Cordycepin Induces Apoptosis and Inhibits Proliferation of Human Lung Cancer Cell Line H1975 via Inhibiting the Phosphorylation of EGFR
Abstract
:1. Introduction
2. Results and Discussion
2.1. Cordycepin Extraction and Purification
2.2. Cordycepin Inhibits H1975 Cell Proliferation
2.3. Cordycepin Induces G0/G1 Phase Arrest in H1975 Cells
2.4. Cordycepin Induces Apoptosis and Regulates Apoptosis-Related Proteins Bcl-2 and Caspase-3 in H1975 Cells
2.5. Cordycepin Inhibits the Phosphorylation of EGFR, AKT and ERK1/2 in H1975 Cells
3. Experimental Section
3.1. Cordycepin Extraction and Purification
3.2. Cell Culture and MTT Assay
3.3. Flow Cytometry Analysis
3.4. Western Blot Analysis
3.5. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Wang, Z.; Wu, X.; Liang, Y.-N.; Wang, L.; Song, Z.-X.; Liu, J.-L.; Tang, Z.-S. Cordycepin Induces Apoptosis and Inhibits Proliferation of Human Lung Cancer Cell Line H1975 via Inhibiting the Phosphorylation of EGFR. Molecules 2016, 21, 1267. https://doi.org/10.3390/molecules21101267
Wang Z, Wu X, Liang Y-N, Wang L, Song Z-X, Liu J-L, Tang Z-S. Cordycepin Induces Apoptosis and Inhibits Proliferation of Human Lung Cancer Cell Line H1975 via Inhibiting the Phosphorylation of EGFR. Molecules. 2016; 21(10):1267. https://doi.org/10.3390/molecules21101267
Chicago/Turabian StyleWang, Zheng, Xue Wu, Yan-Ni Liang, Li Wang, Zhong-Xing Song, Jian-Li Liu, and Zhi-Shu Tang. 2016. "Cordycepin Induces Apoptosis and Inhibits Proliferation of Human Lung Cancer Cell Line H1975 via Inhibiting the Phosphorylation of EGFR" Molecules 21, no. 10: 1267. https://doi.org/10.3390/molecules21101267