Unfractionated and Low Molecular Weight Heparin Reduce Platelet Induced Epithelial-Mesenchymal Transition in Pancreatic and Prostate Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. Interaction between Different Tumor Cells and Platelets and the Impact of UFH and LMWH
2.2. Impact of AsPC-1 and PC-3 Cell Induced Platelet Activation on Hepatocyte Growth Factor (HGF) and Platelet-Derived Growth Factor (PDGF) Granule Secretion
2.3. Impact of AsPC-1 and PC-3 Cell Induced Platelet Activation on Epidermal Growth Factor and Transforming Growth Factor Beta 1 Granule Release
2.4. Interaction of Platelets with Tumor Cells Induces a Mesenchymal Phenotype
2.5. Platelet Treatement Induces an Invasive and Promigratory Phenotype in Tumor Cells
3. Discussion
4. Materials and Methods
4.1. Cell Lines
4.2. Platelet Isolation and Activation
4.3. EMT Induction
4.4. Adhesion Assay
4.5. ELISA
4.6. qRT-PCR
4.7. Western Blot
4.8. Wound Healing/Scratch Assay
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds UFH and Enoxaparin are available from the authors. |
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Ponert, J.M.; Gockel, L.M.; Henze, S.; Schlesinger, M. Unfractionated and Low Molecular Weight Heparin Reduce Platelet Induced Epithelial-Mesenchymal Transition in Pancreatic and Prostate Cancer Cells. Molecules 2018, 23, 2690. https://doi.org/10.3390/molecules23102690
Ponert JM, Gockel LM, Henze S, Schlesinger M. Unfractionated and Low Molecular Weight Heparin Reduce Platelet Induced Epithelial-Mesenchymal Transition in Pancreatic and Prostate Cancer Cells. Molecules. 2018; 23(10):2690. https://doi.org/10.3390/molecules23102690
Chicago/Turabian StylePonert, Jan Moritz, Lukas Maria Gockel, Svenja Henze, and Martin Schlesinger. 2018. "Unfractionated and Low Molecular Weight Heparin Reduce Platelet Induced Epithelial-Mesenchymal Transition in Pancreatic and Prostate Cancer Cells" Molecules 23, no. 10: 2690. https://doi.org/10.3390/molecules23102690