 127.8 | ||
5 | 109.9 | ||
6 | 139.8 | ||
1′ | 83.5 | ||
2′ | 97.5 | ||
3′ | 127.2 | ||
4′ | 121.0 | 5.24 (br s), 5.35 (br s) | 2′, 3′, 5′ |
5′ | 23.6 | 1.98 (br s) | 2′,3′, 4′ |
6′ | 101.4 | 5.91 (s) | 1, 2 |
3-OMe | 60.0 | 3.85 (s) | 3 |
6-OMe | 60.3 | 3.96 (s) | 6 |
4-Me | 13.8 | 2.25 (s) | 3, 4, 5 |
2.2. Synthesis and Structural Characterization of Antrocamphin O (1)
2.3. Cytotoxicity Assay
Compound | IC50 (µM) | |||
---|---|---|---|---|
HT29 | HTC15 | DLD-1 | Colo205 | |
1 | >100 | 70 | >100 | 9.3 |
2 | >100 | >100 | >100 | 43.4 |
3 | 14 | 39 | 40 | >100 |
5-FU | 11.2 | 3.1 | 9.5 | 17 |
3. Experimental
3.1. General Methods
3.2. Plant Material
3.3. Extraction and Isolation
3.4. Synthesis
3.5. In Vitro Cytotoxicity Assay
3.5.1. Cell Culture
3.5.2. Cytotoxicity Assay
3.5.3. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Chen, P.-Y.; Wu, J.-D.; Tang, K.-Y.; Yu, C.-C.; Kuo, Y.-H.; Zhong, W.-B.; Lee, C.-K. Isolation and Synthesis of a Bioactive Benzenoid Derivative from the Fruiting Bodies of Antrodia camphorata . Molecules 2013, 18, 7600-7608. https://doi.org/10.3390/molecules18077600
Chen P-Y, Wu J-D, Tang K-Y, Yu C-C, Kuo Y-H, Zhong W-B, Lee C-K. Isolation and Synthesis of a Bioactive Benzenoid Derivative from the Fruiting Bodies of Antrodia camphorata . Molecules. 2013; 18(7):7600-7608. https://doi.org/10.3390/molecules18077600
Chicago/Turabian StyleChen, Pi-Yu, Jen-Der Wu, Kai-Yih Tang, Chieh-Chou Yu, Yueh-Hsiung Kuo, Wen-Bin Zhong, and Ching-Kuo Lee. 2013. "Isolation and Synthesis of a Bioactive Benzenoid Derivative from the Fruiting Bodies of Antrodia camphorata " Molecules 18, no. 7: 7600-7608. https://doi.org/10.3390/molecules18077600