Selective Extraction and Antioxidant Properties of Thiol-Containing Peptides in Soy Glycinine Hydrolysates
Abstract
:1. Introduction
2. Results and Discussion
2.1. Reduction of Disulfide Bonds in SGHs
2.2. Optimization of the Extraction Conditions of TCPs
2.2.1. Optimization of the Capture Conditions of TCPs
2.2.2. Optimization of the Desorption Conditions of TCPs
2.2.3. Purification of TCPs by Reversed Phase Chromatography Column
2.3. Specificity to TCPs
2.4. Antioxidant Activities of TCPs
3. Materials and Methods
3.1. Materials
3.2. Preparation of Soy Glycinin (11 S)
3.3. Enzymatic Hydrolysis of 11 S
3.4. Reduction of Disulfide Bonds in Soy Glycinine Hydrolysates (SGHs)
3.5. TCPs Enrichment from Reduced SGHs
3.6. Reversed Phase Chromatography
3.7. Sulfhydryl Group Content Measurement
3.8. Size-Exclusion High Performance Chromatography (SEC-HPLC)
3.9. Alkylation of Sulfhydryl Groups and Matrix-Assisted Laser Desorption/Ionization—Time-of-Flight Mass Spectrometry (MALDI-TOF-MS)
3.10. Antioxidant Activities of TCPs
3.10.1. Scavenging Activity against DPPH Radical
3.10.2. Scavenging Activity against Hydroxyl Radical
3.10.3. Superoxide Anion Scavenging Activity Assay
3.10.4. Reducing Power Measurement
3.11. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Not available. |
Pick Number | IAM | NEM | Distance in m/z | Content of Cys |
---|---|---|---|---|
1 | 198.774 | 198.769 | −0.005 | 0 |
2 | 274.156 | 274.149 | −0.007 | 0 |
3 | 284.22 | 284.222 | 0.002 | 0 |
4 | 312.92 | 312.911 | −0.009 | 0 |
5 | 318.22 | 318.202 | −0.018 | 0 |
6 | 833.497 | 833.4 | −0.097 | 0 |
7 | 905.439 | 905.408 | −0.032 | 0 |
8 | 992.494 | 992.485 | −0.009 | 0 |
9 | 1169.603 | 1169.569 | −0.033 | 0 |
10 | 249.849 | 318.202 | 68.353 | 1 |
11 | 315.021 | 383.135 | 68.113 | 1 |
12 | 335.026 | 403.133 | 68.106 | 1 |
13 | 482.176 | 550.227 | 68.051 | 1 |
14 | 517.18 | 585.244 | 68.064 | 1 |
15 | 559.205 | 627.266 | 68.061 | 1 |
16 | 713.328 | 781.374 | 68.046 | 1 |
17 | 765.341 | 833.408 | 68.068 | 1 |
18 | 777.363 | 845.422 | 68.059 | 1 |
19 | 799.351 | 867.417 | 68.066 | 1 |
20 | 815.341 | 883.404 | 68.063 | 1 |
21 | 821.341 | 889.402 | 68.061 | 1 |
22 | 864.424 | 932.471 | 68.047 | 1 |
23 | 910.428 | 978.458 | 68.031 | 1 |
24 | 921.453 | 989.523 | 68.069 | 1 |
25 | 928.422 | 996.486 | 68.064 | 1 |
26 | 940.452 | 1008.478 | 68.026 | 1 |
27 | 992.494 | 1060.551 | 68.056 | 1 |
28 | 1041.518 | 1109.589 | 68.071 | 1 |
29 | 1049.587 | 1117.515 | 67.928 | 1 |
30 | 1063.511 | 1131.587 | 68.076 | 1 |
31 | 1079.487 | 1147.567 | 68.081 | 1 |
32 | 1085.519 | 1153.529 | 68.01 | 1 |
33 | 1150.572 | 1218.581 | 68.009 | 1 |
34 | 1174.575 | 1242.592 | 68.017 | 1 |
35 | 1177.58 | 1245.652 | 68.072 | 1 |
36 | 1188.58 | 1256.645 | 68.065 | 1 |
37 | 1197.631 | 1265.685 | 68.055 | 1 |
38 | 1204.609 | 1272.617 | 68.008 | 1 |
39 | 1210.61 | 1278.59 | 67.981 | 1 |
40 | 1226.607 | 1294.613 | 68.006 | 1 |
41 | 1249.618 | 1317.626 | 68.008 | 1 |
42 | 713.328 | 849.39 | 136.063 | 2 |
43 | 921.453 | 1057.523 | 136.07 | 2 |
44 | 1049.587 | 1185.595 | 136.008 | 2 |
45 | 1125.593 | 1261.639 | 136.046 | 2 |
TCPs | Glutathione | |
---|---|---|
DPPH (mg/mL) A | 0.10 ± 0.004 a | 0.03 ± 0.001 b |
Hydroxyl radical (mg/mL) | 1.49 ± 0.07 a | 0.36 ± 0.014 b |
Superoxide anion radical (mg/mL) | 0.084 ± 0.004 a | 0.052 ± 0.003 b |
Reducing power B | 0.38 ± 0.01 a | 0.57 ± 0.031 b |
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Ding, X.; Kong, X.; Chen, Y.; Zhang, C.; Hua, Y.; Li, X. Selective Extraction and Antioxidant Properties of Thiol-Containing Peptides in Soy Glycinine Hydrolysates. Molecules 2018, 23, 1909. https://doi.org/10.3390/molecules23081909
Ding X, Kong X, Chen Y, Zhang C, Hua Y, Li X. Selective Extraction and Antioxidant Properties of Thiol-Containing Peptides in Soy Glycinine Hydrolysates. Molecules. 2018; 23(8):1909. https://doi.org/10.3390/molecules23081909
Chicago/Turabian StyleDing, Xiuzhen, Xiangzhen Kong, Yeming Chen, Caimeng Zhang, Yufei Hua, and Xiangyang Li. 2018. "Selective Extraction and Antioxidant Properties of Thiol-Containing Peptides in Soy Glycinine Hydrolysates" Molecules 23, no. 8: 1909. https://doi.org/10.3390/molecules23081909