Spatial Overlap of Claudin- and Phosphatidylinositol Phosphate-Binding Sites on the First PDZ Domain of Zonula Occludens 1 Studied by NMR
Abstract
:1. Introduction
2. Results
2.1. ZO-1(PDZ1) as a Phosphoinositide-Binding Domain
2.2. Solution Structure of ZO-1(PDZ1)
2.3. Determination of the CLD- and PIP-Binding Sites by NMR Titration Experiments
2.4. Spatial Overlap of the PIP-Binding Site and CLD C-Terminal Peptide-Binding Site on ZO-1(PDZ1) Causes Competitive Binding of Phytic Acid to the ZO-1(PDZ1)-CLD Interaction
2.5. Identification of Key Residues of ZO-1(PDZ1) That Recognize CLD
3. Discussion
4. Materials and Methods
4.1. Protein Expression and Purification
4.2. PIP Strip Assays
4.3. NMR Spectroscopy
4.4. NMR Titration Experiments
4.5. Surface Plasmon Resonance (SPR) Analysis
4.6. Mutation Studies and CLD-3 Binding Assays
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Expression vectors of ZO-1(PDZ) and its mutants are available from the authors. |
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Hiroaki, H.; Satomura, K.; Goda, N.; Nakakura, Y.; Hiranuma, M.; Tenno, T.; Hamada, D.; Ikegami, T. Spatial Overlap of Claudin- and Phosphatidylinositol Phosphate-Binding Sites on the First PDZ Domain of Zonula Occludens 1 Studied by NMR. Molecules 2018, 23, 2465. https://doi.org/10.3390/molecules23102465
Hiroaki H, Satomura K, Goda N, Nakakura Y, Hiranuma M, Tenno T, Hamada D, Ikegami T. Spatial Overlap of Claudin- and Phosphatidylinositol Phosphate-Binding Sites on the First PDZ Domain of Zonula Occludens 1 Studied by NMR. Molecules. 2018; 23(10):2465. https://doi.org/10.3390/molecules23102465
Chicago/Turabian StyleHiroaki, Hidekazu, Kaori Satomura, Natsuko Goda, Yukako Nakakura, Minami Hiranuma, Takeshi Tenno, Daizo Hamada, and Takahisa Ikegami. 2018. "Spatial Overlap of Claudin- and Phosphatidylinositol Phosphate-Binding Sites on the First PDZ Domain of Zonula Occludens 1 Studied by NMR" Molecules 23, no. 10: 2465. https://doi.org/10.3390/molecules23102465