Antioxidant and Neuroprotective Effects of N-((3,4-Dihydro-2H-benzo[h]chromen-2-yl)methyl)-4-methoxyaniline in Primary Cultured Rat Cortical Cells: Involvement of ERK-CREB Signaling
Abstract
:1. Introduction
2. Results
2.1. Effects of BL-M and Memantine on Glu- or NMDA-Induced Excitotoxicity in Primary Cultured Rat Cortical Cells
2.2. Effects of BL-M and Memantine on Glu- or NMDA-Induced ROS Generation in Primary Cultured Rat Cortical Cells
2.3. Effects of BL-M and Memantine on Lipid Peroxidation and DPPH Radicals
2.4. Effects of BL-M and Memantine on NMDA-Induced Nuclear Translocation of NF-κB in Primary Cultured Rat Cortical Cells
2.5. Effect of BL-M on the Phosphorylation of cAMP Response Element-Binding (CREB) Protein in Primary Cultured Rat Cortical Cells
2.6. Effect of BL-M on the ERK1/2-CREB Signaling in Primary Cultured Rat Cortical Cells
2.7. Effects of Kinase Inhibitors on the Neuroprotective Effect of BL-M in Primary Cultured Rat Cortical Cells
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Preparation of N-((3,4-Dihydro-2H-benzo[h]chromene-2-yl)methyl)-4-methoxyaniline (BL-M)
4.3. Animals
4.4. Primary Culture of Rat Cortical Cells
4.5. Treatment of Cultured Cells
4.6. MTT Assay
4.7. Measurement of Intracellular ROS
4.8. Nuclear and Cytoplasmic Fractionation of Cultured Cells
4.9. Western Blotting
4.10. Immunocytochemistry
4.11. Lipid Peroxidation Assay in Rat Brain Homogenates
4.12. DPPH Radical Scavenging Assay
4.13. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
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Lee, K.; Park, C.; Oh, Y.; Lee, H.; Cho, J. Antioxidant and Neuroprotective Effects of N-((3,4-Dihydro-2H-benzo[h]chromen-2-yl)methyl)-4-methoxyaniline in Primary Cultured Rat Cortical Cells: Involvement of ERK-CREB Signaling. Molecules 2018, 23, 669. https://doi.org/10.3390/molecules23030669
Lee K, Park C, Oh Y, Lee H, Cho J. Antioxidant and Neuroprotective Effects of N-((3,4-Dihydro-2H-benzo[h]chromen-2-yl)methyl)-4-methoxyaniline in Primary Cultured Rat Cortical Cells: Involvement of ERK-CREB Signaling. Molecules. 2018; 23(3):669. https://doi.org/10.3390/molecules23030669
Chicago/Turabian StyleLee, Kyeongjun, Chowee Park, Yeonsoo Oh, Heesoon Lee, and Jungsook Cho. 2018. "Antioxidant and Neuroprotective Effects of N-((3,4-Dihydro-2H-benzo[h]chromen-2-yl)methyl)-4-methoxyaniline in Primary Cultured Rat Cortical Cells: Involvement of ERK-CREB Signaling" Molecules 23, no. 3: 669. https://doi.org/10.3390/molecules23030669