Potential Mechanism of Action of Cyclosporin A in Human Dermal Fibroblasts—Transcriptomic Analysis of CYPs
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Cell Viability
4.3. Cell Culture with CsA
4.4. RNA Isolation
4.5. Microarray HGU-133A_2.0 Analysis
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Not available. |
Gene Symbol | Fold Change | p Value | SOFM Neuron | Biological Process | ||
---|---|---|---|---|---|---|
8 h vs. 0 | 24 h vs. 0 | 48 h vs. 0 | ||||
CYP19A1 | 1.72 | 1.07 | 1.24 | 0.0006 | 2 | steroids biosynthesis and metabolism |
CYP1B1 | 1.52 | 1.39 | 1.05 | 0.0030 | 2, 3 | steroids metabolism |
CYP2F1 | 1.26 | 1.18 | 1.09 | 0.0050 | 2 | metabolism and synthesis of lipids, cholesterol, steroids |
CYP7A1 | 1.22 | 1.16 | 1.16 | 0.0330 | 2 | steroids biosynthesis |
CYP17A1 | 1.20 | 1.11 | −1.04 | 0.0500 | 2 | biosynthesis of cholesterol, lipids, steroids |
No. | Probe ID | Transcript | NHDFs Control (0) Mean [log2FS] | NHDFs with CsA (8 h) Mean [log2FS] | t Test | SOFM Neuron |
---|---|---|---|---|---|---|
1 | 202436_s_at | 1B1 | 12.24 | 12.64 | ↑ | 2 |
2 | 208131_s_at | 8A1 | 12.15 | 12.27 | NS | |
3 | 202435_s_at | 1B1 | 11.32 | 11.77 | ↑ | 2 |
4 | 202437_s_at | 1B1 | 10.78 | 11.38 | ↑ | 3 |
5 | 216661_x_at | 2C19/9 | 8.62 | 8.62 | NS | |
6 | 209975_at | 2.E1 | 8.46 | 8.46 | NS | |
7 | 202314_at | 51A1 | 8.39 | 8.85 | ↑ | 3 |
8 | 210726_at | 3A4 | 7.96 | 8.03 | NS | |
9 | 219825_at | 26B1 | 7.83 | 7.71 | NS | |
10 | 214234_s_at | 3A5 | 7.81 | 7.90 | NS | |
11 | 216025_x_at | 2C19/9 | 7.75 | 7.60 | NS | |
12 | 209148_at | 2C8 | 7.58 | 7.71 | NS | |
13 | 203979_at | 27A1 | 7.49 | 7.62 | NS | |
14 | 219565_at | 20A1 | 7.47 | 7.49 | NS | |
15 | 205998_x_at | 3A4 | 6.42 | 6.59 | NS | |
16 | 207244_x_at | 2A6 | 6.32 | 6.32 | NS | |
17 | 203475_at | 19A1 | 6.25 | 7.04 | ↑ | 2 |
18 | 220432_s_at | 39A1 | 5.47 | 5.52 | NS | |
19 | 215103_at | 2C18 | 5.47 | 5.34 | NS | |
20 | 214630_at | 11B2 | 5.25 | 5.34 | NS | |
21 | 215982_s_at | 21A2 | 5.23 | 5.33 | NS | |
22 | 214419_s_at | 2C9 | 5.16 | 5.10 | NS | |
23 | 202434_s_at | 1B1 | 5.16 | 5.50 | ↑ | 2 |
24 | 209976_s_at | 2.E1 | 4.94 | 4.75 | NS | |
25 | 207498_s_at | 2D6 | 4.69 | 4.45 | ↓ | 1 |
26 | 216607_s_at | 51A1 | 4.63 | 4.56 | NS | |
27 | 1494_f_at | 2A6 | 4.63 | 4.69 | NS | |
28 | 220562_at | 2W1 | 4.62 | 4.54 | NS | |
29 | 211231_x_at | 4A11 | 4.55 | 4.74 | NS | |
30 | 207386_at | 7B1 | 4.51 | 4.69 | NS | |
31 | 205765_at | 3A5 | 4.49 | 4.34 | NS | |
32 | 208327_at | 2A13 | 4.49 | 4.61 | NS | |
33 | 211440_x_at | 3A43 | 4.46 | 4.65 | NS | |
34 | 210576_at | 4F8 | 4.35 | 4.29 | NS | |
35 | 211295_x_at | 2A6 | 4.33 | 4.49 | NS | |
36 | 220331_at | 46A1 | 4.32 | 4.44 | NS | |
37 | 210452_x_at | 4F2 | 4.24 | 4.17 | NS | |
38 | 207609_s_at | 1A2 | 4.20 | 4.10 | NS | |
39 | 206539_s_at | 4F12 | 4.18 | 4.21 | NS | |
40 | 214320_x_at | 2A6 | 4.17 | 3.88 | NS | |
41 | 204309_at | 11A1 | 4.16 | 4.39 | NS | |
42 | 207608_x_at | 1A2 | 4.14 | 4.44 | ↑ | 3 |
43 | 205502_at | 17A1 | 4.06 | 4.33 | ↑ | 2 |
44 | 1431_at | 2.E1 | 4.01 | 3.67 | NS | |
45 | 216058_s_at | 2C19 | 3.17 | 3.40 | ↑ | 3 |
46 | 207406_at | 7A1 | 2.49 | 2.79 | ↑ | 2 |
47 | 207913_at | 2F1 | 2.51 | 2.86 | ↑ | 2 |
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Janikowska, G.; Pyka-Pająk, A.; Janikowski, T.; Adamska, J.; Mazurek, U.; Jędrusik, P. Potential Mechanism of Action of Cyclosporin A in Human Dermal Fibroblasts—Transcriptomic Analysis of CYPs. Molecules 2018, 23, 1642. https://doi.org/10.3390/molecules23071642
Janikowska G, Pyka-Pająk A, Janikowski T, Adamska J, Mazurek U, Jędrusik P. Potential Mechanism of Action of Cyclosporin A in Human Dermal Fibroblasts—Transcriptomic Analysis of CYPs. Molecules. 2018; 23(7):1642. https://doi.org/10.3390/molecules23071642
Chicago/Turabian StyleJanikowska, Grażyna, Alina Pyka-Pająk, Tomasz Janikowski, Jolanta Adamska, Urszula Mazurek, and Przemysław Jędrusik. 2018. "Potential Mechanism of Action of Cyclosporin A in Human Dermal Fibroblasts—Transcriptomic Analysis of CYPs" Molecules 23, no. 7: 1642. https://doi.org/10.3390/molecules23071642