Luteolin Inhibits Inflammatory Responses via p38/MK2/TTP-mediated mRNA Stability
Abstract
:1. Introduction
2. Results and Discussion
2.1. Lut Inhibits the Production of TNF-α and IL-6
2.2. Lut Promotes the Degradation of TNF-α and IL-6 mRNAs
2.3. Lut Affects the mRNAs Stability of TNF-α and IL-6 via the p38/MK2/TTP Signaling Cascade
3. Experimental
3.1. Materials
3.2. BMM Cultures
3.3. MTT Assay
3.4. ELISA
3.5. RNA Isolation and Reverse Transcription
3.6. Real-Time PCR
3.7. Western Blot
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Wu, W.; Li, D.; Zong, Y.; Zhu, H.; Pan, D.; Xu, T.; Wang, T.; Wang, T. Luteolin Inhibits Inflammatory Responses via p38/MK2/TTP-mediated mRNA Stability. Molecules 2013, 18, 8083-8094. https://doi.org/10.3390/molecules18078083
Wu W, Li D, Zong Y, Zhu H, Pan D, Xu T, Wang T, Wang T. Luteolin Inhibits Inflammatory Responses via p38/MK2/TTP-mediated mRNA Stability. Molecules. 2013; 18(7):8083-8094. https://doi.org/10.3390/molecules18078083
Chicago/Turabian StyleWu, Wanling, Dongye Li, Yu Zong, Hong Zhu, Defeng Pan, Tongda Xu, Tao Wang, and Tingting Wang. 2013. "Luteolin Inhibits Inflammatory Responses via p38/MK2/TTP-mediated mRNA Stability" Molecules 18, no. 7: 8083-8094. https://doi.org/10.3390/molecules18078083