Analysis of Metabolites in White Flowers of Magnolia Denudata Desr. and Violet Flowers of Magnolia Liliiflora Desr.
Abstract
:1. Introduction
2. Results and Discussion
2.1. LC-MS and HPLC Analysis
2.2. Total Phenolic and Anthocyanin Contents
2.3. GC-TOFMS Analysis
2.4. In-Vitro Antioxidant Assays
2.4.1. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) Radical Scavenging Activity
2.4.2. Dismutase-Like Activity
2.5. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide (MTT) Assay for Cell Viability
2.6. Nitric Oxide (NO) Assay
3. Materials and Methods
3.1. Plant Materials
3.2. LC-MS Analysis for the Quantification of Phenylpropanoid Contents
3.3. Phenylpropanoid HPLC Analysis
3.4. Total Phenolics
3.5. Total Anthocyanin Content
3.6. GC-TOFMS Analysis
3.7. Superoxide Dismutase (SOD)-Like Activity
3.8. DPPH Assay
3.9. MTT Assay for Cell Viability
3.10. Nitric Oxide (NO) Assay
3.11. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of compounds are not available from the authors. |
No. | Retention Time | Phenolics | [M − H]− (m/z) | White | Violet |
---|---|---|---|---|---|
1 | 20.98 | 4-hydroxybenzoic acid | 137.6 | 0.27 ± 0.02 | 1.20 ± 0.46 * |
2 | 22.85 | Chlorogenic acid | 353.8 | 0.47 ± 0.10 | 1.69 ± 0.42 * |
3 | 27.78 | Caffeic acid | 179.9 | tr 1 | 0.04 ± 0.00 * |
4 | 41.71 | p-coumaric acid | 163.4 | tr | 0.26 ± 0.04 * |
5 | 61.99 | Rutin | 609.0 | 10.47 ± 0.34 | 13.62 ± 1.45 * |
6 | 73.66 | Quercetin | 301.1 | 0.38 ± 0.00 | 0.37 ± 0.00 |
7 | 80.36 | Kaempferol | 285.5 | 0.12 ± 0.00 | 0.13 ± 0.02 |
Total | 11.71 ± 0.46 | 17.31 ± 2.39 * |
Total Phenolics (mg/g) | Total Anthocyanin (mg/g) | |
---|---|---|
Violet Flowers | 16.94 ± 0.71 * | 0.78 ± 0.16 * |
White Flowers | 14.07 ± 0.55 | 0.21 ± 0.08 |
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Park, C.H.; Park, S.-Y.; Lee, S.Y.; Kim, J.K.; Park, S.U. Analysis of Metabolites in White Flowers of Magnolia Denudata Desr. and Violet Flowers of Magnolia Liliiflora Desr. Molecules 2018, 23, 1558. https://doi.org/10.3390/molecules23071558
Park CH, Park S-Y, Lee SY, Kim JK, Park SU. Analysis of Metabolites in White Flowers of Magnolia Denudata Desr. and Violet Flowers of Magnolia Liliiflora Desr. Molecules. 2018; 23(7):1558. https://doi.org/10.3390/molecules23071558
Chicago/Turabian StylePark, Chang Ha, Soo-Yun Park, Sook Young Lee, Jae Kwang Kim, and Sang Un Park. 2018. "Analysis of Metabolites in White Flowers of Magnolia Denudata Desr. and Violet Flowers of Magnolia Liliiflora Desr." Molecules 23, no. 7: 1558. https://doi.org/10.3390/molecules23071558