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10.3390/molecules201219873
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Article

Analysis of Indole Alkaloids from Rhazya stricta Hairy Roots by Ultra-Performance Liquid Chromatography-Mass Spectrometry

by
Amir Akhgari
1,2,
Into Laakso
2,
Tuulikki Seppänen-Laakso
1,
Teijo Yrjönen
2,
Heikki Vuorela
2,
Kirsi-Marja Oksman-Caldentey
1 and
Heiko Rischer
1,*
1
VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Finland
2
Division of Pharmaceutical Biosciences, Faculty of Pharmacy, P.O. Box 56, FI-00014 University of Helsinki, Finland
*
Author to whom correspondence should be addressed.
Molecules 2015, 20(12), 22621-22634; https://doi.org/10.3390/molecules201219873
Submission received: 28 October 2015 / Revised: 10 December 2015 / Accepted: 11 December 2015 / Published: 17 December 2015
(This article belongs to the Section Natural Products Chemistry)
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Abstract

Rhazya stricta Decne. (Apocynaceae) contains a large number of terpenoid indole alkaloids (TIAs). This study focused on the composition of alkaloids obtained from transformed hairy root cultures of R. stricta employing ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). In the UPLC-MS analyses, a total of 20 TIAs were identified from crude extracts. Eburenine and vincanine were the main alkaloids followed by polar glucoalkaloids, strictosidine lactam and strictosidine. Secodine-type alkaloids, tetrahydrosecodinol, tetrahydro- and dihydrosecodine were detected too. The occurrence of tetrahydrosecodinol was confirmed for the first time for R. stricta. Furthermore, two isomers of yohimbine, serpentine and vallesiachotamine were identified. The study shows that a characteristic pattern of biosynthetically related TIAs can be monitored in Rhazya hairy root crude extract by this chromatographic method.
Keywords: Rhazya stricta; terpenoid indole alkaloids; ultra-performance liquid chromatography-mass spectrometry Rhazya stricta; terpenoid indole alkaloids; ultra-performance liquid chromatography-mass spectrometry
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MDPI and ACS Style

Akhgari, A.; Laakso, I.; Seppänen-Laakso, T.; Yrjönen, T.; Vuorela, H.; Oksman-Caldentey, K.-M.; Rischer, H. Analysis of Indole Alkaloids from Rhazya stricta Hairy Roots by Ultra-Performance Liquid Chromatography-Mass Spectrometry. Molecules 2015, 20, 22621-22634. https://doi.org/10.3390/molecules201219873

AMA Style

Akhgari A, Laakso I, Seppänen-Laakso T, Yrjönen T, Vuorela H, Oksman-Caldentey K-M, Rischer H. Analysis of Indole Alkaloids from Rhazya stricta Hairy Roots by Ultra-Performance Liquid Chromatography-Mass Spectrometry. Molecules. 2015; 20(12):22621-22634. https://doi.org/10.3390/molecules201219873

Chicago/Turabian Style

Akhgari, Amir, Into Laakso, Tuulikki Seppänen-Laakso, Teijo Yrjönen, Heikki Vuorela, Kirsi-Marja Oksman-Caldentey, and Heiko Rischer. 2015. "Analysis of Indole Alkaloids from Rhazya stricta Hairy Roots by Ultra-Performance Liquid Chromatography-Mass Spectrometry" Molecules 20, no. 12: 22621-22634. https://doi.org/10.3390/molecules201219873

APA Style

Akhgari, A., Laakso, I., Seppänen-Laakso, T., Yrjönen, T., Vuorela, H., Oksman-Caldentey, K.-M., & Rischer, H. (2015). Analysis of Indole Alkaloids from Rhazya stricta Hairy Roots by Ultra-Performance Liquid Chromatography-Mass Spectrometry. Molecules, 20(12), 22621-22634. https://doi.org/10.3390/molecules201219873

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