Metabolic Profile of Skimmianine in Rats Determined by Ultra-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry
Abstract
:1. Introduction
2. Results and Discussion
2.1. Mass Spectral Properities of Skimmianine
2.2. Structure Elucidation of Skimmianine Metabolites
2.3. Metabolic Profile of Skimmianinein Rats
3. Materials and Methods
3.1. Chemical, Reagents, and Materials
3.2. Instrumentation and Analytical Conditions
3.3. Animals, Dosage and Biological Sample Collection
3.4. Biological Sample Preparation
3.5. Data Processing
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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NO. | RT (min) | Mass Found | Error (ppm) | Formula [M + H]+ | MS2 Ions | Source | Metabolite Name |
---|---|---|---|---|---|---|---|
M0 | 15.5 | 260.0919 | 0.7 | C14H14NO4 | 245.0681, 244.0602, 227.0575, 216.0652, 199.0633, 184.0399 | P U F | Parent |
M1 | 12.3 | 276.0864 | −0.8 | C14H14NO5 | 248.0918, 233.0679, 232.0608, 217.0735, 216.0659, 204.0655, 188.0708 | P U | 2,3-epoxide of skimmianine |
M2 | 6.9 | 276.0874 | 2.7 | C14H14NO5 | 261.0627, 243.0527, 215.0565, 200.0333 | U | C5-hydroxylation of skimmianine |
M3 | 13.3 | 276.0861 | −2.0 | C14H14NO5 | 261.0627, 243.0527, 215.0565, 200.0333 | U | C6-hydroxylation of skimmianine |
M4 | 12.3 | 294.0976 | 1.2 | C14H16NO6 | 276.0862, 248.0918, 217.0735, 216.0659, 188.0708, 175.0618 | P U F | 2,3-epoxide hydrolysis of M1 |
M5 | 9.9, | 246.0763 | 0.8 | C13H12NO4 | 231.0531, 213.0419, 185.0470 | P U F | C4-O-demethylation of skimmianine |
M6 | 11.7 | 246.0761 | 0 | C13H12NO4 | 231.0526, 216.0289, 188.0342, 160.0388 | P U | C8-O-demethylation of skimmianine |
M7 | 13.1 | 246.0760 | −0.2 | C13H12NO4 | 231.0511, 216.0274, 188.0327, 160.0384 | P U F | C7-O-demethylation of skimmianine |
M8 | 7.7 | 422.1079 | −0.6 | C19H20NO10 | 246.0759, 231.0525, 213.0425, 185.0471 | P U | Glucuronidation of M5 |
M9 | 8.9 | 422.1076 | −1.3 | C19H20NO10 | 246.0759, 231.0525, 216.0289, 188.0339 | P U | Glucuronidation of M6 |
M10 | 11.3 | 422.1087 | 1.3 | C19H20NO10 | 246.0760, 231.0530, 216.0297 | P U | Glucuronidation of M7 |
M11 | 11.7 | 326.0332 | 0.9 | C13H12NO7S | 246.0759, 231.0525, 216.0289, 188.0339 | P U | Sulfation of M6 |
M12 | 14.4 | 326.0335 | 1.9 | C13H12NO7S | 246.0759, 231.0525, 216.0289, 188.0339 | P U | Sulfation of M7 |
M13 | 12.0 | 356.0438 | 1.0 | C14H14NO8S | 276.0866, 261.0622, 243.0527, 200.0344 | P U | Sulfation of M3 |
M14 | 6.1 | 232.0604 | −0.4 | C12H10NO4 | 217.0370, 189.0420, 161.0473 | P U F | C4, C8-O-Didemethylation of skimmianine |
M15 | 8.5 | 232.0601 | −1.5 | C12H10NO4 | 217.0369, 189.0404, 161.0476 | U | C4, C7-O-Didemethylation of skimmianine |
M16 | 14.9 | 232.0603 | −0.7 | C12H10NO4 | 217.0371, 189.0427, 161.0468 | P U | C7, C8-O-Didemethylation of skimmianine |
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Huang, A.; Xu, H.; Zhan, R.; Chen, W.; Liu, J.; Chi, Y.; Chen, D.; Ji, X.; Luo, C. Metabolic Profile of Skimmianine in Rats Determined by Ultra-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry. Molecules 2017, 22, 489. https://doi.org/10.3390/molecules22040489
Huang A, Xu H, Zhan R, Chen W, Liu J, Chi Y, Chen D, Ji X, Luo C. Metabolic Profile of Skimmianine in Rats Determined by Ultra-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry. Molecules. 2017; 22(4):489. https://doi.org/10.3390/molecules22040489
Chicago/Turabian StyleHuang, Aihua, Hui Xu, Ruoting Zhan, Weiwen Chen, Jiawei Liu, Yuguang Chi, Daidi Chen, Xiaoyu Ji, and Chaoquan Luo. 2017. "Metabolic Profile of Skimmianine in Rats Determined by Ultra-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry" Molecules 22, no. 4: 489. https://doi.org/10.3390/molecules22040489