Structure-Antiplatelet Activity Relationships of Novel Ruthenium (II) Complexes: Investigation of Its Molecular Targets
Abstract
:1. Introduction
2. Results
2.1. Molecular Structure of Complexes
2.2. Electronic Spectra
2.3. Influence of TQ-1, 2 and 3 on Collagen and Thrombin-Induced Platelet Aggregation
2.4. Effect of TQ-1, 2 and 3 on Cytotoxicity, [Ca2+]i Mobilization and ATP Release in Human Platelets
2.5. TQ-1, 2 and 3 on Collagen-Induced P-Selectin Expression
2.6. The Kinase Activities of the Src-Family Kinases Fyn and Lyn and the Tyrosine Kinase Syk are Inhibited by TQ-3
2.7. TQ-3 Attenuated Collagen Stimulated Akt, JNK and p38 MAPK Phosphorylation in Platelets
3. Discussion
4. Methods
4.1. Reagents
4.2. Synthesis of Ligands (L1–L3)
4.3. Synthesis of TQ1, TQ2 and TQ3
4.4. Platelet Aggregation and ATP Release
4.5. Detection of Lactate Dehydrogenase
4.6. Measurement of Relative Ca2+ Mobilization by Fura 2-AM Fluorescence
4.7. Immunoblotting
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds TQ-1, TQ-2 and TQ-3 are available from the authors. |
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Hsia, C.-H.; Jayakumar, T.; Sheu, J.-R.; Tsao, S.-Y.; Velusamy, M.; Hsia, C.-W.; Chou, D.-S.; Chang, C.-C.; Chung, C.-L.; Khamrang, T.; et al. Structure-Antiplatelet Activity Relationships of Novel Ruthenium (II) Complexes: Investigation of Its Molecular Targets. Molecules 2018, 23, 477. https://doi.org/10.3390/molecules23020477
Hsia C-H, Jayakumar T, Sheu J-R, Tsao S-Y, Velusamy M, Hsia C-W, Chou D-S, Chang C-C, Chung C-L, Khamrang T, et al. Structure-Antiplatelet Activity Relationships of Novel Ruthenium (II) Complexes: Investigation of Its Molecular Targets. Molecules. 2018; 23(2):477. https://doi.org/10.3390/molecules23020477
Chicago/Turabian StyleHsia, Chih-Hsuan, Thanasekaran Jayakumar, Joen-Rong Sheu, Shin-Yi Tsao, Marappan Velusamy, Chih-Wei Hsia, Duen-Suey Chou, Chao-Chien Chang, Chi-Li Chung, Themmila Khamrang, and et al. 2018. "Structure-Antiplatelet Activity Relationships of Novel Ruthenium (II) Complexes: Investigation of Its Molecular Targets" Molecules 23, no. 2: 477. https://doi.org/10.3390/molecules23020477