A Near-Infrared Fluorescent Probe Based on a FRET Rhodamine Donor Linked to a Cyanine Acceptor for Sensitive Detection of Intracellular pH Alternations
Abstract
:1. Introduction
2. Results
2.1. Synthesis of Fluorescent Probes
2.2. Optical Responeses of Fluorescent Probes to pH Changes
2.3. Selectivity the Probes to pH over Cations, Anions, and Amino Acids
2.4. Theoretical Results
2.5. Cellular Fluorescence Imaging
3. Discussion
4. Materials and Methods
4.1. Synthesis of Probe A
4.2. Synthesis of Probe B+
4.3. Theoretical Calculations
4.4. Cell Culture and Cell Imaging Procedures
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are all available from the authors. |
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Zhang, Y.; Bi, J.; Xia, S.; Mazi, W.; Wan, S.; Mikesell, L.; Luck, R.L.; Liu, H. A Near-Infrared Fluorescent Probe Based on a FRET Rhodamine Donor Linked to a Cyanine Acceptor for Sensitive Detection of Intracellular pH Alternations. Molecules 2018, 23, 2679. https://doi.org/10.3390/molecules23102679
Zhang Y, Bi J, Xia S, Mazi W, Wan S, Mikesell L, Luck RL, Liu H. A Near-Infrared Fluorescent Probe Based on a FRET Rhodamine Donor Linked to a Cyanine Acceptor for Sensitive Detection of Intracellular pH Alternations. Molecules. 2018; 23(10):2679. https://doi.org/10.3390/molecules23102679
Chicago/Turabian StyleZhang, Yibin, Jianheng Bi, Shuai Xia, Wafa Mazi, Shulin Wan, Logan Mikesell, Rudy L. Luck, and Haiying Liu. 2018. "A Near-Infrared Fluorescent Probe Based on a FRET Rhodamine Donor Linked to a Cyanine Acceptor for Sensitive Detection of Intracellular pH Alternations" Molecules 23, no. 10: 2679. https://doi.org/10.3390/molecules23102679