Identification of Pyrvinium, an Anthelmintic Drug, as a Novel Anti-Adipogenic Compound Based on the Gene Expression Microarray and Connectivity Map
Abstract
:1. Introduction
2. Results
2.1. Microarray Analysis Identifies Differential Expression of mRNAs during Adipogenesis in C3H10T1/2 and 3T3-L1 Cells
2.2. Connectivity Map (C-Map) Analysis Identifies Pyrvinium as a Novel Anti-Adipogenic Compound
2.3. Pyrvinium Inhibits C3H10T1/2 and 3T3-L1 Adipogenesis in a Dose-Dependent Manner
2.4. Early Stage Adipogenic Differentiation is Critical for the Inhibitory Action of Pyrvinium
2.5. Pyrvinium Suppresses the Expression of Master Adipogenic Regulators
2.6. Effect of Pyrvinium on the Notch Signaling Pathway during Adipogenic Differentiation
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Adipogenic Differentiation
4.2. Oil Red O Staining
4.3. Microarray Gene Expression Studies and Connectivity Map (C-Map) Analysis
4.4. Cell Viability Assay
4.5. RNA Preparation and Real-Time PCR Analysis
4.6. Western Blot Analysis
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
Rank | C-Map Name | Mean C-Map Score 1 | N 2 | p Value |
---|---|---|---|---|
1 | tanespimycin | −0.392 | 62 | <0.00001 |
2 | LY-294002 | −0.241 | 61 | 0.00002 |
3 | pyrvinium | −0.743 | 6 | 0.00048 |
4 | prochlorperazine | −0.424 | 16 | 0.00170 |
5 | terfenadine | −0.744 | 3 | 0.00172 |
6 | morantel | −0.587 | 5 | 0.00256 |
7 | triflusal | −0.806 | 3 | 0.00272 |
8 | thioridazine | −0.441 | 20 | 0.00552 |
9 | apigenin | −0.684 | 4 | 0.00577 |
10 | Methylbenzethonium chloride | −0.515 | 6 | 0.00683 |
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Wang, Z.; Dai, Z.; Luo, Z.; Zuo, C. Identification of Pyrvinium, an Anthelmintic Drug, as a Novel Anti-Adipogenic Compound Based on the Gene Expression Microarray and Connectivity Map. Molecules 2019, 24, 2391. https://doi.org/10.3390/molecules24132391
Wang Z, Dai Z, Luo Z, Zuo C. Identification of Pyrvinium, an Anthelmintic Drug, as a Novel Anti-Adipogenic Compound Based on the Gene Expression Microarray and Connectivity Map. Molecules. 2019; 24(13):2391. https://doi.org/10.3390/molecules24132391
Chicago/Turabian StyleWang, Zonggui, Zhong Dai, Zhicong Luo, and Changqing Zuo. 2019. "Identification of Pyrvinium, an Anthelmintic Drug, as a Novel Anti-Adipogenic Compound Based on the Gene Expression Microarray and Connectivity Map" Molecules 24, no. 13: 2391. https://doi.org/10.3390/molecules24132391