Modulation of ERK1/2 and Akt Pathways Involved in the Neurotrophic Action of Caffeic Acid Alkyl Esters
Abstract
:1. Introduction
2. Results
2.1. Chemistry
2.2. Effect of K252a and Caffeic Acid Ester Derivatives on the Viability of Serum-Deprived PC12 Cells
2.3. Assessment of TrkA Phosphorylation in PC12 Cells
2.4. Establishment of NIH/3T3-TrkA Cells
2.5. Assessment of TrkA Phosphorylation in NIH/3T3-TrkA Cells
2.6. Assessment of ERK1/2 and Akt Phosphorylation in PC12 Cells
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Chemistry
4.3. Cell Culture
4.4. Cell Viability Assay Using K252a
4.5. Immunoblotting
4.6. Establishment of Stable TrkA Overexpressing NIH/3T3 Cells
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
Compound | Structure |
---|---|
CAF1: C1 | |
CAF2: C2 | |
CAF3: C3 | |
CAF4: C4 | |
CAF5: C6 | |
CAF6: C8 | |
CAF7: C10 | |
CAF8: C12 |
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Hosseini, R.; Moosavi, F.; Silva, T.; Rajaian, H.; Hosseini, S.Y.; Bina, S.; Saso, L.; Miri, R.; Borges, F.; Firuzi, O. Modulation of ERK1/2 and Akt Pathways Involved in the Neurotrophic Action of Caffeic Acid Alkyl Esters. Molecules 2018, 23, 3340. https://doi.org/10.3390/molecules23123340
Hosseini R, Moosavi F, Silva T, Rajaian H, Hosseini SY, Bina S, Saso L, Miri R, Borges F, Firuzi O. Modulation of ERK1/2 and Akt Pathways Involved in the Neurotrophic Action of Caffeic Acid Alkyl Esters. Molecules. 2018; 23(12):3340. https://doi.org/10.3390/molecules23123340
Chicago/Turabian StyleHosseini, Razieh, Fatemeh Moosavi, Tiago Silva, Hamid Rajaian, Seyed Younes Hosseini, Samaneh Bina, Luciano Saso, Ramin Miri, Fernanda Borges, and Omidreza Firuzi. 2018. "Modulation of ERK1/2 and Akt Pathways Involved in the Neurotrophic Action of Caffeic Acid Alkyl Esters" Molecules 23, no. 12: 3340. https://doi.org/10.3390/molecules23123340