Apoptosis-Inducing Effect of Ginsenoside Rg6 on Human Lymphocytoma JK Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Influence of Different Concentrations of GRg6 on Cell Proliferation
Group (μM) | GRg6 | ||
---|---|---|---|
OD ± SD | IR (%) | IC50 | |
Negative | 1.181 ± 0.013 | — | 83.08 μM |
Positive | 0.150 ± 0.003 | 87.27 | |
1.02 | 1.171 ± 0.015 | 0.82 | |
2.04 | 1.147 ± 0.018 | 2.85 | |
4.08 | 1.125 ± 0.011 | 4.74 | |
8.15 | 1.102 ± 0.021 | 6.63 | |
16.31 | 1.094 ± 0.017 | 7.34 | |
32.62 | 1.040 ± 0.014 | 11.94 | |
65.23 | 0.959 ± 0.006 | 18.75 | |
130.46 | 0.169 ± 0.002 | 85.71 | |
260.93 | 0.152 ± 0.002 | 87.10 |
2.2. Hochest33258 Fluorescence Staining to Detect Cell Apoptosis
2.3. Annexin-V FITC/PI Double-staining Method to Detect Cell Apoptosis
2.4. PI Single-Staining Method to Detect the Cell Cycle
2.5. JC-1 Staining Method to Detect Mitochondrial Membrane Potential
2.6. Western Blot to Detect Protein Expression
2.7. Discussion
3. Experimental
3.1. Materials
3.1.1. Cell Strain
3.1.2. Medicine and Groups
3.1.3. Main Reagents and Consumables
3.2. Methods
3.2.1. Cell Culture
3.2.2. CCK-8 Method to Measure Cell Proliferation
3.2.3. Hochest33258 Fluorescence Staining
3.2.4. Annexin-V FITC/PI Double Staining Method to Detect Cell Apoptosis
3.2.5. PI Single-Staining Method to Detect the Cell Cycle
3.2.6. JC-1 Staining Method to Detect Mitochondrial Membrane Potential
3.2.7. Western Blot Method to Detect Protein Expression
3.3. Statistical Analyses
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Chen, B.; Jia, X.-B. Apoptosis-Inducing Effect of Ginsenoside Rg6 on Human Lymphocytoma JK Cells. Molecules 2013, 18, 8109-8119. https://doi.org/10.3390/molecules18078109
Chen B, Jia X-B. Apoptosis-Inducing Effect of Ginsenoside Rg6 on Human Lymphocytoma JK Cells. Molecules. 2013; 18(7):8109-8119. https://doi.org/10.3390/molecules18078109
Chicago/Turabian StyleChen, Bin, and Xiao-Bin Jia. 2013. "Apoptosis-Inducing Effect of Ginsenoside Rg6 on Human Lymphocytoma JK Cells" Molecules 18, no. 7: 8109-8119. https://doi.org/10.3390/molecules18078109