Effects of Heat Acclimation on Photosynthesis, Antioxidant Enzyme Activities, and Gene Expression in Orchardgrass under Heat Stress
Abstract
:1. Introduction
2. Results
2.1. Physiological Responses to Heat Stress Following Heat Preconditioning and Non-Preconditioning
2.2. Effects of Preconditioning and Non-preconditioning on Antioxidant Enzyme Activity Under Heat Stress
2.3. Assessment of Antioxidant Enzyme Gene Expression under Heat Stress
3. Discussion
4. Experimental Section
4.1. Plant Materials and Treatments
4.2. Photosynthetic Characteristics and Cell Membrane Stability
4.3. Antioxidant Enzymes and Lipid Peroxidation
4.4. Gene Expression
Gene | Species | Accession | Sequence of Primers |
---|---|---|---|
Cu/ZnSOD | Orchardgrass | HO131072.1 | F: 5′-GGCATACTGTCACTCTAAG-3′ |
R: 5′- CAACTCCAGGTCATATCG-3′ | |||
CAT | Orchardgrass | HO179331.1 | F: 5′-GCGACGGCATGAAGTTCCC-3′ |
R: 5′-CGGTTGACGAGCGTGTAGGTG-3′ | |||
POD | Orchardgrass | HO125605.1 | F: 5′-GCCTCCGTGCTGCTCGAC-3′ |
R: 5′-CCAGGATGTCGGAGCAGGAGA-3′ | |||
β-actin | Orchardgrass | HO135499.1 | F: 5′-GTGTTGGATTCTGGTGATGGTGT-3′ |
R: 5′-GGCAGTGGTGGTGAAGGAGTAA-3′ |
4.5. Statistical Analysis
5. Conclusions
Abbreviations
CAT | Catalase |
Ci | Intercellular CO2 concentration |
EL | Electrolyte leakage |
Gs | Stomatal conductance |
HA | Heat acclimated |
MDA | Malondialdehyde |
NA | Non-acclimated |
Pn | Net photosynthetic rate |
POD | Guaiacol peroxidase |
SOD | Superoxide dismutase |
Tr | Transpiration rates |
WUE | Water use efficiency |
Supplementary Materials
Supplementary Files
Supplementary File 1Acknowledgments
Author Contributions
Conflicts of Interest
References
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Zhao, X.X.; Huang, L.K.; Zhang, X.Q.; Li, Z.; Peng, Y. Effects of Heat Acclimation on Photosynthesis, Antioxidant Enzyme Activities, and Gene Expression in Orchardgrass under Heat Stress. Molecules 2014, 19, 13564-13576. https://doi.org/10.3390/molecules190913564
Zhao XX, Huang LK, Zhang XQ, Li Z, Peng Y. Effects of Heat Acclimation on Photosynthesis, Antioxidant Enzyme Activities, and Gene Expression in Orchardgrass under Heat Stress. Molecules. 2014; 19(9):13564-13576. https://doi.org/10.3390/molecules190913564
Chicago/Turabian StyleZhao, Xin Xin, Lin Kai Huang, Xin Quan Zhang, Zhou Li, and Yan Peng. 2014. "Effects of Heat Acclimation on Photosynthesis, Antioxidant Enzyme Activities, and Gene Expression in Orchardgrass under Heat Stress" Molecules 19, no. 9: 13564-13576. https://doi.org/10.3390/molecules190913564