Culture Condition Optimization and Pilot Scale Production of the M12 Metalloprotease Myroilysin Produced by the Deep-Sea Bacterium Myroides profundi D25
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effects of Culture Temperature and Inoculation Amount on the Production of Myroilysin
2.2. Optimization of the Contents of Carbon Source and Nitrogen Source in the Medium
2.3. Protease Production of D25 under the Optimal Conditions
2.4. Small Scale Fermentation of D25
2.5. Pilot Scale Fermentation of D25
3. Experimental Section
3.1. Strain and Media
3.2. Inoculum Preparation and Flask Fermentation
3.3. Assay of the Protease Activity
3.4. Optimization by Single Factor Experiments
3.5. Small Scale Fermentation and Pilot Scale Fermentation
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Shao, X.; Ran, L.-Y.; Liu, C.; Chen, X.-L.; Zhang, X.-Y.; Qin, Q.-L.; Zhou, B.-C.; Zhang, Y.-Z. Culture Condition Optimization and Pilot Scale Production of the M12 Metalloprotease Myroilysin Produced by the Deep-Sea Bacterium Myroides profundi D25. Molecules 2015, 20, 11891-11901. https://doi.org/10.3390/molecules200711891
Shao X, Ran L-Y, Liu C, Chen X-L, Zhang X-Y, Qin Q-L, Zhou B-C, Zhang Y-Z. Culture Condition Optimization and Pilot Scale Production of the M12 Metalloprotease Myroilysin Produced by the Deep-Sea Bacterium Myroides profundi D25. Molecules. 2015; 20(7):11891-11901. https://doi.org/10.3390/molecules200711891
Chicago/Turabian StyleShao, Xuan, Li-Yuan Ran, Chang Liu, Xiu-Lan Chen, Xi-Ying Zhang, Qi-Long Qin, Bai-Cheng Zhou, and Yu-Zhong Zhang. 2015. "Culture Condition Optimization and Pilot Scale Production of the M12 Metalloprotease Myroilysin Produced by the Deep-Sea Bacterium Myroides profundi D25" Molecules 20, no. 7: 11891-11901. https://doi.org/10.3390/molecules200711891