Comparative Incorporation of PNA into DNA Nanostructures
Abstract
:1. Introduction
2. Results and Discussion
2.1. Melting Curves
MgCl2 Concentration | DNA·DNA | PNA3K·DNA | ΔTm |
---|---|---|---|
1 mM | 40 | 78 | 38 |
6 mM | 56 | 77 | 21 |
12.5 mM | 57 | 76 | 19 |
2.2. Duplex Invasion
1 mM MgCl2 | 6 mM MgCl2 | 12.5 mM MgCl2 | |
---|---|---|---|
k (s−1∙M−1) | 0.0025 | 0.00072 | 0.00034 |
2.3. Binding of PNA to DNA Origami
bPNA3K | DNA | |
---|---|---|
Origami | 596 | 883 |
Center site | 510 | 5 |
Side site | 322 | 458 |
Corner site | 344 | 276 |
3. Experimental Section
3.1. PNA Synthesis
3.2. UV-Vis Melting Curves
3.3. Nanostructure Assembly
3.4. Atomic Force Microscopy
3.5. Fluorescence
4. Conclusions
Supplementary Material
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Pedersen, R.O.; Kong, J.; Achim, C.; LaBean, T.H. Comparative Incorporation of PNA into DNA Nanostructures. Molecules 2015, 20, 17645-17658. https://doi.org/10.3390/molecules200917645
Pedersen RO, Kong J, Achim C, LaBean TH. Comparative Incorporation of PNA into DNA Nanostructures. Molecules. 2015; 20(9):17645-17658. https://doi.org/10.3390/molecules200917645
Chicago/Turabian StylePedersen, Ronnie O., Jing Kong, Catalina Achim, and Thomas H. LaBean. 2015. "Comparative Incorporation of PNA into DNA Nanostructures" Molecules 20, no. 9: 17645-17658. https://doi.org/10.3390/molecules200917645