Influence of Cysteine and Tryptophan Substitution on DNA-Binding Activity on Maize α-Hairpinin Antimicrobial Peptide
Abstract
:1. Introduction
2. Results
2.1. Synthesis, Folding, Purification and Activity of MBP-1
2.2. Design and Activity of MBP-1 Variants
2.3. Scanning Electron Microscopy
2.4. DNA Binding-Assay
2.5. Structural Analysis
3. Discussion
4. Materials and Methods
4.1. Synthesis, Purification and Folding of MBP-1 and Variants
4.2. Antibacterial Bioassays
4.3. Scanning Electron Microscopy
4.4. DNA Binding-Assay
4.5. Comparative Molecular Modelling
4.6. Molecular Dynamics Simulation and Analyses of Molecular Dynamics Trajectories
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Peptide | Sequence | Mass before Oxidation | Mass after Oxidation | MIC against E. coli |
---|---|---|---|---|
MBP-1 | RSGRGECRRQCLRRHEGQPWETQECMRRCRRRG | 4125.69 m/z | 4121.04 m/z | 50 μM |
Var 1 | RSGRGECRRQCLRRHEGQPAETQECMRRCRRRG | 4008.05 m/z | 4004.01 m/z | >400 μM |
Var 2 | RSGRGEARRQALRRHEGQPWETQEAMRRARRRG | 4001.40 m/z | 4001.38 m/z | >400 μM |
Peptide | Solvation Potential Energy (KJ/mol) |
---|---|
MBP-1 | 3399.486 |
Var 1 | 3373.256 |
Var 2 | 3487.306 |
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Sousa, D.A.; Porto, W.F.; Silva, M.Z.; Da Silva, T.R.; Franco, O.L. Influence of Cysteine and Tryptophan Substitution on DNA-Binding Activity on Maize α-Hairpinin Antimicrobial Peptide. Molecules 2016, 21, 1062. https://doi.org/10.3390/molecules21081062
Sousa DA, Porto WF, Silva MZ, Da Silva TR, Franco OL. Influence of Cysteine and Tryptophan Substitution on DNA-Binding Activity on Maize α-Hairpinin Antimicrobial Peptide. Molecules. 2016; 21(8):1062. https://doi.org/10.3390/molecules21081062
Chicago/Turabian StyleSousa, Daniel A., William F. Porto, Maria Z. Silva, Tatiane R. Da Silva, and Octávio L. Franco. 2016. "Influence of Cysteine and Tryptophan Substitution on DNA-Binding Activity on Maize α-Hairpinin Antimicrobial Peptide" Molecules 21, no. 8: 1062. https://doi.org/10.3390/molecules21081062