In Situ Enzymatically Generated Photoswitchable Oxidase Mimetics and Their Application for Colorimetric Detection of Glucose Oxidase
Abstract
:1. Introduction
2. Results and Discussion
2.1. The Photoswitchable Oxidase Mimetics of PO43−-Capped CdS QDs Generated by GOx-Mediated Biocatalysis
2.2. Mechanism of Photoswitchable Enzyme-Like Activity of PO43−-Capped CdS QDs
2.3. Probing the Activity of Glucose Oxidase Using PO43−-Capped CdS QDs
3. Materials and Methods
3.1. Chemicals and Materials
3.2. Instrumentation
3.3. The Colorimetric Detection of Glucose Oxidase Activity
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
GOx | glucose oxidase |
CdS QDs | cadmium sulfide quantum dots |
TMB | 3,3′,5,5′-tetramethylbenzydine |
NPs | Nanoparticles |
h+ | photo-generated holes |
•OH | hydroxyl radical |
O2•− | superoxide anion |
HRP | horseradish peroxidase |
SOD | superoxide dismutase |
CAT | Catalase |
PDDA | poly dimethyl diallyl ammonium chloride |
TGA | thioglycolic acid |
CS | Chitosan |
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- Sample Availability: Samples of the compounds are available from the authors.
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Cao, G.-X.; Wu, X.-M.; Dong, Y.-M.; Li, Z.-J.; Wang, G.-L. In Situ Enzymatically Generated Photoswitchable Oxidase Mimetics and Their Application for Colorimetric Detection of Glucose Oxidase. Molecules 2016, 21, 902. https://doi.org/10.3390/molecules21070902
Cao G-X, Wu X-M, Dong Y-M, Li Z-J, Wang G-L. In Situ Enzymatically Generated Photoswitchable Oxidase Mimetics and Their Application for Colorimetric Detection of Glucose Oxidase. Molecules. 2016; 21(7):902. https://doi.org/10.3390/molecules21070902
Chicago/Turabian StyleCao, Gen-Xia, Xiu-Ming Wu, Yu-Ming Dong, Zai-Jun Li, and Guang-Li Wang. 2016. "In Situ Enzymatically Generated Photoswitchable Oxidase Mimetics and Their Application for Colorimetric Detection of Glucose Oxidase" Molecules 21, no. 7: 902. https://doi.org/10.3390/molecules21070902