Analytical Study for the Charge-Transfer Complexes of Rosuvastatin Calcium with π-Acceptors
Abstract
:1. Introduction
2. Results and Discussion
2.1. Spectral Characteristics of the Reaction
2.2. Optimization of Reaction Conditions
2.3. Molar Ratio of the Reaction, Molecular Modeling, and Proposing the Site of Interaction
Acceptor a | Condition | Molar absorptivity (ε × 10−4) | |||
---|---|---|---|---|---|
Reagent conc. (mg mL−1) | Solvent | Time (min) | λmax (nm) | ||
pCA | 4 | Acetonitrile | At once b | 518 | 1.4 |
DDQ (1.9) | 2 | Methanol | At once b | 460 | 3.0 |
TCNE (2.2) | 2 | Acetonitrile | 15 | 412 | 1.8 |
TNB (0.7) | 4 | Acetonitrile | 30 | 435 | 0.64 |
TCNQ (1.7) | 1 | Acetonitrile | 15 | 840 | 4.0 |
Bromanil (1.37) | 5 | Acetonitrile | 5 | 498 | 0.88 |
Chloranil (1.37) | 5 | Acetonitrile | 5 | 460 | 2.2 |
TNF (1.1) | 5 | Acetonitrile | 60 | 412 | 0.25 |
2.4. Development and Validation of the Analytical Methods
2.4.1. Calibration Curves, Linearity and Sensitivity
2.4.2. Precision
2.4.3. Specificity and Interference
Acceptor | Range (μg mL−1) | Intercept | Slope | Correlation coefficient | LOD (μg mL−1) | LOQ (μg mL−1) |
---|---|---|---|---|---|---|
TCNQ | 5–50 | 0.0032 | 0.0388 | 0.9995 | 0.41 | 1.37 |
TNB | 4–30 | 0.0171 | 0.0137 | 0.9992 | 1.52 | 5.07 |
DDQ | 2–40 | 0.0146 | 0.0304 | 0.9990 | 1.14 | 3.80 |
pCA | 10–150 | 0.0211 | 0.0061 | 0.9987 | 1.82 | 6.07 |
TCNE | 5–60 | 0.0062 | 0.0183 | 0.9989 | 4.32 | 14.39 |
Bromanil | 25–100 | 0.0074 | 0.0087 | 0.9993 | 5.11 | 17.02 |
Chloranil | 40–200 | 0.0171 | 0.0021 | 0.9984 | 12.24 | 40.76 |
Acceptor-based method | ROS-Ca (μg mL−1) | Within-assay, n = 6 | Between-assays, n = 6 | ||
---|---|---|---|---|---|
Mean (μg mL −1 ± SD) | RSD | Mean (μg mL −1 ± SD) | RSD | ||
pCA | 50 | 48.78 ± 0.41 | 0.84 | 50.05 ± 0.84 | 1.68 |
DDQ | 20 | 20.51 ± 0.25 | 1.22 | 19.56 ± 0.36 | 1.84 |
TCNE | 40 | 40.57 ± 0.75 | 1.85 | 38.96 ± 0.75 | 1.93 |
TNB | 20 | 20.73 ± 0.23 | 1.11 | 19.55 ± 0.35 | 1.79 |
TCNQ | 40 | 38.78 ± 0.16 | 0.41 | 38.04 ± 0.29 | 0.76 |
Bromanil | 80 | 81.57 ± 0.84 | 1.03 | 78.24 ± 0.81 | 1.04 |
Chloranil | 200 | 198.78 ± 2.69 | 1.35 | 201.05 ± 2.08 | 1.03 |
2.4.4. Ruggedness and Robustness
2.5. Application of the Method to the Analysis of Tablets
Method | Label claim (% ± SD) a | t-values b | F-values b |
---|---|---|---|
pCA | 99.58 ± 1.77 | 1.55 | 2.41 |
DDQ | 100.21 ± 1.43 | 1.26 | 1.57 |
TCNE | 99.54 ± 1.58 | 1.86 | 1.92 |
TNB | 99.82 ± 0.78 | 0.83 | 0.47 |
TCNQ | 100.09 ± 1.96 | 0.61 | 2.20 |
Bromanil | 100.29 ± 1.32 | 1.72 | 1.34 |
Chloranil | 100.46 ± 1.82 | 2.10 | 2.55 |
Reported c | 99.95 ± 1.14 |
3. Experimental Section
3.1. Apparatus
3.2. Chemicals and Reagents
3.3. Preparation of Standard and Tablets Sample Solutions
3.3.1. Preparation of Stock Standard ROS-Ca Solution
3.3.2. Preparation of Tablets Sample Solution
3.4. General Analytical Procedure
3.5. Determination of Molar Ratio
3.6. Molecular Modeling for the CT Complex of ROS-Ca with DDQ
4. Conclusions
Acknowledgments
Conflicts of Interest
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Alzoman, N.Z.; Sultan, M.A.; Maher, H.M.; Alshehri, M.M.; Wani, T.A.; Darwish, I.A. Analytical Study for the Charge-Transfer Complexes of Rosuvastatin Calcium with π-Acceptors. Molecules 2013, 18, 7711-7725. https://doi.org/10.3390/molecules18077711
Alzoman NZ, Sultan MA, Maher HM, Alshehri MM, Wani TA, Darwish IA. Analytical Study for the Charge-Transfer Complexes of Rosuvastatin Calcium with π-Acceptors. Molecules. 2013; 18(7):7711-7725. https://doi.org/10.3390/molecules18077711
Chicago/Turabian StyleAlzoman, Nourah Z., Maha A. Sultan, Hadir M. Maher, Mona M. Alshehri, Tanveer A. Wani, and Ibrahim A. Darwish. 2013. "Analytical Study for the Charge-Transfer Complexes of Rosuvastatin Calcium with π-Acceptors" Molecules 18, no. 7: 7711-7725. https://doi.org/10.3390/molecules18077711