Transcriptomic Analysis of the Candidate Genes Related to Aroma Formation in Osmanthus fragrans
Abstract
:1. Introduction
2. Results
2.1. Sequencing and De Novo Assembly
2.2. Annotation and Functional Classification
2.3. Analysis of DEGs
2.4. Identification of UnigenesInvolved in Scent Metabolism
2.5. Analysis of TFs during Blooming
2.6. Validation of RNA-Seq Results by qRT-PCR
3. Discussion
4. Materials and Methods
4.1. Plant Materials and RNA Extraction
4.2. Libraries Construction and Illumina Sequencing
4.3. Assembly and Functional Annotation
4.4. Gene Expression Analysis
4.5. qRT-PCR Validation
5. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the petals are available from the authors. |
Transcript | Unigene |
---|---|
Total assembled bases | 108,311,010 |
Total number of genes | 136,611 |
Max length of unigenes (bp) | 16,876 |
Min length of unigenes (bp) | 201 |
Average length ofunigenes (bp) | 792 |
N50 (bp) | 1424 |
GC percentage (%) | 38.9215 |
Database | Number of Annotated Unigenes | Percentage of Annotated Unigenes (%) |
---|---|---|
Nr | 58,556 | 42.86 |
Swiss-prot | 47,294 | 34.62 |
KOG | 39,043 | 28.58 |
KO (KEGG Orthology) | 23,825 | 17.44 |
Total | 64,795 | 47.43 |
KEGG Pathway | All Genes with Pathway Annotation | DGEs Genes with Pathway Annotation | Pathway ID |
---|---|---|---|
Phenylpropanoid | 319 | 178 | Ko00940 |
Monoterpenoid biosynthesis | 17 | 8 | Ko00902 |
Terpenoid backbone biosynthesis | 184 | 78 | Ko00900 |
Sesquiterpenoidand triterpenoid biosynthesis | 69 | 45 | Ko00909 |
Diterpenoid biosynthesis | 45 | 25 | Ko00904 |
Limonene and pinene degradation | 15 | 10 | Ko00903 |
Transcription Factors | Profile0 | Profile1 | Profile4 | Profile6 | Profile7 | All Profiles |
---|---|---|---|---|---|---|
C2H2 | 3 | 2 | 1 | 0 | 2 | 234 |
ERF | 56 | 88 | 50 | 21 | 50 | 193 |
bHLH | 24 | 26 | 13 | 5 | 4 | 168 |
MYB-related | 4 | 6 | 6 | 3 | 5 | 155 |
NAC | 101 | 158 | 110 | 47 | 80 | 134 |
WRKY | 3 | 4 | 41 | 7 | 24 | 112 |
MYB | 18 | 13 | 24 | 9 | 19 | 96 |
bZIP | 4 | 3 | 2 | 0 | 0 | 89 |
GRAS | 2 | 0 | 8 | 11 | 15 | 82 |
C3H | 0 | 1 | 0 | 0 | 2 | 73 |
FAR1 | 3 | 6 | 11 | 6 | 10 | 67 |
Dof | 11 | 3 | 2 | 0 | 1 | 59 |
G2-Like | 3 | 0 | 1 | 1 | 1 | 56 |
HD-ZIP | 0 | 1 | 0 | 0 | 0 | 52 |
Trihelix | 4 | 2 | 2 | 0 | 3 | 50 |
HSF | 2 | 1 | 0 | 1 | 0 | 43 |
TCP | 6 | 11 | 2 | 0 | 7 | 41 |
B3 | 6 | 11 | 10 | 8 | 8 | 40 |
GATA | 14 | 10 | 0 | 1 | 3 | 34 |
SBP | 4 | 5 | 0 | 0 | 0 | 32 |
ARF | 0 | 2 | 2 | 1 | 1 | 29 |
M-type | 4 | 3 | 11 | 5 | 7 | 22 |
NF-YC | 0 | 1 | 0 | 0 | 0 | 16 |
AP2 | 4 | 7 | 5 | 2 | 1 | 14 |
CPP | 0 | 1 | 0 | 0 | 0 | 12 |
WOX | 1 | 1 | 0 | 0 | 0 | 11 |
LSD | 0 | 0 | 1 | 0 | 0 | 9 |
YABBY | 1 | 1 | 0 | 0 | 0 | 8 |
BES1 | 2 | 2 | 0 | 0 | 0 | 8 |
SRS | 1 | 0 | 1 | 2 | 1 | 6 |
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Yang, X.-L.; Li, H.-Y.; Yue, Y.-Z.; Ding, W.-J.; Xu, C.; Shi, T.-T.; Chen, G.-W.; Wang, L.-G. Transcriptomic Analysis of the Candidate Genes Related to Aroma Formation in Osmanthus fragrans. Molecules 2018, 23, 1604. https://doi.org/10.3390/molecules23071604
Yang X-L, Li H-Y, Yue Y-Z, Ding W-J, Xu C, Shi T-T, Chen G-W, Wang L-G. Transcriptomic Analysis of the Candidate Genes Related to Aroma Formation in Osmanthus fragrans. Molecules. 2018; 23(7):1604. https://doi.org/10.3390/molecules23071604
Chicago/Turabian StyleYang, Xiu-Lian, Hai-Yan Li, Yuan-Zheng Yue, Wen-Jie Ding, Chen Xu, Ting-Ting Shi, Gong-Wei Chen, and Liang-Gui Wang. 2018. "Transcriptomic Analysis of the Candidate Genes Related to Aroma Formation in Osmanthus fragrans" Molecules 23, no. 7: 1604. https://doi.org/10.3390/molecules23071604