Pamidronate-Conjugated Biodegradable Branched Copolyester Carriers: Synthesis and Characterization
Abstract
:1. Introduction
2. Results
2.1. Copolymeric Branched Matrices Synthesis and Characterization
2.2. In vitro PAM Release Characteristic and the Branched Copolymer Conjugates Synthesis
3. Materials and Methods
3.1. General Information
3.2. Synthesis of the Branched Copolymeric Carriers
3.3. Spectral Characterization of Copolymeric Biodegradable Matrices (bis-MPA-PLLA90-PKL38 (1H-NMR and FT-IR) and bis-MPA-PLLA38-PCL90 (13C-NMR) as Examples)
3.4. Synthesis of the bis-MPA-PLLA50-PCL50-PAM, bis-MPA-PLLA90-PCL38-PAM and bis-MPA-PLLA38-PCL90-PAM Branched Conjugates
3.5. Toxicity Assays
3.5.1. Microtox Assay
3.5.2. Spirotox Test
3.5.3. The Umu-Test
3.6. Fabrication of the Hydroxyapatite Granules Doped with Selenite Ion
3.7. Fabrication of the Porous Material—bis-MPA-PLLA-PCL-PAM-hydroxyapatite Doped with Selenium Ion for In Vitro and Hydrolytic Biodegradation Studies
3.8. In Vitro PAM Release Studies form the Synthesized Conjugates Coated Hydroxyapatite Porous Granules Doped with Selenite Ions
3.9. Hydrolytic Degradation of the Synthesized Copolymeric Matrices Coated Hydroxyapatite Porous Granules Doped with Selenite Ion
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
Entry | [I]/[LLA]/[CL] | Yield (%) | DP a | DS a | Mn(SEC-MALLS) b | Mw/Mn b |
---|---|---|---|---|---|---|
bis-MPA-PLLA64-PCL64 | 1/64/64 | 82 | 25 | 13 | 21000 | 1.5 |
bis-MPA-PLLA90-PCL38 | 1/90/38 | 85 | 22 | 10 | 18800 | 1.6 |
bis-MPA-PLLA38-PCL90 | 1/38/90 | 97 | 19 | 11 | 19500 | 1.5 |
Entry | Microtox 15 min-PE 1 0.9 mg mL−1 | Microtox 30 min-PE 1 0.9 mg mL−1 | Spirotox 24 h-PE 1 |
---|---|---|---|
bis-MPA-PLLA64-PCL64 | 10 ± 1 | 11 ± 2 | 0 |
bis-MPA-PLLA90-PCL38 | 14 ± 2 | 18 ± 1 | 0 |
bis-MPA-PLLA38-PCL90 | 4 ± 2 | 5 ± 1 | 0 |
Entry | −S9 | +S9 | ||
---|---|---|---|---|
G ± SD | IR ± SD | G ± SD | IR ± SD | |
bis-MPA-PLLA64-PCL64 | 1.09 ± 0.03 | 0.81 ± 0.09 | 1.18 ± 0.04 | 0.89 ± 0.09 |
bis-MPA-PLLA90-PCL38 | 1.06 ± 0.05 | 0.88 ± 0.07 | 1.16 ± 0.02 | 0.95 ± 0.03 |
bis-MPA-PLLA38-PCL90 | 0.98 ± 0.05 | 0.96 ± 0.02 | 1.15 ± 0.03 | 0.96 ± 0.04 |
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Oledzka, E.; Pachowska, D.; Orłowska, K.; Kolmas, J.; Drobniewska, A.; Figat, R.; Sobczak, M. Pamidronate-Conjugated Biodegradable Branched Copolyester Carriers: Synthesis and Characterization. Molecules 2017, 22, 1063. https://doi.org/10.3390/molecules22071063
Oledzka E, Pachowska D, Orłowska K, Kolmas J, Drobniewska A, Figat R, Sobczak M. Pamidronate-Conjugated Biodegradable Branched Copolyester Carriers: Synthesis and Characterization. Molecules. 2017; 22(7):1063. https://doi.org/10.3390/molecules22071063
Chicago/Turabian StyleOledzka, Ewa, Dagmara Pachowska, Katarzyna Orłowska, Joanna Kolmas, Agata Drobniewska, Ramona Figat, and Marcin Sobczak. 2017. "Pamidronate-Conjugated Biodegradable Branched Copolyester Carriers: Synthesis and Characterization" Molecules 22, no. 7: 1063. https://doi.org/10.3390/molecules22071063