Rational Design for Multicolor Flavone-Based Fluorophores with Aggregation-Induced Emission Enhancement Characteristics and Applications in Mitochondria-Imaging
Abstract
:1. Introduction
2. Results and Discussion
2.1. Optical Properties and Aggregation-Induced Emission Enhancement Property
2.2. Electronic Structure
2.3. Fluorescence Imaging in Living Cells and Cell Viability Assay
2.4. Mitochondrial Imaging
2.5. Mitochondrial Morphological Change
3. Materials and Methods
3.1. Materials and Instruments
3.2. Synthesis of Flavone Derivatives
3.2.1. Synthesis of Compounds 3a–j
3.2.2. Synthesis of Compounds 4a–j
3.2.3. Synthesis of Compounds 5a–j
3.3. Preparation for UV–Vis Spectra, Pl Spectra and SEM Measurements
3.4. Preparation for Ethylene Glycol (EG) Measurement
3.5. Theoretical Calculations
3.6. Cell Viability Evaluation
3.7. Cell Imaging
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds 5a–5j are available from the authors. |
Compound | Solvents | Quantum Yields (ФF) | Time/ns |
---|---|---|---|
CH3OH | 0.16 | 3.15 | |
5a | CH3OH/H2O (3:7) | 0.23 | 3.22 |
CH3OH/H2O (1:9) | 0.19 | 3.19 | |
CH3OH | 0.03 | 2.15 | |
5b | CH3OH/H2O (5:5) | 0.09 | 2.32 |
CH3OH/H2O (1:9) | 0.15 | 3.10 | |
CH3OH | 0.14 | 2.97 | |
5c | CH3OH/H2O (5:5) | 0.22 | 3.29 |
CH3OH/H2O (1:9) | 0.28 | 3.34 | |
CH3OH | 0.05 | 2.18 | |
5d | CH3OH/H2O (5:5) | 0.10 | 2.68 |
CH3OH/H2O (1:9) | 0.02 | 1.67 | |
CH3OH | 0.20 | 3.26 | |
5e | CH3OH/H2O (5:5) | 0.27 | 3.31 |
CH3OH/H2O (1:9) | 0.32 | 3.40 | |
CH3OH | 0.13 | 2.83 | |
5f | CH3OH/H2O (4:6) | 0.18 | 3.16 |
CH3OH/H2O (1:9) | 0.12 | 2.79 |
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Liu, L.; Lei, Y.; Zhang, J.; Li, N.; Zhang, F.; Wang, H.; He, F. Rational Design for Multicolor Flavone-Based Fluorophores with Aggregation-Induced Emission Enhancement Characteristics and Applications in Mitochondria-Imaging. Molecules 2018, 23, 2290. https://doi.org/10.3390/molecules23092290
Liu L, Lei Y, Zhang J, Li N, Zhang F, Wang H, He F. Rational Design for Multicolor Flavone-Based Fluorophores with Aggregation-Induced Emission Enhancement Characteristics and Applications in Mitochondria-Imaging. Molecules. 2018; 23(9):2290. https://doi.org/10.3390/molecules23092290
Chicago/Turabian StyleLiu, Liyan, Yaohui Lei, Jianhui Zhang, Na Li, Fan Zhang, Huaqiao Wang, and Feng He. 2018. "Rational Design for Multicolor Flavone-Based Fluorophores with Aggregation-Induced Emission Enhancement Characteristics and Applications in Mitochondria-Imaging" Molecules 23, no. 9: 2290. https://doi.org/10.3390/molecules23092290