Effects of Melatonin on Antioxidant Capacity in Naked Oat Seedlings under Drought Stress
Abstract
:1. Introduction
2. Results
2.1. Effect of Spraying 100 μM MT on Leaves of Naked Oat Seedlings under Drought Stress
2.2. Effect of MT Pretreatment on Antioxidant Enzyme Activities of Naked Oat Seedlings under Drought Stress
2.3. Effect of MT Pretreatment on Changes in ROS in Naked Oat Seedlings under Drought Stress
2.4. Effect of MT Pretreatment on MAPK Activity in Naked Oat Seedlings under Drought Stress
2.5. Effect of MT Pretreatment on the Expression of Antioxidant-Related TF Genes in Naked Oat Seedlings during Drought Stress
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Seed Germination Conditions
4.3. MT Pretreatment
4.4. Drought-Stress Treatment
4.5. Determination of Various Indicators
4.5.1. Calculation of Plant Height, Stem Thickness, Plant Fresh Weight, and Plant Dry Weight
4.5.2. Enzyme Extraction and Assay
4.5.3. Determination of H2O2 and O2−•
4.5.4. Quantitate Real Time-Polymerase Chain Reaction (qRT-PCR) Analysis
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
Abbreviations
MT | Melatonin |
H2O2 | Hydrogen peroxide |
O2−• | Superoxide anion |
SOD | Superoxide dismutase |
POD | Peroxidase |
CAT | Catalase |
APX | Ascorbate peroxidase |
PEG-6000 | Poly-ethylene glycol-6000 |
MAPKs | Mitogen-activated protein kinases |
TF | Transcription factor |
ROS | Reactive oxygen species |
ABA | Abscisic acid |
BRs | Brassinosteroids |
References
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Sample Availability: Samples of the compounds are not available from the authors. |
Gene Name | Sense Primer | Anti-Sense Primer |
---|---|---|
NAC | GGAGTCGGAGATCGTGGACACC | TGGATGTCGTCGTAGCTGAGGTC |
DREB2 | ATACCGTGGTGTGAGGCAG | CGAGATACGAGAAGGAGGA |
WRKY1 | GGCGTCCTCCTTCCTCCAGTC | CCTCGTATGGCGTGCTGAAGC |
MYB | GAACCAGCAGCCGTCTGTGAG | GCAGGAGCGGTGGATTCAGTG |
Asmap1 | CATCCGCTCCAACCAAGAACTCTC | TACTCCGTCATCATGTCGCTCTCC |
Aspk11 | GGTCCATACCCCCACAGA | TAGTCCAACAGCCTCATT |
Actin | ATGTTGCCATCCAGGCTGTG | TAAGTCACGTCCAGCGAGGT |
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Gao, W.; Zhang, Y.; Feng, Z.; Bai, Q.; He, J.; Wang, Y. Effects of Melatonin on Antioxidant Capacity in Naked Oat Seedlings under Drought Stress. Molecules 2018, 23, 1580. https://doi.org/10.3390/molecules23071580
Gao W, Zhang Y, Feng Z, Bai Q, He J, Wang Y. Effects of Melatonin on Antioxidant Capacity in Naked Oat Seedlings under Drought Stress. Molecules. 2018; 23(7):1580. https://doi.org/10.3390/molecules23071580
Chicago/Turabian StyleGao, Wenying, Yujing Zhang, Zheng Feng, Qingqing Bai, Jinjin He, and Yingjuan Wang. 2018. "Effects of Melatonin on Antioxidant Capacity in Naked Oat Seedlings under Drought Stress" Molecules 23, no. 7: 1580. https://doi.org/10.3390/molecules23071580