Selenate Prevents Adipogenesis through Induction of Selenoprotein S and Attenuation of Endoplasmic Reticulum Stress
Abstract
:1. Introduction
2. Results
2.1. Selenate Pretreatment Suppresses Adipogenesis in 3T3-L1 Preadipocytes
2.2. Selenate Pretreatment Inhibits PPARγ and C/EBPα Gene Expression in the Early Phase of Adipogenesis
2.3. Regulation of SEPS1 Protein by Selenium and Dexamethasone Treatment
2.4. Selenate Pretreatment Inhibits ER Stress Marker Gene Expression
3. Discussion
4. Materials and Methods
4.1. Materials and Reagents
4.2. Cell Culture and Differentiations of 3T3-L1 Preadipocytes
4.3. Isolation of Total RNA and Quantitative RT-PCR
4.4. Immunoblot Analysis
4.5. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Kim, C.Y.; Kim, K.-H. Selenate Prevents Adipogenesis through Induction of Selenoprotein S and Attenuation of Endoplasmic Reticulum Stress. Molecules 2018, 23, 2882. https://doi.org/10.3390/molecules23112882
Kim CY, Kim K-H. Selenate Prevents Adipogenesis through Induction of Selenoprotein S and Attenuation of Endoplasmic Reticulum Stress. Molecules. 2018; 23(11):2882. https://doi.org/10.3390/molecules23112882
Chicago/Turabian StyleKim, Choon Young, and Kee-Hong Kim. 2018. "Selenate Prevents Adipogenesis through Induction of Selenoprotein S and Attenuation of Endoplasmic Reticulum Stress" Molecules 23, no. 11: 2882. https://doi.org/10.3390/molecules23112882