Biofunctional Constituents from Michelia compressa var. lanyuensis with Anti-Melanogenic Properties
Abstract
:1. Introduction
2. Results and Discussion
2.1. Anti-Proliferative Properties of Compounds 1–7 from M. compressa var. lanyuensis on B16F10 Cells
2.2. Inhibitory Effects of the Test Compounds on Tyrosinase Activity and Melanin Content
Compound | Inhibition (%) Tyrosinase Activity | Inhibition (%) Melanin Content |
---|---|---|
Liriodenine (1) (5 μM) | 14.52 ± 3.58 | 19.03 ± 4.55 |
(−)-N-Acetylanonaine (2) (10 μM) | 7.75 ± 1.42 | 17.70 ± 3.18 |
Pressalanine A (3) (10 μM) | 4.84 ± 0.80 | −12 ± 4.58 |
p-Dihydroxybenzaldehyde (4) (10 μM) | −7.77 ± 1.80 | −7.90 ± 2.22 |
3,4-Dihydroxybenzoic acid (5) (5 μM) | −28.28 ± 7.35 | −18.37 ± 6.43 |
(−)-Bornesitol (6) (5 μM) | 3.06 ± 0.45 | 0.89 ± 0.08 |
β-Sitostenone (7) (5 μM) | 25.48 ± 6.30 | 35.86 ± 5.43 |
Kojic acid (10 μM) | 25.38 ± 1.40 | 20.00 ± 2.12 |
3. Experimental Section
3.1. General Procedures
3.2. Plant Material
3.3. Extraction, Isolation and Identification
3.4. Cell Culture
3.5. Cell Viability Assay—XTT Assay
3.6. Determination of Melanin Content
3.7. Tyrosinase Activity
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Chu, C.-W.; Liu, C.-M.; Chung, M.-I.; Chen, C.-Y. Biofunctional Constituents from Michelia compressa var. lanyuensis with Anti-Melanogenic Properties. Molecules 2015, 20, 12166-12174. https://doi.org/10.3390/molecules200712166
Chu C-W, Liu C-M, Chung M-I, Chen C-Y. Biofunctional Constituents from Michelia compressa var. lanyuensis with Anti-Melanogenic Properties. Molecules. 2015; 20(7):12166-12174. https://doi.org/10.3390/molecules200712166
Chicago/Turabian StyleChu, Chia-Wei, Chi-Ming Liu, Mei-Ing Chung, and Chung-Yi Chen. 2015. "Biofunctional Constituents from Michelia compressa var. lanyuensis with Anti-Melanogenic Properties" Molecules 20, no. 7: 12166-12174. https://doi.org/10.3390/molecules200712166