Enhanced Production of Botrallin and TMC-264 with in Situ Macroporous Resin Adsorption in Mycelial Liquid Culture of the Endophytic Fungus Hyalodendriella sp. Ponipodef12
Abstract
:1. Introduction
2. Results and Discussion
2.1. Kinetics of Hyalodendriella sp. Ponipodef12 in Normal Batch Culture
2.2. Effects of the Resins on Mycelial Growth and Production of Botrallin and TMC-264
Resin | Mycelia Biomass (g dw/L) | Botrallin Yield (mg/L) | TMC-264 Yield (mg/L) | Yield of Botrallin Plus TMC-264 (mg/L) |
---|---|---|---|---|
CK | 7.34 ± 0.03 a | 19.52 ± 1.25 cd | 3.77 ± 0.43 g | 23.29 ± 0.82 def |
X-5 | 7.15 ± 0.22 a | 18.61 ± 0.90 d | 5.56 ± 0.17 e | 24.17 ± 0.82 de |
D-101 | 6.73 ± 0.15 c | 16.00 ± 1.83 e | 5.85 ± 0.61 e | 21.85 ± 1.98 f |
HPD-100 | 6.35 ± 0.29 e | 20.14 ± 1.31 c | 4.57 ± 0.84 f | 24.71 ± 2.10 d |
D1300 | 6.91 ± 0.25 b | 21.04 ± 2.08 c | 1.98 ± 0.13 h | 23.02 ± 2.19 def |
D3520 | 6.94 ± 0.45 b | 25.61 ± 0.69 b | 4.44 ± 0.27 f | 30.05 ± 0.95 b |
AB-8 | 6.60 ± 0.40 c | 20.63 ± 0.13 c | 4.45 ± 0.02 f | 25.08 ± 0.13 d |
DM130 | 6.68 ± 0.29 c | 20.15 ± 1.16 c | 7.25 ± 0.23 c | 27.40 ± 0.92 c |
DS-8 | 6.80 ± 0.46 b,c | 14.45 ± 0.20 f | 5.35 ± 0.05 e | 19.79 ± 0.14 g |
DM-301 | 7.07 ± 0.07 b | 27.98 ± 0.01 a | 33.86 ± 0.02 a | 61.84 ± 0.02 a |
ADS-17 | 6.94 ± 0.16 b | 20.30 ± 0.89 c | 11.40 ± 0.46 b | 31.70 ± 0.49 b |
S-8 | 6.64 ± 0.13 cd | 4.62 ± 0.22 g | 5.40 ± 0.15 e | 10.01 ± 0.30 h |
NKA-9 | 6.48 ± 0.37 de | 24.04 ± 0.67 b | 6.31 ± 0.34 d | 30.35 ± 0.91 b |
DA-201 | 7.26 ± 0.17 a | 18.75 ± 0.03 d | 3.82 ± 0.43 g | 22.57 ± 0.40 f |
2.3. Effects of DM-301 Resin on Mycelial Growth and Production of Botrallin and TMC-264
Conc. of DM-301 Resin (%, g/mL) | Mycelia Biomass (g dw/L) | Botrallin Yield (mg/L) | TMC-264 Yield (mg/L) | Yield of Botrallin Plus TMC-264 (mg/L) |
---|---|---|---|---|
0.00 | 7.34 ± 0.08 a | 19.55 ± 0.06 j | 3.68 ± 0.05 k | 23.23 ± 0.07 k |
0.63 | 7.36 ± 0.01 a | 21.02 ± 0.42 i | 4.40 ± 0.12 j | 25.42 ± 0.32 j |
1.25 | 7.34 ± 0.03 a | 22.80 ± 0.38 h | 8.87 ± 0.07 i | 31.67 ± 0.44 i |
1.88 | 7.35 ± 0.01 a | 25.83 ± 1.03 f | 13.16 ± 0.17 h | 39.00 ± 0.88 h |
2.50 | 7.35 ± 0.04 a | 27.30 ± 0.79 e | 19.72 ± 0.03 g | 47.02 ± 0.78 f |
3.13 | 7.36 ± 0.01 a | 30.12 ± 0.12 c | 24.08 ± 0.57 f | 54.20 ± 0.65 d |
3.75 | 7.38 ± 0.03 a | 33.27 ± 0.46 b | 30.13 ± 0.25 c | 63.40 ± 0.21 b |
4.38 | 7.36 ± 0.03 a | 39.58 ± 0.34 a | 32.52 ± 0.28 b | 72.10 ± 0.45 a |
5.00 | 7.18 ± 0.17 b | 29.33 ± 1.06 cd | 32.76 ± 0.17 b | 62.09 ± 1.17 c |
5.63 | 7.17 ± 0.07 b | 28.64 ± 0.14 d | 34.13 ± 0.22 a | 62.77 ± 0.04 bc |
6.25 | 7.13 ± 0.02 b | 24.58 ± 0.23 g | 28.78 ± 0.39 d | 53.36 ± 0.50 d |
6.88 | 7.12 ± 0.02 b | 24.15 ± 0.27 g | 25.63 ± 0.02 e | 49.78 ± 0.26 e |
7.50 | 7.12 ± 0.03 b | 24.22 ± 0.34 g | 19.61 ± 0.09 g | 43.83 ± 0.26 g |
2.4. Effects of DM-301 Resin Addition and Incubation Time on Mycelial Growth and Production of Botrallin and TMC-264
Resin Addition Time (day) | Incubation Time/Harvest Time (day/day) | Mycelia Biomass (g dw/L) | Botrallin Yield (mg/L) | TMC-264 Yield (mg/L) | Yield of Botrallin Plus TMC-264 (mg/L) |
---|---|---|---|---|---|
CK | 0/20 | 6.20 ± 0.05 e | 7.26 ± 0.08 l | 3.88 ± 0.05 k | 11.14 ± 0.04 m |
0/22 | 6.87 ± 0.04 c | 17.17 ± 0.06 k | 4.85 ± 0.02 j | 22.02 ± 0.07 k | |
0/24 | 7.24 ± 0.01 a | 21.19 ± 0.02 j | 5.08 ± 0.07 j | 26.26 ± 0.05 i | |
0/26 | 7.35 ± 0.01 a | 22.97 ± 0.38 j | 5.61 ± 0.06 j | 28.58 ± 0.43 h | |
0/28 | 7.39 ± 0.01 a | 22.49 ± 0.24 i | 5.00 ± 0.18 j | 27.48 ± 0.14 hi | |
0/30 | 7.34 ± 0.01 a | 20.21 ± 0.03 j | 4.06 ± 0.07 k | 24.27 ± 0.07 j | |
0/32 | 6.93 ± 0.07 bc | 19.37 ± 0.17 j | 3.39 ± 0.03 kl | 22.76 ± 0.15 k | |
0/34 | 6.87 ± 0.19 c | 17.43 ± 0.82 k | 3.14 ± 0.23 l | 20.57 ± 0.78 l | |
0/36 | 5.69 ± 0.13 e | 17.06 ± 0.29 k | 2.81 ± 0.07 m | 19.87 ± 0.36 l | |
20 | 2/22 | 6.64 ± 0.11 d | 33.46 ± 0.99 g | 13.86 ± 0.24 i | 47.32 ± 1.17 g |
4/24 | 7.18 ± 0.15 b | 36.07 ± 0.67 f | 33.44 ± 0.11 e | 69.51 ± 0.78 e | |
6/26 | 6.93 ± 0.02 bc | 41.15 ± 0.36 de | 37.06 ± 0.58 c | 78.21 ± 0.84 c | |
8/28 | 6.91 ± 0.05 bc | 42.74±0.63 cd | 34.79±0.24 de | 77.53±0.86 c | |
10/30 | 6.92 ± 0.05 bc | 42.48 ± 0.36 cd | 35.08 ± 0.77 d | 77.56 ± 1.10 c | |
22 | 2/24 | 7.38 ± 0.02 a | 45.83 ± 0.43 c | 22.87 ± 1.10 g | 68.70 ± 1.12 e |
4/26 | 7.35 ± 0.24 a | 50.66 ± 0.36 a | 37.58 ± 0.07 c | 88.24 ± 0.30 b | |
6/28 | 7.34 ± 0.02 a | 49.86 ± 0.11 a | 37.24 ± 0.06 c | 87.11 ± 0.16 b | |
8/30 | 7.32 ± 0.01 a | 49.95 ± 0.30 a | 37.43 ± 0.10 c | 87.38 ± 0.36 b | |
10/32 | 6.98 ± 0.06 bc | 49.24 ± 0.38 ab | 36.77 ± 0.11 cd | 86.01 ± 0.42 b | |
24 | 2/26 | 7.34 ± 0.03 a | 40.82 ± 0.26 e | 27.69 ± 0.22 f | 68.50 ± 0.46 e |
4/28 | 7.55 ± 0.06 a | 51.47 ± 0.37 a | 45.85 ± 0.14 b | 97.32 ± 0.49 a | |
6/30 | 7.33 ± 0.04 a | 49.44 ± 0.10 ab | 47.74 ± 0.46 a | 97.18 ± 0.56 a | |
8/32 | 6.88 ± 0.06 c | 48.80 ± 0.47 b | 47.10 ± 0.08 a | 95.89 ± 0.48 a | |
10/34 | 6.85 ± 0.07 c | 48.88 ± 0.40 b | 47.07 ± 0.03 a | 95.95 ± 0.38 a | |
26 | 2/28 | 7.36 ± 0.04 a | 28.03 ± 1.37 i | 18.01 ± 0.12 h | 46.04 ± 1.43 g |
4/30 | 7.15 ± 0.05 b | 31.76 ± 0.83 h | 27.04 ± 0.19 f | 58.79 ± 0.95 f | |
6/32 | 6.88 ± 0.02 c | 37.27 ± 0.05 f | 36.58 ± 0.17 cd | 73.85 ± 0.12 d | |
8/34 | 6.78 ± 0.05 cd | 43.49 ± 0.15 c | 35.48 ± 0.51 d | 78.97 ± 0.65 c | |
10/36 | 5.21 ± 0.03 f | 43.68 ± 0.01 c | 35.18 ± 0.50 d | 78.85 ± 0.50 c |
3. Experimental Section
3.1. Endophytic Fungus and Culture Conditions
3.2. Macroporous Resin Selection and Preparation
Macroporous Resin | Polymer Chemistry | Polarity | Particle Size (mm) | Surface Area (m2/g) | Average pore Diameter (nm) | Moisture Content (%) |
---|---|---|---|---|---|---|
X-5 | Polystyrene | Non-polar | 0.30–1.25 | 500–600 | 29–30 | 50.13 ± 0.17 |
D-101 | Polystyrene | Non-polar | 0.30–1.25 | 480–530 | 9–11 | 67.89 ± 0.27 |
HPD-100 | Polystyrene | Non-polar | 0.30–1.25 | ≥650 | 8–9 | 68.39 ± 0.48 |
D1300 | Polystyrene | Non-polar | 0.30–1.25 | ≥600 | 9–10 | 68.27 ± 0.57 |
D3520 | Polystyrene | Non-polar | 0.30–1.25 | 480–520 | 8.5–9 | 71.56 ± 0.18 |
AB-8 | Polystyrene | Weak-polar | 0.30–1.25 | 480–520 | 13–4 | 68.33 ± 0.61 |
DM130 | Polystyrene | Weak-polar | 0.30–1.25 | 500–550 | 9–10 | 66.00 ± 0.32 |
DS-8 | Polystyrene | Weak-polar | 0.30–1.25 | 500–550 | 12–14 | 63.55 ± 0.10 |
DM-301 | Polystyrene | Middle-polar | 0.30–1.25 | ≥330 | 14–17 | 65.48 ± 1.32 |
ADS-17 | Polystyrene | Middle-polar | 0.30–1.25 | 90–150 | 25–30 | 55.16 ± 1.09 |
S-8 | Polystyrene | Polar | 0.30–1.25 | 100–200 | 28–30 | 67.12 ± 0.35 |
NKA-9 | Polystyrene | Polar | 0.30–1.25 | 250–290 | 15.5–16.5 | 70.22 ± 0.23 |
DA-201 | Polystyrene | Polar | 0.30–1.25 | ≥200 | 10–13 | 70.64 ± 0.52 |
3.3. In Situ Adsorption Culture
3.4. Analytical Procedures
3.5. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Luo, H.; Liu, H.; Cao, Y.; Xu, D.; Mao, Z.; Mou, Y.; Meng, J.; Lai, D.; Liu, Y.; Zhou, L. Enhanced Production of Botrallin and TMC-264 with in Situ Macroporous Resin Adsorption in Mycelial Liquid Culture of the Endophytic Fungus Hyalodendriella sp. Ponipodef12. Molecules 2014, 19, 14221-14234. https://doi.org/10.3390/molecules190914221
Luo H, Liu H, Cao Y, Xu D, Mao Z, Mou Y, Meng J, Lai D, Liu Y, Zhou L. Enhanced Production of Botrallin and TMC-264 with in Situ Macroporous Resin Adsorption in Mycelial Liquid Culture of the Endophytic Fungus Hyalodendriella sp. Ponipodef12. Molecules. 2014; 19(9):14221-14234. https://doi.org/10.3390/molecules190914221
Chicago/Turabian StyleLuo, Haiyu, Hongwei Liu, Yuheng Cao, Dan Xu, Ziling Mao, Yan Mou, Jiajia Meng, Daowan Lai, Yang Liu, and Ligang Zhou. 2014. "Enhanced Production of Botrallin and TMC-264 with in Situ Macroporous Resin Adsorption in Mycelial Liquid Culture of the Endophytic Fungus Hyalodendriella sp. Ponipodef12" Molecules 19, no. 9: 14221-14234. https://doi.org/10.3390/molecules190914221