Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells
Abstract
:1. Introduction
2. Active Feedback 3D Tracking Methods
2.1. Active Feedback 3D Single Particle Tracking with Modified Detection
2.1.1. Tetrahedral Detection Active Feedback Tracking
2.1.2. Multi-Detector Active Feedback Tracking
2.2. Active Feedback 3D Single Particle Tracking with Patterned Excitation
2.2.1. Orbital 3D Tracking Methods
2.2.2. Tetrahedral Excitation 3D Tracking
2.2.3. Dynamic Photon Localization Tracking (3D-DyPLoT)
2.3. Image-Based Active Feedback Tracking
3. Method Comparison
4. Environmental Contextualization in Active Feedback-Based 3D-SPT Methods
5. Active Feedback 3D Single Molecule Tracking: The Final Frontier
6. Outlook
Author Contributions
Funding
Conflicts of Interest
References
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Name | (a) Localization Precision X/Y/Z (nm) | (b) Precision Measurement Sample | (c) Temporal Resolution | (d) Fastest Tracked D (μm2/s) | (e) Count Rate (Fastest D, kHz) | (f) Tracking Duration (Fastest D) | (g) References |
---|---|---|---|---|---|---|---|
Tetrahedral detection | 70/70/110 | Sample: QD | Int.: 5 ms Piezo: ~1 ms | 3 | ~16 | 0.3 s | [69,70] |
Rate: 40 kHz | |||||||
D: 0.37 µm2/s | |||||||
Multi-detector | 9.8/12.8/13.1 | Sample: FNP | Int.: 10 μs Piezo: ~1 ms | 4.81 | - | >8 s | [76] |
Rate: - | |||||||
D: Stationary | |||||||
Orbital (piezo stage) | 2.4/2.8/2.4 | Sample: FNP | Piezo: 32 ms ETL: 8 ms | 0.04 | - | - | [81,82,83] |
Rate: 28 kHz | |||||||
D: Stationary | |||||||
Orbital (laser modulation) | 352/352/272 | Sample: QD | Piezo: 1 ms | 20 | ~120 | 20 s | [88] |
Rate: ~120 kHz | |||||||
D: 20 µm2/s | |||||||
Orbital (bi-plane detection) | 15/15/20 | Sample: FNP | Galvo: 32 ms | 2 | 50 | ~25 s | [84,90] |
Rate: 10–40 kHz | |||||||
D: Stationary | |||||||
Tetrahedral excitation | 60/60/300 | Sample: FNP | Int. 1.86 ms | 12.2 | 473 | 0.078 s | [91] |
Rate: ~5 kHz | |||||||
D: Stationary | |||||||
TSUNAMI | 16.2/16.7/35.1 | Sample: FNP | Piezo: ~1 ms Int.: 1 ms Offline: 50 μs | 7.5 | - | - | [92] |
Rate: ~100 kHz | |||||||
Sample speed: 6 µm2/s | |||||||
3D-DyPLoT | 6.6/8.7/15.6 | Sample: FNP | Int.: 20 μs Piezo: ~1 ms | 20 | 20 | 30 s for particle with 8.9 μm2/s dynamics | [93] |
Rate: ~80 kHz | |||||||
D: Stationary | |||||||
Image-based (bi-plane) | 7/8/27 | Sample: FNP | Int.: 0.3 ms Piezo: 2 ms | 2.4 | 3-4 | 3 s | [100] |
Rate: | |||||||
D: Stationary | |||||||
Image-based (light-sheet) | 10/10/40 | Sample: FNP | Int.: 1.1 ms | 9.4 | - | - | [101] |
Rate: 750 photons | |||||||
D: Stationary |
Method | Tracking Temporal Resolution | Imaging Time | Simultaneous Imaging | Optical Sectioning | Reference |
---|---|---|---|---|---|
TSUNAMI | 50 µs | 0.7 s | NO | YES | [92] |
Orbital | 32 ms | ~200 ms | YES | NO | [84] |
Tetrahedral Detection | 5 ms | 40 ms | YES | YES | [102] |
3D-MM | 10 µs | 1.0 s | YES | YES | [76] |
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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Hou, S.; Johnson, C.; Welsher, K. Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells. Molecules 2019, 24, 2826. https://doi.org/10.3390/molecules24152826
Hou S, Johnson C, Welsher K. Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells. Molecules. 2019; 24(15):2826. https://doi.org/10.3390/molecules24152826
Chicago/Turabian StyleHou, Shangguo, Courtney Johnson, and Kevin Welsher. 2019. "Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells" Molecules 24, no. 15: 2826. https://doi.org/10.3390/molecules24152826