Regulation of the scp Genes in the Cyanobacterium Synechocystis sp. PCC 6803—What is New?
Abstract
:1. Introduction
2. Results and Discussion
2.1. NtcA Binds to the Promoter Regions of scpB and scpE
2.2. 2-Oxoglutarate does not Stimulate NtcA Binding
2.3. scpB and scpC Contain a HIP1 Motif
2.4. HLR1 Motif
Gene ID | Gene Product | HLR1 | p-Value | Position Relative to the Translational Start Site | Strand |
---|---|---|---|---|---|
ssl2542 * | ScpB | TTACAATAGTTAACAT | 1.16 × 10−8 | −42 | + |
sll1483 | Hypothetical protein | TTCACAAAAATTTATA | 1.41 × 10−8 | −63 | + |
sll0157 | Hypothetical protein | TTACAATCGTTTACAA | 1.54 × 10−8 | −50 | + |
sll2012 | SigD | TGAGAAAACTTTACAA | 6.38 × 10−8 | −44 | − |
slr1894 | MrgA | TAACAAAATTTAACAC | 1.97 × 10−8 | −32 | − |
slr0320 | Hypothetical protein | TTAAGATATATTACAA | 6.57 × 10−8 | −78 | + |
slr1687 | Hypothetical protein | TAACAAAACTTTATAA | 1.07 × 10−8 | −47 | − |
ssl3044 | Probable ferredoxin | TAACAAAACTTTATAA | 1.07 × 10−8 | −142 | + |
ssl2162 | Unknown protein | TGTAAACCTTTGTTAA | 1.13 × 10−8 | −75 | − |
3. Experimental Section
3.1. Strains and Growth Conditions
3.2. Protein Expression and Purification
3.3. Electrophoretic Mobility Shift Assay (EMSA)
NtcA-Fw | 5ʹ-AACGTCCATGGATCAGTCCCTA-3ʹ |
NtcA-R | 5ʹ-CATAGAGGTACCTTAGGTAAACTG-3ʹ |
Sll1130-Fw | 5ʹ-GCGCCATGGATACAATTTACGA ACAATTTG-3ʹ |
Sll1130-R | 5ʹ-GCGAAGCTTACCGAGTTTAAAAACATGGGG-3′ |
scpB-Fw | 5ʹ-TTTGAGCCTAACATTATCCTCC-3ʹ |
scpB-R | 5ʹ-ATGTAATTACGAGCTGGGAGG-3ʹ |
scpE-Fw | 5ʹ-ACTCTGACGTACCCATTGAC-3ʹ |
scpE-R | 5ʹ-CTCATGGGATTTCCTGGGATA-3ʹ |
scpB-CR-Fw | 5ʹ-TAAATTTGGATTCACTGCTTTCG-3ʹ |
scpB-CR-R | 5ʹ-AGTGAAGTACACCTTGCCCAGAG-3ʹ |
Fw-HIP1 | 5ʹ-ATCCTGGGACTGGAGAATCA-3ʹ |
R-HIP1 | 5ʹ-CGGGTGGTCATAATTGGATTA-3ʹ |
3.4. Bioinformatic Analysis
3.5. DNA Pull down Assay
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Cheregi, O.; Funk, C. Regulation of the scp Genes in the Cyanobacterium Synechocystis sp. PCC 6803—What is New? Molecules 2015, 20, 14621-14637. https://doi.org/10.3390/molecules200814621
Cheregi O, Funk C. Regulation of the scp Genes in the Cyanobacterium Synechocystis sp. PCC 6803—What is New? Molecules. 2015; 20(8):14621-14637. https://doi.org/10.3390/molecules200814621
Chicago/Turabian StyleCheregi, Otilia, and Christiane Funk. 2015. "Regulation of the scp Genes in the Cyanobacterium Synechocystis sp. PCC 6803—What is New?" Molecules 20, no. 8: 14621-14637. https://doi.org/10.3390/molecules200814621