Phycocyanin Protects Against UVB-induced Apoptosis Through the PKC α/βII-Nrf-2/HO-1 Dependent Pathway in Human Primary Skin Cells
Abstract
:1. Introduction
2. Results
2.1. Pc-Induces HO-1 mRNA and Protein Expression without Cytotoxicity in Human Primary Skin Cells
2.2. Pc-Induced HO-1 Expression Is Mediated by Nrf-2
2.3. Pc Protects against UVB-Induced Apoptotic Cell Death in Primary Skin Cells
2.4. Induction of HO-1 and Activation of Nrf-2 by Pc via Phosphorylation of PKC α/β II
3. Discussion
4. Material and Methods
4.1. Reagents
4.2. Primary Cell Culture
4.3. UVB Radiation
4.4. RNA Interference
4.5. DNA Fragmentation Assay
4.6. Quantitative Reverse Transcription PCR (RT-qPCR)
4.7. Preparation of Cytosolic and Nuclear Fractions
4.8. Western Blotting
4.9. ARE-Luciferase Assay
4.10. Evaluation of Apoptotic Cell Death
4.11. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Kim, K.M.; Lee, J.Y.; Im, A.-R.; Chae, S. Phycocyanin Protects Against UVB-induced Apoptosis Through the PKC α/βII-Nrf-2/HO-1 Dependent Pathway in Human Primary Skin Cells. Molecules 2018, 23, 478. https://doi.org/10.3390/molecules23020478
Kim KM, Lee JY, Im A-R, Chae S. Phycocyanin Protects Against UVB-induced Apoptosis Through the PKC α/βII-Nrf-2/HO-1 Dependent Pathway in Human Primary Skin Cells. Molecules. 2018; 23(2):478. https://doi.org/10.3390/molecules23020478
Chicago/Turabian StyleKim, Ki Mo, Joo Young Lee, A-Rang Im, and Sungwook Chae. 2018. "Phycocyanin Protects Against UVB-induced Apoptosis Through the PKC α/βII-Nrf-2/HO-1 Dependent Pathway in Human Primary Skin Cells" Molecules 23, no. 2: 478. https://doi.org/10.3390/molecules23020478