Development of 65 Novel Polymorphic cDNA-SSR Markers in Common Vetch (Vicia sativa subsp. sativa) Using Next Generation Sequencing
Abstract
:1. Introduction
2. Results and Discussion
Marker | Primer sequence (5'-3') | Motif | GenBank Acc. No. | Ta (°C) | BLAST top hit Acc. No. | Description | E-value |
---|---|---|---|---|---|---|---|
GBSSR-VSspS-020 | F: CATTTGGCTGATCCTGTCA R: GGCTTCATCATGAGACAAGAA | (TAT)5 | KF008486 | 55 | L19651.1 | Pisum sativum chloroplast photosystem I 24 kDa light harvesting protein (lhca3) mRNA, complete cds | 7e-92 |
GBSSR-VSspS-023 | F: CCTGCATTCACAACCATTT R: CGCCATCGATGTTTTGTT | (CAT)5 | KF008487 | 55 | None | None | None |
GBSSR-VSspS-024 | F: ACGGTGTTAACGGTCACG R: TCTCCAAACCGACACCAG | (AGA)5 | KF008488 | 55 | AF262939.1 | Pisum sativum chloroplast protein import component Toc159mRNA. complete cds | 1e-160 |
GBSSR-VSspS-028 | F: TGAGCCGTTGACACAACA R: GGCGATCCTCCTACTTGAA | (TGC)5 | KF008489 | 55 | None | None | None |
GBSSR-VSspS-037 | F: GAAACAAGCTGAAGGCCC R: TCAGGAAATGACCAAACCA | (GAA)5 | KF008490 | 55 | X76774.1 | P.sativum mRNA for HMG1 protein | 2e-123 |
GBSSR-VSspS-038 | F: CTCCCCAACTTGTTCCCT R: GGGAACTTGTCGATGTGG | (TTC)5 | KF008491 | 55 | BT143793 | Medicago truncatula clone JCVI-FLMt-3P13 unknown mRNA | 3e-41 |
GBSSR-VSspS-042 | F: GGTTCGAGAGCTTTGCTG R: CTGTGCCACTTGACCTCC | (CGT)5 | KF008492 | 55 | JF965421.1 | Medicago sativa ethylene response factor 11 (ERF11) mRNA, complete cds | 2e-37 |
GBSSR-VSspS-057 | F: GAGGTTTCCGGTGAGGAG R: GTTCCAGCAGGTGAAGCA | (GGT)7 | KF008493 | 55 | XM_003548374.1 | PREDICTED: Glycine max DEAD-box ATP-dependent RNA helicase 31-like (LOC100799999), mRNA | 7e-28 |
GBSSR-VSspS-066 | F: AGGAGAGGCAAGGACCAG R: CACGGCTATTTTCTTCTTTTTC | (GA)10 | KF008494 | 55 | AB078603.1 | Pisum sativum SN4TDR mRNA for 110kDa 4SNc-Tudor domin protein, complet cds | 1e-93 |
GBSSR-VSspS-067 | F: CAAACTTGTCACCACATATACAA R: GGTGGTCACTAGTGGAGGTG | (CCA)5 | KF008495 | 55 | KC218790.1 | Vicia faba clone NAC-VF- 218 microsatellite sequence | 3e-39 |
GBSSR-VSspS-071 | F: GTATTCCTCTGGTGGTGGG R: CACCACCAAGACCTCCAA | (TGG)5 | KF008496 | 55 | XM 003617084.1 | Medicago truncatula Heterogeneous nuclear ribonucleoprotein DO (MTR 5g088220) mRNA, complete cds | 9e-108 |
GBSSR-VSspS-073 | F: CCTCCCAATCCTCCATTC R: CCCTAGTCCTCCAATTTCG | (GTT)5 | KF008497 | 55 | XM_004506417.1 | PREDICTED: Cicer arietinum scarecrow-like protein 6-like (LOC101497219), mRNA | 2e-58 |
GBSSR-VSspS-075 | F: TTCAGCAAGCCCATCATT R: CGTCCGTCCAATCAACAA | (TTA)6 | KF008498 | 55 | JF768700.1 | Lens culinaris microsatellite LcSSR535 sequence | 1e-71 |
GBSSR-VSspS-076 | F: CCTGGTCCCAGAAATGGT R: AAGCCAGAGGGCATTGAT | (CTG)6 | KF008499 | 55 | XM_003602555.1 | Medicago truncatula NAC domin protein (MTR_3g096140) mRNA complete cds | 1e-136 |
GBSSR-VSspS-079 | F: AAAGCAAATTGTTAAAGAAAGGG R: GAGGATGCTGCACATATGTAGTT | (AAT)5 | KF008500 | 55 | None | None | None |
GBSSR-VSspS-080 | F: AATGCATGGATCGAGGTG R: GAATCCATCGGCAACGTA | (TGG)5 | KF008501 | 55 | XM_004510745.1 | PREDICTED: Cicer arietinum uncharacterized LOC 101512367 (LOC 101512367), transcript variant X3, mRNA | 6e-110 |
GBSSR-VSspS-088 | F: CGAAGAGGTAAATGACGCC R: AGTGACCTATATTTAGCATCGTT | (TGG)5 | KF008502 | 55 | None | None | None |
GBSSR-VSspS-090 | F: AGACGCACCACAACAGAAA R: GGGCTAGACATGGCACAA | (AGC)6 | KF008503 | 55 | BT149661.1 | Medicago truncatula clone JCVI-FLMt-1507 unknown mRNA | 7e-62 |
GBSSR-VSspS-091 | F: CCAAACCAGCAAGAGCAG R: GAGCAGCGTTGTCTCGTC | (CTT)5 | KF008504 | 55 | XM_003594377.1 | Medicago trancatula Endoglucanase (MTR_2g028480) mRNA complete cds | e-122 |
GBSSR-VSspS-099 | F: ATCCATGCCTCTTTTGCC R: AGCCTCATTTCAGCAGCA | (TCT)5 | KF008505 | 55 | BT137674.1 | Medicago truncatula clone JCVIMt-1708 unknown mRNA | 3e-77 |
GBSSR-VSspS-102 | F: TTCAACGGAGATGGATCG R: CGTCTTCTTTCAGAGGCG | (GTT)5 | KF008506 | 55 | X59773.1 | Pisum sativum mRNA for P protein. a part of glycine cleavage complex | 0.0 |
GBSSR-VSspS-107 | F: TGGTTTCTTTCTAAAGGGGTG R: CGGCTCGATGGACAGTAG | (GTT)5 | KF008507 | 55 | BT146949.1 | Medicago truncatula clone JCVI-FLMt-19I1 unknown | e-127 |
GBSSR-VSspS-115 | F: CATAAACAAGGGCAAGAAAA R: GAGGAAAACATTGGTGGGA | (TGC)6 | KF008508 | 55 | XM_004501895.1 | PREDICTED: Cicer arietinum nucleobase- ascorbate transporter 6-like (LOC101504609), transcript X2, mRNA | 6e-59 |
GBSSR-VSspS-117 | F: CGGTGCACTAAGTGGGAA R: TTAATGATGGTGGCGAGG | (AGG)5 | KF008509 | 55 | BT135350.1 | Medicago truncatula clone JCVI-FLMt-9H4 unknown mRNA | 2e-114 |
GBSSR-VSspS-118 | F: GCATTTCCCTTGGTCTCC R: CAGAAAGAGCAACCGTGC | (TGG)5 | KF008510 | 55 | XM_004514512.1 | PREDICTED: Cicer arietinum N-alpha-acetyltransferase 10-like (LOC101504041), mRNA | 3e-68 |
GBSSR-VSspS-119 | F: CACCACCAAGACCTCCAA R: CCATCATCATCACCAGCC | (ACC)5 | KF008511 | 55 | XM_003617084.1 | Medicago truncatula Heterogeneous nuclear ribonucleoprotein D0 (MTR_5g088220), complete cds | 9e-98 |
GBSSR-VSspS-125 | F: GGCCGGTATTCGTCAACT R: CCCCGTATTTTCTCGGTC | (TGG)5 | KF008512 | 55 | XM_004510745.1 | PREDICTED: Cicer arietinum uncharacterized LOC 101512367 (LOC101512367), transcript variant X3, mRNA | 2e-134 |
GBSSR-VSspS-126 | F: TGGCGCTTATCGCTATGT R: TCCACTCATTCCACTCGT | (TG)7 | KF008513 | 55 | XM_003623363.1 | Medicago truncatula Patellin-6 (TR_7G070480) mRNA, complete cds | 5e-81 |
GBSSR-VSspS-129 | F: AGGAGAGGCAAGGACCAG R: CTTTTTCTCTAACTCATTCATGTC | (GA)10 | KF008514 | 55 | AB0786031 | Pisum sativum SN4TDR mRNA for 110kDa 4SNc-Tudor domain protein, complete cds | 8e-117 |
GBSSR-VSspS-135 | F: TGGTGGAGATTTGTTGGG R: CTTCATCTTCCCACACCG | (TGG)5 | KF008515 | 55 | BT136030.1 | Medicago truncatula clone JCVI-FLMt-16L14unknown mRNA | 4e-40 |
GBSSR-VSspS-138 | F: CGGAGTTCACATAAAACATACTAC R: TGGGAGTGTTGAGATGGG | (TTA)7 | KF008516 | 55 | AB176563.1 | Vicia faba MET mRNA for type 2 metallothionein, complete cds | 6e-98 |
GBSSR-VSspS-140 | F: TTGCTTTGATGTTTGGAGC R: CCCTAAATTCCCAACCCA | (GGT)7 | KF008517 | 55 | XM_003613856.1 | Medicago truncatula Cysteine-rich receptor-like protein kinase (MTR_5g042440) mRNA, complete cds | 3e-86 |
GBSSR-VSspS-156 | F: GGCCAATTTAGCGAGCTT R: CACTATCATCAACCTCTAACGGA | (GTG)5 | KF008519 | 55 | BT134176.1 | Medicago truncatula clone JCVI-FLMt-15D24 unknown mRNA | 2e-118 |
GBSSR-VSspS-158 | F: TGAGCTTATTGCCAGTGGA R: CCATGTCATCATCGGATTC | (TGG)5 | KF008520 | 55 | KC218603.1 | Vicia faba clone NAC-VF-31 microsatellite sequence | 7e-124 |
GBSSR-VSspS-162 | F: GAGACAGTGGAAGTATCGGC R: CACAGCAAATGCATCGGT | (AAG)6 | KF008521 | 55 | BT146412.1 | Medicago truncatula clone JCVI-FLMt-21014 unknown mRNA | 1e-75 |
GBSSR-VSspS-166 | F: GTGGCCATGATCCATTTG R: TTCCTCGAGAGGGAAAGC | (TGG)5 | KF008522 | 55 | XM_003605932.1 | Medicago truncatula DnaJ (MTR_4g50420) mRNA complete cds | 0.0 |
GBSSR-VSspS-172 | F: GCTTTGGAAGAGCCCAAT R: TCCAGGATTGTAACCCCC | (TGG)5 | KF008523 | 55 | XM_003617084.1 | Medicago truncatula Heterogeneous nuclear ribonucleoprotein D0 (MTR_5g088220) mRNA, complete cds | 5e-90 |
GBSSR-VSspS-173 | F: GGGCACGGTGGTCACTA R: TGACTACCACCACCTCCG | (TGG)5 | KF008524 | 55 | AJ831469.1 | Pisum sativum mRNA for putative glycine rich protein precursor (grp1 gene) | 2e-13 |
GBSSR-VSspS-179 | F: AGCTATGCGAGAGGCTCC R: CTGTGGGAAGGCACATCT | (TGA)6 | KF008525 | 55 | None | None | None |
GBSSR-VSspS-181 | F: CACTGTGACTCAGTTTCGTTG R: CGATTTTGAACCCTAACCG | (TTC)5 | KF008526 | 55 | None | None | None |
GBSSR-VSspS-182 | F: GCGTTGTGGCGTATTTCT R: TGGAGGAAAGGAAACTACTCA | (GCA)6 | KF008527 | 55 | AB676029.1 | Lathyrus japonicas DNA, 61 locus, haplotype: D | 4e-62 |
GBSSR-VSspS-185 | F: CTCCTCAATTTTCCCCCA R: TTTGGTGCGATTGTTTCC | (CAT)5 | KF008528 | 55 | Ap009676.1 | Lotus japonicas genomic DNA, clone: LjT30I08, TM0492 | 4e-26 |
GBSSR-VSspS-187 | F: CCAGGTTGCTTTCCTTACTTT R: TTAGCCCTCAAAGCCTCC | (ATC)5 | KF008529 | 55 | X54359.1 | P.sativum mRNA of cDNA clone 26g | 2e-154 |
GBSSR-VSspS-192 | F: AGGGTCTTCCTTCCCACA R: TATGGTGACACGTTCGCA | (ATC)5 | KF008530 | 55 | XM_004505980.1 | PREDICTED: Cicer arietinum uncharacterized LOC101500025 (LOC101500025), transcript variant X2, mRNA | 1e-100 |
GBSSR-VSspS-203 | F: TCCATCTGGTTGGTGGTG R: GAAAGCCAATTTTTCAGCAA | (GTT)7 | KF008531 | 55 | BT147294.1 | Medicago truncatula clone JCVI-FLMt-15A20 unknown mRNA | 2e-50 |
GBSSR-VSspS-217 | F: CCATCGCCACCACCA R: TCCCGGAACAAAAATCAA | (AAC)7 | KF008532 | 55 | EF447278.1 | Pisum sativum cultivar Finale SYM8 (SYM8) gene, partial cds | 5e-58 |
GBSSR-VSspS-245 | F: CAATAGGGGGACCCTTCA R: GCTGCAAGCTGCTACCAT | (GGA)5 | KF008533 | 55 | HQ439603.1 | Phalaenopsis hybrid cultivar candidate developmental transcription factor TCP1 mRNA, partial cds | 5e-04 |
GBSSR-VSspS-247 | F: GGTTCAATACGATCCATAGAATA R: TGATCGCCAATTCTGGAC | (CAC)6 | KF008534 | 55 | KC294548.1 | Aeschynomene ciliate voucher IRRI 13078 cyclophilin 1 (CYP1) gene, complete cds | 3e-46 |
GBSSR-VSspS-249 | F: AAAACATGGTTGAGTGTTTTTG R: TAACCCTCTCGGTTTCGG | (ATA)5 | KF008535 | 55 | KC218749.1 | Vicia faba clone NAC-VF-177 microsatellite sequence | 2e-55 |
GBSSR-VSspS-251 | F: TGGTGGACGTCACTATGGA R: CATGGTGCTTCCGACAAT | (TGG)5 | KF008536 * | 55 | KC218790.1 | Vicia faba clone NAC-VF-218 microsatellite sequence | 5e-30 |
GBSSR-VSspS-252 | F: CATGGTGCTTCCGACAAT R: TCGAAATCAGGACTTACCACA | (CCA)5 | KF008536 * | 55 | KC218790.1 | Vicia faba clone NAC-VF-218 microsatellite sequence | 5e-30 |
GBSSR-VSspS-262 | F: ATTGGGCCCTCTTTTTGA R: GGGGGTAGAAAAGTTGCG | (AT)7 | KF008537 | 55 | XM_004500781.1 | PREDICTED: Cicer arietinum dolichyl- diphoshooligosaccharide-protein glycosyltransferase subunit 1A-like (LOC101502563), mRNA | 1e-37 |
GBSSR-VSspS-268 | F: AAATTTGTCTGACGAAAAACG R: TGCTTGAGAGTGCCATCA | (TAC)5 | KF008538 | 55 | BT144509.1 | Medicago truncatula clone JCVI-FLMt-13M6 unknown mRNA | 2e-47 |
GBSSR-VSspS-269 | F: TTCCATTTATCCTCCTATCCTCT R: CTTGAATGCGAAACGAGG | (CGC)5 | KF008539 | 55 | XM_004507695.1 | PREDICTED: Cicer arietinum eukaryotic translation initiation factorisoform 4G-1-like (LOC101492356), mRNA | 1e-56 |
GBSSR-VSspS-284 | F: TGGAAGGAAATGGCAGTG R: ATCCGTTTCGGATTGGTT | (GCA)5 | KF008540 | 55 | JX539287.1 | Vigna radiata cultivar MCV-1 clone GGSSR_911 microsatellit sequence | 2e-120 |
GBSSR-VSspS-291 | F: CCCAACCGAACCACTTATT R: TAATAGCTCCGGCCCAGT | (CTA)5 | KF008541 | 55 | None | None | None |
GBSSR-VSspS-301 | F: AACCAAACAACAATGGGTT R: TCAACCGGTGAAAGATGG | (CAA)5 | KF008542 | 55 | JN849865.1 | Medicago falcata voucher PI494662A caffeic acid-O- methyltransferase (COMT) gene.exon 1 and partial cds | 2e-128 |
GBSSR-VSspS-304 | F: CCGTTCTACGCAATTCTCC R: CGACCAAGAACACCAGGA | (TTC)5 | KF008543 | 55 | XM_003593951.1 | Medicago truncatula hypothetical protein (MTR_2g020190) mRNA,complete cds | 1e-55 |
GBSSR-VSspS-305 | F: CATGAAAGAGTTTTGCACCTT R: CCGACGACGAGATTGAGA | (GCA)5 | KF008544 | 55 | None | None | None |
GBSSR-VSspS-308 | F: TGAGAGCATAGACAGCAAACA R: TGGATTTGGTCGCATAGC | (AAC)5 | KF008545 | 55 | None | None | None |
GBSSR-VSspS-309 | F: TCTTCAAAAGAGTACAAAAGGGA R: GAATTGGACACCTTGGCA | (AAT)5 | KF008546 | 55 | BT137399.1 | Medicago truncatula clone JCVI-FlMt-19L22 unknown mRNA | 5e-124 |
GBSSR-VSspS-310 | F: GGGTGCCCTAGCATTTGT R: ATCTCCGGCGTCAGTTTC | (CTC)6 | KF008547 | 55 | M69105.1 | Pisum sativum outer membrane protein (OM14) mRNA, complete cds | 9e-96 |
GBSSR-VSspS-311 | F: TTGAGGCGGTGTTGGTAG R: ATGTCATGGCCAACTGCT | (GGA)6 | KF008548 | 55 | None | None | None |
GBSSR-VSspS-313 | F: GAACAATGCAGCCTGGAA R: GCTGCAATCGCATTCTCT | (TTG)5 | KF008549 | 55 | XM_003613196.1 | Medicago truncatula U-box domin containing protein (MTR_5g034440) mRNA, complete cds | 2e-112 |
Marker | NA | MAF | HO | HE | PIC |
---|---|---|---|---|---|
GBSSR-VSspS-020 | 4 | 0.63 | 0.00 | 0.51 | 0.43 |
GBSSR-VSspS-023 | 7 | 0.34 | 0.00 | 0.75 | 0.71 |
GBSSR-VSspS-024 | 7 | 0.47 | 0.00 | 0.71 | 0.67 |
GBSSR-VSspS-028 | 4 | 0.38 | 0.00 | 0.73 | 0.68 |
GBSSR-VSspS-037 | 4 | 0.38 | 0.00 | 0.71 | 0.65 |
GBSSR-VSspS-038 | 7 | 0.28 | 0.00 | 0.79 | 0.76 |
GBSSR-VSspS-042 | 2 | 0.84 | 0.00 | 0.26 | 0.23 |
GBSSR-VSspS-057 | 8 | 0.36 | 0.03 | 0.77 | 0.74 |
GBSSR-VSspS-066 | 7 | 0.25 | 0.00 | 0.83 | 0.81 |
GBSSR-VSspS-067 | 6 | 0.50 | 0.00 | 0.67 | 0.62 |
GBSSR-VSspS-071 | 3 | 0.50 | 0.00 | 0.62 | 0.54 |
GBSSR-VSspS-073 | 8 | 0.31 | 0.00 | 0.81 | 0.79 |
GBSSR-VSspS-075 | 6 | 0.38 | 0.03 | 0.76 | 0.72 |
GBSSR-VSspS-076 | 3 | 0.56 | 0.00 | 0.57 | 0.50 |
GBSSR-VSspS-079 | 6 | 0.55 | 0.00 | 0.64 | 0.60 |
GBSSR-VSspS-080 | 3 | 0.50 | 0.00 | 0.59 | 0.51 |
GBSSR-VSspS-088 | 5 | 0.38 | 0.00 | 0.74 | 0.70 |
GBSSR-VSspS-090 | 7 | 0.41 | 0.00 | 0.75 | 0.71 |
GBSSR-VSspS-091 | 4 | 0.47 | 0.00 | 0.68 | 0.64 |
GBSSR-VSspS-099 | 4 | 0.56 | 0.00 | 0.60 | 0.54 |
GBSSR-VSspS-102 | 8 | 0.44 | 0.03 | 0.75 | 0.73 |
GBSSR-VSspS-107 | 3 | 0.66 | 0.00 | 0.51 | 0.45 |
GBSSR-VSspS-115 | 4 | 0.48 | 0.03 | 0.54 | 0.44 |
GBSSR-VSspS-117 | 6 | 0.41 | 0.00 | 0.71 | 0.67 |
GBSSR-VSspS-118 | 3 | 0.53 | 0.00 | 0.61 | 0.54 |
GBSSR-VSspS-119 | 2 | 0.65 | 0.00 | 0.46 | 0.35 |
GBSSR-VSspS-125 | 3 | 0.41 | 0.00 | 0.63 | 0.56 |
GBSSR-VSspS-126 | 7 | 0.25 | 0.00 | 0.82 | 0.79 |
GBSSR-VSspS-129 | 7 | 0.25 | 0.00 | 0.81 | 0.79 |
GBSSR-VSspS-135 | 5 | 0.50 | 0.00 | 0.62 | 0.55 |
GBSSR-VSspS-138 | 11 | 0.32 | 0.06 | 0.83 | 0.81 |
GBSSR-VSspS-140 | 8 | 0.22 | 0.03 | 0.81 | 0.79 |
GBSSR-VSspS-151 | 5 | 0.67 | 0.03 | 0.52 | 0.48 |
GBSSR-VSspS-156 | 5 | 0.66 | 0.00 | 0.53 | 0.50 |
GBSSR-VSspS-158 | 5 | 0.59 | 0.00 | 0.57 | 0.52 |
GBSSR-VSspS-162 | 6 | 0.53 | 0.00 | 0.66 | 0.63 |
GBSSR-VSspS-166 | 6 | 0.38 | 0.00 | 0.74 | 0.70 |
GBSSR-VSspS-172 | 3 | 0.41 | 0.00 | 0.65 | 0.57 |
GBSSR-VSspS-173 | 4 | 0.58 | 0.00 | 0.58 | 0.53 |
GBSSR-VSspS-179 | 8 | 0.56 | 0.03 | 0.64 | 0.61 |
GBSSR-VSspS-181 | 11 | 0.28 | 0.00 | 0.85 | 0.84 |
GBSSR-VSspS-182 | 7 | 0.38 | 0.00 | 0.76 | 0.73 |
GBSSR-VSspS-185 | 10 | 0.31 | 0.03 | 0.82 | 0.80 |
GBSSR-VSspS-187 | 5 | 0.44 | 0.00 | 0.69 | 0.64 |
GBSSR-VSspS-192 | 3 | 0.63 | 0.00 | 0.51 | 0.43 |
GBSSR-VSspS-203 | 6 | 0.34 | 0.00 | 0.76 | 0.72 |
GBSSR-VSspS-217 | 11 | 0.28 | 0.00 | 0.83 | 0.81 |
GBSSR-VSspS-245 | 7 | 0.28 | 0.00 | 0.81 | 0.78 |
GBSSR-VSspS-247 | 6 | 0.69 | 0.03 | 0.49 | 0.46 |
GBSSR-VSspS-249 | 6 | 0.56 | 0.00 | 0.64 | 0.61 |
GBSSR-VSspS-251 | 5 | 0.45 | 0.00 | 0.70 | 0.66 |
GBSSR-VSspS-252 | 3 | 0.50 | 0.00 | 0.61 | 0.53 |
GBSSR-VSspS-262 | 9 | 0.47 | 0.00 | 0.72 | 0.70 |
GBSSR-VSspS-268 | 3 | 0.47 | 0.00 | 0.64 | 0.57 |
GBSSR-VSspS-269 | 4 | 0.50 | 0.00 | 0.58 | 0.49 |
GBSSR-VSspS-284 | 6 | 0.44 | 0.00 | 0.66 | 0.60 |
GBSSR-VSspS-291 | 5 | 0.53 | 0.00 | 0.64 | 0.59 |
GBSSR-VSspS-301 | 3 | 0.53 | 0.00 | 0.58 | 0.50 |
GBSSR-VSspS-304 | 7 | 0.46 | 0.00 | 0.68 | 0.63 |
GBSSR-VSspS-305 | 3 | 0.69 | 0.00 | 0.48 | 0.43 |
GBSSR-VSspS-308 | 6 | 0.54 | 0.00 | 0.66 | 0.63 |
GBSSR-VSspS-309 | 8 | 0.34 | 0.00 | 0.78 | 0.75 |
GBSSR-VSspS-310 | 8 | 0.38 | 0.03 | 0.75 | 0.71 |
GBSSR-VSspS-311 | 7 | 0.44 | 0.00 | 0.73 | 0.70 |
GBSSR-VSspS-313 | 5 | 0.44 | 0.00 | 0.71 | 0.67 |
Mean | 5.7 | 0.460 | 0.006 | 0.670 | 0.624 |
3. Experimental
3.1. Plant Material
No. | Temp. ID | USDA-ARS No. | Country of origin | Geographical region of origin |
---|---|---|---|---|
1 | K193581 | PI 226487 | Iran | West Asia |
2 | K193582 | PI 284058 | Czech Republic | Central Europe |
3 | K193583 | PI 284068 | Germany | Western Central Europe |
4 | K193584 | PI 284078 | Greece | South East Europe |
5 | K193585 | PI 284402 | Cyprus | Europe |
6 | K193586 | PI 284409 | Cyprus | Europe |
7 | K193587 | PI 284470 | Czech Republic | Central Europe |
8 | K193588 | PI 284471 | Hungary | Central Europe |
9 | K193589 | PI 308111 | Slovakia | Central Europe |
10 | K193590 | PI 308118 | Slovakia | Central Europe |
11 | K193591 | PI 325513 | Soviet Union | Eurasian |
12 | K193592 | PI 381065 | Iran | West Asia |
13 | K193593 | PI 393870 | Poland | Central Europe |
14 | K193594 | PI 393871 | Romania | Central and South East Europe |
15 | K193595 | PI 393872 | Czech Republic | Central Europe |
16 | K193596 | PI 393873 | Czech Republic | Central Europe |
17 | K193597 | PI 393877 | Netherlands | North West Europe |
18 | K193598 | PI 393878 | France | West Europe |
19 | K193599 | PI 393891 | Bulgaria | South East Europe |
20 | K193600 | PI 393904 | Belgium | West Europe |
21 | K193601 | PI 393907 | Finland | North Europe |
22 | K193602 | PI 393909 | Israel | Middle East Asia |
23 | K193603 | PI 393910 | United Kingdom | Europe |
24 | K193604 | PI 393912 | Portugal | South West Europe |
25 | K193605 | PI 493307 | Portugal | South West Europe |
26 | K193606 | PI 517191 | Morocco | North Africa |
27 | K193607 | PI 533741 | Spain | South West Europe |
28 | K193608 | PI 533743 | Spain | South West Europe |
29 | K193609 | PI 557498 | Turkey | South East Europe |
30 | K193610 | PI 577751 | China | East Asia |
31 | K193611 | PI 628288 | Uzbekistan | Central Europe |
32 | K193612 | PI 664293 | Tajikistan | Central Europe |
3.2. cDNA Preparation
3.3. Library Preparation
3.4. 454 Pyrosequencing
3.5.Discovery of cDNA-SSR Markers
3.6. Data Analysis
4. Conclusions
Supplementary Materials
Acknowledgments
Conflicts of Interest
References
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Chung, J.-W.; Kim, T.-S.; Suresh, S.; Lee, S.-Y.; Cho, G.-T. Development of 65 Novel Polymorphic cDNA-SSR Markers in Common Vetch (Vicia sativa subsp. sativa) Using Next Generation Sequencing. Molecules 2013, 18, 8376-8392. https://doi.org/10.3390/molecules18078376
Chung J-W, Kim T-S, Suresh S, Lee S-Y, Cho G-T. Development of 65 Novel Polymorphic cDNA-SSR Markers in Common Vetch (Vicia sativa subsp. sativa) Using Next Generation Sequencing. Molecules. 2013; 18(7):8376-8392. https://doi.org/10.3390/molecules18078376
Chicago/Turabian StyleChung, Jong-Wook, Tae-Sung Kim, Sundan Suresh, Sok-Young Lee, and Gyu-Taek Cho. 2013. "Development of 65 Novel Polymorphic cDNA-SSR Markers in Common Vetch (Vicia sativa subsp. sativa) Using Next Generation Sequencing" Molecules 18, no. 7: 8376-8392. https://doi.org/10.3390/molecules18078376