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Article

Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery

by
Eszter Molnár
1,2,†,
Soujanya Kuntam
3,†,
Pradeep Kumar Reddy Cingaram
3,
Begüm Peksel
3,
Bhavyashree Suresh
3,
Gabriella Fábián
2,
Liliána Z. Fehér
4,
Attila Bokros
2,
Ágnes Medgyesi
2,
Ferhan Ayaydin
3 and
László G. Puskás
1,2,4,*
1
Laboratory of Functional Genomics, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, Szeged H-6726, Hungary
2
Avicor Ltd., Alsó kikötő sor 11, Szeged H-6726, Hungary
3
Cellular Imaging Laboratory, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62., Szeged H-6726, Hungary
4
Avidin Ltd., Alsó kikötő sor 11, Szeged H-6726, Hungary
*
Author to whom correspondence should be addressed.
†
The authors contributed equally to this work.
Molecules 2013, 18(8), 9999-10013; https://doi.org/10.3390/molecules18089999
Submission received: 26 June 2013 / Revised: 13 August 2013 / Accepted: 14 August 2013 / Published: 20 August 2013
(This article belongs to the Special Issue Reagents and Methods for Protein Target Identification)
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Abstract

Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. Compounds with high native fluorescence were selected from a 14,585-member library and further tested on living cells under the microscope. Eleven compartment-specific, cell-permeable (or plasma membrane-targeted) fluorochromes were identified. Their cytotoxicity was tested and found that between 1–10 micromolar range, they were non-toxic even during long-term incubations.
Keywords: fluorescent dye discovery; high-throughput screening; small molecule microarray; confocal laser scanning microscopy fluorescent dye discovery; high-throughput screening; small molecule microarray; confocal laser scanning microscopy

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MDPI and ACS Style

Molnár, E.; Kuntam, S.; Cingaram, P.K.R.; Peksel, B.; Suresh, B.; Fábián, G.; Fehér, L.Z.; Bokros, A.; Medgyesi, Á.; Ayaydin, F.; et al. Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery. Molecules 2013, 18, 9999-10013. https://doi.org/10.3390/molecules18089999

AMA Style

Molnár E, Kuntam S, Cingaram PKR, Peksel B, Suresh B, Fábián G, Fehér LZ, Bokros A, Medgyesi Á, Ayaydin F, et al. Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery. Molecules. 2013; 18(8):9999-10013. https://doi.org/10.3390/molecules18089999

Chicago/Turabian Style

Molnár, Eszter, Soujanya Kuntam, Pradeep Kumar Reddy Cingaram, Begüm Peksel, Bhavyashree Suresh, Gabriella Fábián, Liliána Z. Fehér, Attila Bokros, Ágnes Medgyesi, Ferhan Ayaydin, and et al. 2013. "Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery" Molecules 18, no. 8: 9999-10013. https://doi.org/10.3390/molecules18089999

APA Style

Molnár, E., Kuntam, S., Cingaram, P. K. R., Peksel, B., Suresh, B., Fábián, G., Fehér, L. Z., Bokros, A., Medgyesi, Á., Ayaydin, F., & Puskás, L. G. (2013). Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery. Molecules, 18(8), 9999-10013. https://doi.org/10.3390/molecules18089999

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