Cytotoxicity of Triterpenoid Alkaloids from Buxus microphylla against Human Tumor Cell Lines
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
3.1. General Information
3.2. Plant Material
3.3. Extraction and Isolation
3.4. Cytotoxicity Assay
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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- Sample Availability: Samples of the compounds 1 and 3 are available from the authors.
No. | 1 | 2 | 3 | |||
---|---|---|---|---|---|---|
δC, Type | δH | δC, Type | δH | δC, Type | δH | |
1 | 33.0, CH2 | 1.26 m; 1.63 m | 32.9, CH2 | 1.28 m; 1.61 m | 33.0, CH2 | 1.29 m; 1.65 m |
2 | 27.4, CH2 | 1.43 m; 1.53 m | 27.1, CH2 | 1.42 m; 1.55 m | 27.4, CH2 | 1.46 m; 1.57 m |
3 | 63.3, CH | 2.60 m | 63.2, CH | 2.60 m | 63.3, CH | 2.60 m |
4 | 37.1, C | - | 37.0, C | - | 37.1, C | - |
5 | 45.0, CH | 1.35 m | 44.8, CH | 1.35 m | 44.8, CH | 1.35 m |
6 | 19.9, CH2 | 0.79 m; 1.29 m | 19.7, CH2 | 1.84 m; 1.25 m | 19.8, CH2 | 0.81 m; 1.26 m |
7 | 25.3, CH2 | 1.11 m; 1.29 m | 25.1, CH2 | 1.02 m; 1.25 m | 25.2, CH2 | 1.05 m; 1.25 m |
8 | 47.3, CH | 1.51 m | 46.7, CH | 1.60 m | 46.8, CH | 1.57 m |
9 | 19.1, C | - | 18.9, C | - | 19.0, C | - |
10 | 25.3, C | - | 25.7, C | - | 25.8, C | - |
11 | 25.9, CH2 | 1.10 m; 2.00 m | 26.0, CH2 | 1.11 m; 1.99 m | 26.1, CH2 | 1.10 m; 2.01 m |
12 | 31.5, CH2 | 1.45 m; 1.61 m | 32.1, CH2 | 1.58 m; 1.72 m | 32.3, CH2 | 1.59 m; 1.74 m |
13 | 44.8, C | - | 44.7, C | - | 44.9, C | - |
14 | 47.3, C | - | 47.7, C | - | 47.8, C | - |
15 | 44.4, CH2 | 1.34 m; 1.85 m | 44.5, CH2 | 1.41 m; 1.95 m | 44.5, CH2 | 1.42 m; 1.98 m |
16 | 78.9, CH | 4.05 m | 80.4, CH | 5.26 m | 80.3, CH | 5.30 m |
17 | 56.9, CH | 1.84 m | 56.4, CH | 2.27 m | 56.6, CH | 2.29 m |
18 | 18.7, CH3 | 0.96 s | 18.8, CH3 | 1.00 s | 18.9, CH3 | 1.03 s |
19 | 30.7, CH2 | 0.59 d (4.0) 0.33 d (4.0) | 30.2, CH2 | 0.60 brs 0.33 d brs | 30.4, CH2 | 0.63 d (4.0) 0.36 d (4.0) |
20 | 62.4, CH | 2.63 m | 59.7, CH | 2.55 m | 59.8, CH | 2.57 m |
21 | 9.6, CH3 | 0.87 d (6.4) | 9.4, CH3 | 0.84 d (5.8) | 9.6, CH3 | 0.86 d (6.4) |
30 | 77.3, CH2 | 3.74 d (10.7) 3.31 d (10.7) | 77.2, CH2 | 3.73 d (10.9) 3.28 d (10.9) | 77.3, CH2 | 3.75 d (10.7) 3.31 d (10.7) |
31 | 11.4, CH3 | 0.98 s | 11.3, CH3 | 0.97 s | 11.6, CH3 | 0.99 s |
32 | 20.9, CH3 | 1.12 s | 19.5, CH3 | 1.11 s | 19.5, CH3 | 1.14 s |
1’ | 85.3, CH | 4.29 m | 85.1, CH | 4.28 m | 85.3, CH | 4.28 m |
2’ | 21.7, CH3 | 1.30 d (5.5) | 21.5, CH3 | 1.28 d (5.4) | 21.7, CH3 | 1.31 d (5.4) |
N(CH3)2 | 40.1, CH3 | 2.09 s | 40.4, CH3 | 2.10 s | 40.3, CH3 | 2.10 s |
OCH3 | - | - | 56.2, CH3 | 3.88 s | 56.0, CH3 | 3.93 s |
OSyr b (OVan) b | - | - | 165.8, C 121. 8, C 106.5, CH 146.7, C 139.1, C | 7.27 s | 165.8, C 123.4, C 111.8, CH 149.5, C 146.1, C 113.8, CH 123.7, CH | 7.60 brdd 7.57 brd 6.91 d (8.3) |
Compounds | Cell Lines | ||||
---|---|---|---|---|---|
HL-60 | SMMC-7721 | A-549 | MCF-7 | SW480 | |
1 | >40 | >40 | >40 | >40 | >40 |
2 | >40 | >40 | >40 | >40 | >40 |
3 | 13.88 | 15.58 | 11.76 | 4.51 | 13.71 |
Cisplatin | 1.22 | 4.48 | 6.18 | 15.23 | 11.99 |
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Bai, S.-T.; Zhu, G.-L.; Peng, X.-R.; Dong, J.-R.; Yu, M.-Y.; Chen, J.-C.; Wan, L.-S.; Qiu, M.-H. Cytotoxicity of Triterpenoid Alkaloids from Buxus microphylla against Human Tumor Cell Lines. Molecules 2016, 21, 1125. https://doi.org/10.3390/molecules21091125
Bai S-T, Zhu G-L, Peng X-R, Dong J-R, Yu M-Y, Chen J-C, Wan L-S, Qiu M-H. Cytotoxicity of Triterpenoid Alkaloids from Buxus microphylla against Human Tumor Cell Lines. Molecules. 2016; 21(9):1125. https://doi.org/10.3390/molecules21091125
Chicago/Turabian StyleBai, Shi-Tou, Guo-Lei Zhu, Xing-Rong Peng, Jin-Run Dong, Mu-Yuan Yu, Jian-Chao Chen, Luo-Sheng Wan, and Ming-Hua Qiu. 2016. "Cytotoxicity of Triterpenoid Alkaloids from Buxus microphylla against Human Tumor Cell Lines" Molecules 21, no. 9: 1125. https://doi.org/10.3390/molecules21091125