Protective Effects of Alkaloid Compounds from Nelumbinis Plumula on tert-Butyl Hydroperoxide-Induced Oxidative Stress
Abstract
:1. Introduction
2. Results and Discussion
2.1. Total Alkaloid Content and DPPH Radical-Scavenging Activities of Nelumbinis Plumula Total Alkaloid (NPA)
NPA concentration (μg/mL) | Control | |||||
---|---|---|---|---|---|---|
12.5 | 25 | 50 | 100 | 200 | Ascorbic acid * | |
Percentage of inhibition (%) | 11.95 ± 0.7 a | 16.62 ± 1.5 a | 54.46 ± 4.9 b | 67.19 ± 5.7 c | 76.8 ± 4.6 d | 88.52 ± 7.8 e |
2.2. UV-Vis Spectrophotometric Analysis and HPLC Analysis of NPA
2.3. Protective Effect of NPA and its Alkaloid Compounds on Cytotoxicity in t-BHP-Induced HepG2 Cells
2.4. Effect of Neferine on LDH Leakage of HepG2
2.5. Protective Effect of Neferine on ROS Generation
2.6. Protective Effect of Neferine on Thiobarbituric Acid-Reactive Substance (TBARS)
2.7. Effect of Neferine on Intracellular GSH Production in HepG2
3. Experimental
3.1. Reagents
3.2. Preparation of Total Alkaloids of Nelumbinis Plumula (NPA)
3.3. Determination of Total Alkaloid Content (TAT)
3.4. UV–Vis Spectrophotometric and High-Performance Liquid Chromatography (HPLC) Analysis of Alkaloid Compounds in NPA
3.5. Determination of DPPH Radical-Scavenging Activity
3.6. Cell Culture
3.7. Cell Viability Assay
3.8. Measurement of LDH Leakage
3.9. Induction of Intracellular ROS Production in HepG2 Cells
3.10. Thiobarbituric Acid-Reactive Substance (TBARS) Assay in HepG2 Cells
3.11. Glutathione Estimation
3.12. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Xie, Y.; Zhang, Y.; Zhang, L.-T.; Zeng, S.-X.; Guo, Z.-B.; Zheng, B.-D. Protective Effects of Alkaloid Compounds from Nelumbinis Plumula on tert-Butyl Hydroperoxide-Induced Oxidative Stress. Molecules 2013, 18, 10285-10300. https://doi.org/10.3390/molecules180910285
Xie Y, Zhang Y, Zhang L-T, Zeng S-X, Guo Z-B, Zheng B-D. Protective Effects of Alkaloid Compounds from Nelumbinis Plumula on tert-Butyl Hydroperoxide-Induced Oxidative Stress. Molecules. 2013; 18(9):10285-10300. https://doi.org/10.3390/molecules180910285
Chicago/Turabian StyleXie, Yong, Yi Zhang, Long-Tao Zhang, Shao-Xiao Zeng, Ze-Bin Guo, and Bao-Dong Zheng. 2013. "Protective Effects of Alkaloid Compounds from Nelumbinis Plumula on tert-Butyl Hydroperoxide-Induced Oxidative Stress" Molecules 18, no. 9: 10285-10300. https://doi.org/10.3390/molecules180910285