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Peer-Review Record

Validation of an LC-MS/MS Method for the Quantification of Caffeine and Theobromine Using Non-Matched Matrix Calibration Curve

Molecules 2019, 24(16), 2863; https://doi.org/10.3390/molecules24162863
by Vera M. Mendes 1, Margarida Coelho 1,2, Angelo R. Tomé 1,3, Rodrigo A. Cunha 1,4 and Bruno Manadas 1,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Molecules 2019, 24(16), 2863; https://doi.org/10.3390/molecules24162863
Submission received: 11 July 2019 / Accepted: 5 August 2019 / Published: 7 August 2019
(This article belongs to the Section Analytical Chemistry)

Round 1

Reviewer 1 Report

In the revised manuscript the authors acknowledged the insufficient separation of paraxanthine and theophylline and revised the manuscript (e.g. title) accordingly. Overall this study provides a novel solution for quantification of metabolites in biological samples without using blank matrix.  

Reviewer 2 Report

the authors have adequately addressed my concerns

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