Interaction of Di-2-pyridylketone 2-pyridine Carboxylic Acid Hydrazone and Its Copper Complex with BSA: Effect on Antitumor Activity as Revealed by Spectroscopic Studies
Abstract
:1. Introduction
2. Results and Discussion
2.1. BSA Mediated Cytotoxicity of DPPCAH and Its Copper Complex (DPPCAH-Cu)
2.2. Interactions of DPPCAH and DPPCAH-Cu with BSA
2.3. Binding Sites and Binding Constants
2.4. Binding Sites and Binding Constants
2.5. Binding Forces
2.6. Energy Transfer between DPPCAH (DPPCAH-Cu) and BSA
2.7. Conformational Investigation by Synchronous Fluorescence
2.8. Circular Dichroism Spectra
2.9. Molecular Docking
3. Materials and Methods
3.1. General Information
3.2. Cytotoxicity Assay (MTT Assay)
3.3. UV–Vis Spectrophotometric Measurements
3.4. Fluorescence Measurements
3.5. Measurements of CD Spectra
3.6. Molecular Docking
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
BSA | Bovine serum albumin |
DPPCAH | Di-2-pyridylketone 2-pyridinecarboxylic acid hydrazone |
DPPCAH-Cu | Copper complex of di-2-pyridylketone 2-pyridinecarboxylic acid hydrazone |
FRET | Fluorescence resonance energy transfer |
References
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System | Δ𝐻 (kJ·mol−1) | ΔS J/mol·K | ΔG (kJ·mol−1) |
---|---|---|---|
BSA-DPPCAH | −46.97 | −60.0 | −29.69 |
BSA-DPPCAH-Cu | −38.57 | −41.5 | −26.61 |
System | J (cm3·L·mol−1) | E (%) | R0 (nm) | r (nm) |
---|---|---|---|---|
BSA-DPPCAH | 7.424 × 1013 | 0.297 | 2.35 | 2.04 |
BSA-DPPCAH-Cu | 1.699 × 1014 | 0.288 | 2.70 | 2.32 |
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Li, C.; Huang, T.; Fu, Y.; Liu, Y.; Zhou, S.; Qi, Z.; Li, C. Interaction of Di-2-pyridylketone 2-pyridine Carboxylic Acid Hydrazone and Its Copper Complex with BSA: Effect on Antitumor Activity as Revealed by Spectroscopic Studies. Molecules 2016, 21, 563. https://doi.org/10.3390/molecules21050563
Li C, Huang T, Fu Y, Liu Y, Zhou S, Qi Z, Li C. Interaction of Di-2-pyridylketone 2-pyridine Carboxylic Acid Hydrazone and Its Copper Complex with BSA: Effect on Antitumor Activity as Revealed by Spectroscopic Studies. Molecules. 2016; 21(5):563. https://doi.org/10.3390/molecules21050563
Chicago/Turabian StyleLi, Cuiping, Tengfei Huang, Yun Fu, Youxun Liu, Sufeng Zhou, Zhangyang Qi, and Changzheng Li. 2016. "Interaction of Di-2-pyridylketone 2-pyridine Carboxylic Acid Hydrazone and Its Copper Complex with BSA: Effect on Antitumor Activity as Revealed by Spectroscopic Studies" Molecules 21, no. 5: 563. https://doi.org/10.3390/molecules21050563