Analysis of Protein-Phenolic Compound Modifications Using Electrochemistry Coupled to Mass Spectrometry
Abstract
:1. Introduction
2. Results
2.1. Electrochemical Oxidation of Phenolic Acids by Means of EC/ESI-MS
2.2. Comparison of the Oxidation of Various Phenolic Acids
2.3. Investigation of Adduct Formation of Chlorogenic Acid and Alpha-Lactalbumin Using EC/LC/ESI-MS
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Electrochemical Oxidation of Phenolic Acids by Means of EC/MS
4.3 Tryptic Digestion of Alpha-Lactalbumin
4.4 Investigation of Adduct Formation of Chlorogenic Acid and Alpha-Lactalbumin Using EC/LC/ESI-MS
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available. |
Fragments [m/z] | Position | Sequence |
---|---|---|
1198.6 | 118-127 | Val-Gly-Ile-Asn-Tyr-Trp-Leu-Ala-His-Lys |
749.4 | 113-118 | Glu-Leu-Lys-Asp-Leu-Lys |
652.3 | 25-29 | Cys-Glu-Val-Phe-Arg |
616.3 | 20-24 | Glu-Gln-Leu-Thr-Lys |
544.3 | 78-81 | Ile-Trp-Cys-Lys |
486.3 | 114-117 | Ile-Leu-Asp-Lys |
387.2 | 30-32 | Glu-Leu-Lys |
373.2 | 33-35 | Asp-Leu-Lys |
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Kallinich, C.; Schefer, S.; Rohn, S. Analysis of Protein-Phenolic Compound Modifications Using Electrochemistry Coupled to Mass Spectrometry. Molecules 2018, 23, 264. https://doi.org/10.3390/molecules23020264
Kallinich C, Schefer S, Rohn S. Analysis of Protein-Phenolic Compound Modifications Using Electrochemistry Coupled to Mass Spectrometry. Molecules. 2018; 23(2):264. https://doi.org/10.3390/molecules23020264
Chicago/Turabian StyleKallinich, Constanze, Simone Schefer, and Sascha Rohn. 2018. "Analysis of Protein-Phenolic Compound Modifications Using Electrochemistry Coupled to Mass Spectrometry" Molecules 23, no. 2: 264. https://doi.org/10.3390/molecules23020264