Constituents of the Roots of Dichapetalum pallidum and Their Anti-Proliferative Activity
Abstract
:1. Introduction
2. Results
2.1. Characterization of Compounds
2.2. Effect of Compounds 1–5 on Cell Proliferation of Leukemia Cancer Cell Lines
3. Conclusions
4. Materials and Methods
4.1. General Experimental Procedures
4.2. Plant Material
4.3. Extraction and Isolation
4.4. Cytotoxicity Assay
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds 2–8 are available from the authors. |
No. | 1 (in CDCl3, 600 MHz) | 2 (in CDCl3, 600 MHz) | 3 (in CDCl3, 500 MHz) | |||
---|---|---|---|---|---|---|
δc, Type | δH (J/Hz) | δc, Type | δH (J/Hz) | δc, Type | δH (J/Hz) | |
1a | 1.68, m | 1.66, m | 1.69, m | |||
1b | 40.1, CH2 | 2.03, dd (7.1, 16.1) | 40.1, CH2 | 2.10, dd (7.0, 16.2) | 40.1, CH2 | 2.11, dd (7.1, 16.2) |
2 | 117.8, CH | 5.42, d (7.1) | 117.7, CH | 5.40, d (6.9) | 117.8, CH | 5.43, ddd (1.8, 1.8, 7.1) |
3 | 140.0, C | 140.1, C | 140.0, C | |||
4 | 38.3, C | 38.3, C | 38.3, C | |||
5 | 43.7, CH | 2.01, m | 43.8, CH | 2.00, m | 43.8, CH | 2.04, m |
6a | 1.62, ddd (2.4, 13.8, 13.8) | 1.62, m | 1.67, m | |||
6b | 24.1, CH2 | 1.85, ddd (3.0, 3.0, 13.8) | 24.2, CH2 | 1.85, dd (10.5, 12.7) | 24.2, CH2 | 1.86, ddd (3.0, 3.0, 13.8) |
7 | 72.3, CH | 3.95, dd (2.4, 3.0) | 72.3, CH | 3.94, m | 72.3, CH | 3.97, dd (2.4, 3.0) |
8 | 36.4, C | 36.4, C | 36.4, C | |||
9 | 45.7, CH | 2.01, m | 45.7, CH | 1.98, m | 45.7, CH | 2.02, m |
10 | 35.7, C | 35.2, C | 35.7, C | |||
11 | 124.1, CH | 5.46, dd (2.5, 10.2) | 124.1, CH | 5.45, dd (2.1, 10.0) | 124.0, CH | 5.48, dd (2.6, 10.2) |
12 | 129.1, CH | 6.20, dd (3.1, 10.2) | 129.0, CH | 6.14, dd (2.9, 10.0) | 129.1, CH | 6.21, dd (3.1, 10.2) |
13 | 29.9, C | 30.1, C | 30.0, C | |||
14 | 36.2, C | 36.2, C | 36.3, C | |||
15a | 1.73, m | |||||
15b | 24.8, CH2 | 2.05, m | 24.9, CH2 | 2.05, d (12.2) | 24.8, CH2 | 2.08, m |
16a | 1.27, m | |||||
16b | 23.2, CH2 | 1.78, ddd (9.6, 15.6, 19.2) | 22.8, CH2 | 1.12, m | 23.2, CH2 | 1.78, ddd (9.6, 15.6, 19.2) |
17 | 40.3, CH | 2.63, m | 41.0, CH | 2.62, m | 40.3, CH | 2.64, m |
18 | 17.5, CH3 | 0.92, s | 17.4, CH3 | 0.90, s | 17.5, CH3 | 0.92, s |
19 | 18.2, CH3 | 1.08, s | 18.1, CH3 | 1.08, s | 18.2, CH3 | 1.11, s |
20 | 47.1, CH | 3.01, dd (5.1, 10.0) | 42.1, CH | 3.08, ddd (4.9, 8.3, 12.9) | 47.0, CH | 3.05, dd (5.1, 10.0) |
21 | 173.9, C | 178.2, C | 173.9, C | |||
22 | 71.9, CH | 4.18, t (9.6) | 31.4, CH2 | 2.37, m | 72.1, CH | 4.19, d (9.5) |
23 | 111.3, C | 75.1, CH | 5.12, m | 111.4, C | ||
24a | 2.51, d (15.1) | 2.53, d (15.2) | ||||
24b | 45.7, CH2 | 2.84, d (15.1) | 122.0, CH | 5.52, dd (8.3, 1.0) | 45.8, CH2 | 2.91, d (15.2) |
25 | 85.0, C | 141.7, C | 86.0, C | |||
26a | 4.09, d (10.2) | 4.09, d (10.2) | ||||
26b | 78.9, CH2 | 4.32, d (10.2) | 67.2, CH2 | 4.05, s | 78.3, CH2 | 4.37, d (10.2) |
27 | 22.0, CH3 | 1.70, s | 14.1, CH3 | 1.74, s | 21.6, CH3 | 1.74, s |
28 | 23.8, CH3 | 1.33, s | 23.8, CH3 | 1.32, s | 23.8, CH3 | 1.36, s |
30a | 0.93, d (5.3) | 0.78, d (5.2) | 0.96, d (5.3) | |||
30b | 15.4, CH2 | 1.29, m | 15.0, CH2 | 1.18, d (4.6) | 15.5, CH2 | |
2′a | 3.62, d (10.5) | 3.59, d (10.6) | 3.63, d (10.5) | |||
2′b | 72.5, CH2 | 3.80, d (10.5) | 72.5, CH2 | 3.76, d (10.6) | 72.5, CH2 | 3.80, d (10.5) |
5′a | 2.20, dd | 2.19, dd (2.4, 13.4) | 2.23, dd (2.7, 13.7) | |||
5′b | 40.7, CH2 | 2.63, m | 40.7, CH2 | 2.62, m | 40.8, CH2 | |
6′ | 81.8, CH | 4.28, dd (2.5, 9.1) | 81.8, CH | 4.26, dd (2.3, 11.6) | 81.8, CH | 4.29, dd (3.6, 11.6) |
1″ | 142.6, C | 142.6, C | 142.6, C | |||
2″,6′′ | 125.8, CH | 7.35, m | 125.8, CH | 7.37, m | 125.8, CH | 7.36, m |
3″,5″ | 128.4, CH | 7.33, m | 128.3, CH | 7.33, m | 128.4, CH | 7.40, m |
4″ | 127.5, CH | 7.24, m | 127.5, CH | 7.25, m | 127.5, CH | 7.28, m |
1′′′ | 170.4, C | 172.1, C | ||||
2′′′a | 21.8, CH3 | 2.03, s | 2.40, dd (9.2, 16.2) | |||
2′′′b | 43.7, CH2 | 2.47, dd (3.6, 16.2) | ||||
3′′′ | 64.6, CH | 4.20, m | ||||
4′′′ | 22.7, CH3 | 1.26, d (6.1) |
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Osei-Safo, D.; Dziwornu, G.A.; Appiah-Opong, R.; Chama, M.A.; Tuffour, I.; Waibel, R.; Amewu, R.; Addae-Mensah, I. Constituents of the Roots of Dichapetalum pallidum and Their Anti-Proliferative Activity. Molecules 2017, 22, 532. https://doi.org/10.3390/molecules22040532
Osei-Safo D, Dziwornu GA, Appiah-Opong R, Chama MA, Tuffour I, Waibel R, Amewu R, Addae-Mensah I. Constituents of the Roots of Dichapetalum pallidum and Their Anti-Proliferative Activity. Molecules. 2017; 22(4):532. https://doi.org/10.3390/molecules22040532
Chicago/Turabian StyleOsei-Safo, Dorcas, Godwin Akpeko Dziwornu, Regina Appiah-Opong, Mary Anti Chama, Isaac Tuffour, Reiner Waibel, Richard Amewu, and Ivan Addae-Mensah. 2017. "Constituents of the Roots of Dichapetalum pallidum and Their Anti-Proliferative Activity" Molecules 22, no. 4: 532. https://doi.org/10.3390/molecules22040532