Soy Isoflavones Regulate Lipid Metabolism through an AKT/mTORC1 Pathway in Diet-Induced Obesity (DIO) Male Rats
Abstract
:1. Introduction
2. Results
2.1. Soy Isoflavones Exhibited Remarkable Effects on Body Weight and Adiposity in DIO Male Rats
2.2. Soy Isoflavones Could Reduce Lipid Accumulation in Livers of DIO Rats
2.3. Effects of Soy Isoflavones on the Lipid Metabolism in the Liver and Adipose Tissue
2.4. Soy Isoflavones Suppressed the Activity of mTORC1
3. Discussion
4. Materials and Methods
4.1. Animal Care and Maintenance
4.2. Body Weight, Food Intake, and Plasma Measurements
4.3. Histopathologic Evaluation
4.4. OA/BSA Complex Solution Preparation
4.5. Cell Culture
4.6. Quantitative Realtime PCR
4.7. Cell Viability Assay
4.8. Western Blotting
4.9. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
DIO | diet induced obesity |
mTORC1 | mechanistic target of rapamycin complexes 1 |
Ctr | Control |
H and E staining | hematoxylin and eosin staining |
OA | oleic acid |
OB | obesity control group |
LSI | low dose of soy isoflavones |
MSI | middle dose of soy isoflavones |
HSI | high dose of soy isoflavones |
TG | triglycerides |
LDL | low-density lipoprotein |
SREBP1 | Sterol regulatory element-binding transcription factor 1 |
ACC | Acetyl-CoA carboxylase |
ACL | ATP citrate lyase |
FASN | Fatty acid synthase |
ACSL | Long chain fatty acid-CoA ligase |
ATGL | Adipose triglyceride lipase |
HSL | Hormone sensitive lipase |
PPARs | peroxisome proliferator-activated receptors |
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Ingredients | Content |
---|---|
Corn | 54.0% |
Fish meal | 6.0% |
Wheat bran | 14.0% |
Alfalfa meal | 13.0% |
Cotton meal | 10.0% |
Limestone | 1.00% |
Dicalcium phosphate | 0.2% |
Dodium chloride | 0.3% |
Vitamin & mineral | 1.5% |
Groups | Control Groups (Ctr, n = 10) | Obesity Groups (OB, n = 15) | Low-Dose Soy Isoflavones (LSI, n = 9) | Middle-Dose Soy Isoflavones (MSI, n = 15) | High-Dose Soy Isoflavones (HIS, n = 15) |
---|---|---|---|---|---|
Diets | Basal diets | Basal diets | Basal diets + 50 mg/kg soy isoflavones | Basal diets + 150 mg/kg soy isoflavones | Basal diets + 400 mg/kg soy isoflavones |
Compounds | Content |
---|---|
Daidzin | 50.98% |
Glycitin | 30.36% |
Genistein | 8.80% |
Daidzein | 1.24% |
Genistin | 0.06% |
Total isoflavones | 91.64% |
Gene | Primers |
---|---|
SREBP-1c | Fr 5’-TGGACTACTAGTGTTGGCCTGCTT-3’ |
Rv 5’-ATCCAGGTCAGCTTGTTTGCGATG-3’ | |
ACC-1 | Fr 5’-ATTGTGGCTCAAACTGCAGGT-3’ |
Rv 5’-GCCAATCCACTCGAAGACCA-3’ | |
ACC-2 | Fr 5’-CAACATCCGTCAGACGACCTC-3’ |
Rv 5’-CGGACTCGTTGGTGATGAAGA-3’ | |
FASN | Fr 5’-TCCCAGGTCTTGCCGTGC-3’ |
Rv 5’-GCGGATGCCTAGGATGTGTGC-3’ | |
ACSL1 | Fr 5’-GGTGCTTCAGCCTACCATCTTCC-3’ |
Rv 5’-AATCCAACAGCCATCGCTTCACT-3’ | |
ACSL4 | Fr 5’-TATGGGCTGACAGAATCATG-3’ |
Rv 5’-CAACTCTTCCAGTAGTGTAG-3’ | |
PPARα | Fr 5’-ACTCGCAGGAAAGACTAGCA-3’ |
Rv 5’-AGCAGTGGAAGAATCGGACC-3’ | |
HSL | Fr 5’-TCAGGTGTCTTTGCGGGTAT-3’ |
Rv 5’-CTTGTGCGGAAGAAGATGCT-3’ | |
ATGL | Fr 5’-TCACCAACACCAGCATCCA-3’ |
Rv 5’-GCACATCTCTCGAAGCACCA-3’ | |
PPARγ | Fr 5’-CATTCGCATCTTTCAGGG-3’ |
Rv 5’-GGACGCCATACTTTAGGA-3’ | |
ACL | Fr 5’-GCAGCACGTGATCCATGAAT-3’ |
Rv 5’-GTGGGATGCTGGACAACATC-3’ | |
β-Actin | Fr 5’-GTACCACTGGCATTGTGATG-3’ |
Rv 5’-ATCTTCATGGTGCTAGGAGC-3’ |
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Share and Cite
Huang, C.; Pang, D.; Luo, Q.; Chen, X.; Gao, Q.; Shi, L.; Liu, W.; Zou, Y.; Li, L.; Chen, Z. Soy Isoflavones Regulate Lipid Metabolism through an AKT/mTORC1 Pathway in Diet-Induced Obesity (DIO) Male Rats. Molecules 2016, 21, 586. https://doi.org/10.3390/molecules21050586
Huang C, Pang D, Luo Q, Chen X, Gao Q, Shi L, Liu W, Zou Y, Li L, Chen Z. Soy Isoflavones Regulate Lipid Metabolism through an AKT/mTORC1 Pathway in Diet-Induced Obesity (DIO) Male Rats. Molecules. 2016; 21(5):586. https://doi.org/10.3390/molecules21050586
Chicago/Turabian StyleHuang, Chao, Dejiang Pang, Qihui Luo, Xiaolin Chen, Qi Gao, Liangqin Shi, Wentao Liu, Yuanfeng Zou, Lixia Li, and Zhengli Chen. 2016. "Soy Isoflavones Regulate Lipid Metabolism through an AKT/mTORC1 Pathway in Diet-Induced Obesity (DIO) Male Rats" Molecules 21, no. 5: 586. https://doi.org/10.3390/molecules21050586